Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model

Background: Liver can be damaged by various factors, including the oxidative stress mechanism. This damage can be inhibited by the application of anti-oxidant. The Annona muricata L. leaf is known to have antioxidant properties. This study aimed to explore the effect of Annona muricata L. leaf extra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kuswinarti Kuswinarti, Ilham Asrori Azka, Dwi Prasetyo
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Padjadjaran 2021
Materias:	annona muricata l. leaf ethanol extract ccl4 hepatocyte Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/48d2757320964b67abe95e1e86359f23
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:48d2757320964b67abe95e1e86359f23
record_format	dspace
spelling	oai:doaj.org-article:48d2757320964b67abe95e1e86359f232021-11-17T05:17:31ZEffect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model2337-433010.15850/amj.v8n2.2225https://doaj.org/article/48d2757320964b67abe95e1e86359f232021-06-01T00:00:00Zhttp://journal.fk.unpad.ac.id/index.php/amj/article/view/2225https://doaj.org/toc/2337-4330Background: Liver can be damaged by various factors, including the oxidative stress mechanism. This damage can be inhibited by the application of anti-oxidant. The Annona muricata L. leaf is known to have antioxidant properties. This study aimed to explore the effect of Annona muricata L. leaf extract against hepatitis in rats. Methods: An experimental study was performed on 25 male Wistar rats that were given Annona muricata L. leaf ethanol extract at 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW for eight days. The CCl4 was administrated on the eighth day and the rats were laparotomized on the next day. The average number of normal hepatocytes was counted in one field of view. This study was conducted at the laboratory of Pharmacology and Therapy of the Faculty of Medicine, Universitas Padjadjaran in September–October 2012. Results: The negative control group showed an intact cell membrane, homogenous cytoplasm, and round nucleus, whereas the positive control group showed necrosis marked by a ruptured cell membrane, vacuolated cytoplasm, and degenerated nucleus. The average number of normal hepatocytes counted in the negative control and the positive group was 404.91±34.09 cells and 23.16±7.84 cells, respectively. In the treatment group, the normal hepatocytes were 95.91±20.96 cells (200 mg/kgBW), 74.53±13.59 cells (400 mg/kgBW) and 46.84±8.46 cells (600 mg/kgBW). Statistically significant inhibition was observed (p<0.05). Conclusions: The Annona muricata L. leaf ethanol extract can inhibit liver tissue damages in hepatitis model rats, possibly due to the presence of acetogenins as an anti-oxidant agent.Kuswinarti KuswinartiIlham Asrori AzkaDwi PrasetyoUniversitas Padjadjaranarticleannona muricata l. leaf ethanol extractccl4hepatocyteMedicineRMedicine (General)R5-920ENAlthea Medical Journal, Vol 8, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	annona muricata l. leaf ethanol extract ccl4 hepatocyte Medicine R Medicine (General) R5-920
spellingShingle	annona muricata l. leaf ethanol extract ccl4 hepatocyte Medicine R Medicine (General) R5-920 Kuswinarti Kuswinarti Ilham Asrori Azka Dwi Prasetyo Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model
description	Background: Liver can be damaged by various factors, including the oxidative stress mechanism. This damage can be inhibited by the application of anti-oxidant. The Annona muricata L. leaf is known to have antioxidant properties. This study aimed to explore the effect of Annona muricata L. leaf extract against hepatitis in rats. Methods: An experimental study was performed on 25 male Wistar rats that were given Annona muricata L. leaf ethanol extract at 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW for eight days. The CCl4 was administrated on the eighth day and the rats were laparotomized on the next day. The average number of normal hepatocytes was counted in one field of view. This study was conducted at the laboratory of Pharmacology and Therapy of the Faculty of Medicine, Universitas Padjadjaran in September–October 2012. Results: The negative control group showed an intact cell membrane, homogenous cytoplasm, and round nucleus, whereas the positive control group showed necrosis marked by a ruptured cell membrane, vacuolated cytoplasm, and degenerated nucleus. The average number of normal hepatocytes counted in the negative control and the positive group was 404.91±34.09 cells and 23.16±7.84 cells, respectively. In the treatment group, the normal hepatocytes were 95.91±20.96 cells (200 mg/kgBW), 74.53±13.59 cells (400 mg/kgBW) and 46.84±8.46 cells (600 mg/kgBW). Statistically significant inhibition was observed (p<0.05). Conclusions: The Annona muricata L. leaf ethanol extract can inhibit liver tissue damages in hepatitis model rats, possibly due to the presence of acetogenins as an anti-oxidant agent.
format	article
author	Kuswinarti Kuswinarti Ilham Asrori Azka Dwi Prasetyo
author_facet	Kuswinarti Kuswinarti Ilham Asrori Azka Dwi Prasetyo
author_sort	Kuswinarti Kuswinarti
title	Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model
title_short	Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model
title_full	Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model
title_fullStr	Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model
title_full_unstemmed	Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model
title_sort	effect of annona muricata l. leaf ethanol extract in ccl4 hepatitis rat model
publisher	Universitas Padjadjaran
publishDate	2021
url	https://doaj.org/article/48d2757320964b67abe95e1e86359f23
work_keys_str_mv	AT kuswinartikuswinarti effectofannonamuricatalleafethanolextractinccl4hepatitisratmodel AT ilhamasroriazka effectofannonamuricatalleafethanolextractinccl4hepatitisratmodel AT dwiprasetyo effectofannonamuricatalleafethanolextractinccl4hepatitisratmodel
_version_	1718425921538490368

Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model

Ejemplares similares