Transcriptomic phases of periodontitis lesions using the nonhuman primate model

Abstract We used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic after changes demarcating phases of periodontitis lesions (initiation, progression, resolution). A total of 18 adult Macaca mulatta (12–22 years) had ligatures placed (premolar...

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Autores principales:	Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha Kirakodu, Octavio A. Gonzalez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48c76a8884504a86b2dcc36917ce6dcb
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spelling	oai:doaj.org-article:48c76a8884504a86b2dcc36917ce6dcb2021-12-02T13:41:10ZTranscriptomic phases of periodontitis lesions using the nonhuman primate model10.1038/s41598-021-88803-62045-2322https://doaj.org/article/48c76a8884504a86b2dcc36917ce6dcb2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88803-6https://doaj.org/toc/2045-2322Abstract We used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic after changes demarcating phases of periodontitis lesions (initiation, progression, resolution). A total of 18 adult Macaca mulatta (12–22 years) had ligatures placed (premolar, 1st molar teeth) in all 4 quadrants. Gingival tissue samples were obtained (baseline, 2 weeks, 1 and 3 months during periodontitis and at 5 months resolution). Gene expression was analyzed by microarray [Rhesus Gene 1.0 ST Array (Affymetrix)]. Compared to baseline, a large array of genes were significantly altered at initiation (n = 6049), early progression (n = 4893), and late progression (n = 5078) of disease, with the preponderance being up-regulated. Additionally, 1918 genes were altered in expression with disease resolution, skewed towards down-regulation. Assessment of the genes demonstrated specific profiles of epithelial, bone/connective tissue, apoptosis/autophagy, metabolism, regulatory, immune, and inflammatory responses that were related to health, stages of disease, and tissues with resolved lesions. Unique transcriptomic profiles occured during the kinetics of the periodontitis lesion exacerbation and remission. We delineated phase specific gene expression profiles of the disease lesion. Detection of these gene products in gingival crevicular fluid samples from human disease may contribute to a better understanding of the biological dynamics of the disease to improve patient management.Jeffrey L. EbersoleRadhakrishnan NagarajanSreenatha KirakoduOctavio A. GonzalezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeffrey L. Ebersole Radhakrishnan Nagarajan Sreenatha Kirakodu Octavio A. Gonzalez Transcriptomic phases of periodontitis lesions using the nonhuman primate model
description	Abstract We used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic after changes demarcating phases of periodontitis lesions (initiation, progression, resolution). A total of 18 adult Macaca mulatta (12–22 years) had ligatures placed (premolar, 1st molar teeth) in all 4 quadrants. Gingival tissue samples were obtained (baseline, 2 weeks, 1 and 3 months during periodontitis and at 5 months resolution). Gene expression was analyzed by microarray [Rhesus Gene 1.0 ST Array (Affymetrix)]. Compared to baseline, a large array of genes were significantly altered at initiation (n = 6049), early progression (n = 4893), and late progression (n = 5078) of disease, with the preponderance being up-regulated. Additionally, 1918 genes were altered in expression with disease resolution, skewed towards down-regulation. Assessment of the genes demonstrated specific profiles of epithelial, bone/connective tissue, apoptosis/autophagy, metabolism, regulatory, immune, and inflammatory responses that were related to health, stages of disease, and tissues with resolved lesions. Unique transcriptomic profiles occured during the kinetics of the periodontitis lesion exacerbation and remission. We delineated phase specific gene expression profiles of the disease lesion. Detection of these gene products in gingival crevicular fluid samples from human disease may contribute to a better understanding of the biological dynamics of the disease to improve patient management.
format	article
author	Jeffrey L. Ebersole Radhakrishnan Nagarajan Sreenatha Kirakodu Octavio A. Gonzalez
author_facet	Jeffrey L. Ebersole Radhakrishnan Nagarajan Sreenatha Kirakodu Octavio A. Gonzalez
author_sort	Jeffrey L. Ebersole
title	Transcriptomic phases of periodontitis lesions using the nonhuman primate model
title_short	Transcriptomic phases of periodontitis lesions using the nonhuman primate model
title_full	Transcriptomic phases of periodontitis lesions using the nonhuman primate model
title_fullStr	Transcriptomic phases of periodontitis lesions using the nonhuman primate model
title_full_unstemmed	Transcriptomic phases of periodontitis lesions using the nonhuman primate model
title_sort	transcriptomic phases of periodontitis lesions using the nonhuman primate model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/48c76a8884504a86b2dcc36917ce6dcb
work_keys_str_mv	AT jeffreylebersole transcriptomicphasesofperiodontitislesionsusingthenonhumanprimatemodel AT radhakrishnannagarajan transcriptomicphasesofperiodontitislesionsusingthenonhumanprimatemodel AT sreenathakirakodu transcriptomicphasesofperiodontitislesionsusingthenonhumanprimatemodel AT octavioagonzalez transcriptomicphasesofperiodontitislesionsusingthenonhumanprimatemodel
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Transcriptomic phases of periodontitis lesions using the nonhuman primate model

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