Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome

Background: Exercise improves function, reduces disability, maintains independence, and improves quality of life for low-grade glioma (LGG) patients. Exercise can also improve the effectiveness of cancer treatment. The goal of this research was to find potential exercise related genes that may be us...

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Autores principales:	Lichun Lu, Yifang Hu, Chen Wang, Feng Jiang, Chuyan Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	exercise-related genes methylation prognosis immune cell infiltration low grade glioma Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/48dad8a4319644b7a1a0859ae6fc2b8b
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spelling	oai:doaj.org-article:48dad8a4319644b7a1a0859ae6fc2b8b2021-11-16T07:12:25ZMethylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome2296-889X10.3389/fmolb.2021.747933https://doaj.org/article/48dad8a4319644b7a1a0859ae6fc2b8b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.747933/fullhttps://doaj.org/toc/2296-889XBackground: Exercise improves function, reduces disability, maintains independence, and improves quality of life for low-grade glioma (LGG) patients. Exercise can also improve the effectiveness of cancer treatment. The goal of this research was to find potential exercise related genes that may be used to predict exercise levels and may be used as a biomarker for cancer outcomes.Methods: The GSE111551 database was thoroughly examined in this research, and the resulting conclusion of exercise-related genes was reached. The protein interaction network (PPI) was used to examine the differentially expressed genes (DEGs). Then the exercise-related gene TLR1 was chosen. The expression, methylation degree, prognosis, and immune relevance of TLR1 were investigated using bioinformatics. In addition, we verified the role of TLR1 in Glioma cell lines.Results: LGG patients with reduced TLR1 expression and hypermethylation had a better overall survival (OS) and progression free survival (PFS), using the TCGA database. Low TLR1 expression and hypermethylation of TLR1 were found to be independent biomarkers for OS using Cox regression. Furthermore, the CGGA database was used to confirm the prognostic function of TLR1 in this cancer. Finally, most methylation sites of TLR1 were strongly correlated with immune infiltration and immune checkpoint. Then, reducing TLR1 expression substantially slowed the cell cycle and decreased LGG cell proliferation, emigration, and infiltration in vitro.Conclusions: Exercise-related gene TLR1 has the potential to be a useful prognostic biomarker, and it is thought to be involved in immune cell infiltration and immunotherapy in LGG.Lichun LuYifang HuChen WangFeng JiangChuyan WuFrontiers Media S.A.articleexercise-related genesmethylationprognosisimmune cell infiltrationlow grade gliomaBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	exercise-related genes methylation prognosis immune cell infiltration low grade glioma Biology (General) QH301-705.5
spellingShingle	exercise-related genes methylation prognosis immune cell infiltration low grade glioma Biology (General) QH301-705.5 Lichun Lu Yifang Hu Chen Wang Feng Jiang Chuyan Wu Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome
description	Background: Exercise improves function, reduces disability, maintains independence, and improves quality of life for low-grade glioma (LGG) patients. Exercise can also improve the effectiveness of cancer treatment. The goal of this research was to find potential exercise related genes that may be used to predict exercise levels and may be used as a biomarker for cancer outcomes.Methods: The GSE111551 database was thoroughly examined in this research, and the resulting conclusion of exercise-related genes was reached. The protein interaction network (PPI) was used to examine the differentially expressed genes (DEGs). Then the exercise-related gene TLR1 was chosen. The expression, methylation degree, prognosis, and immune relevance of TLR1 were investigated using bioinformatics. In addition, we verified the role of TLR1 in Glioma cell lines.Results: LGG patients with reduced TLR1 expression and hypermethylation had a better overall survival (OS) and progression free survival (PFS), using the TCGA database. Low TLR1 expression and hypermethylation of TLR1 were found to be independent biomarkers for OS using Cox regression. Furthermore, the CGGA database was used to confirm the prognostic function of TLR1 in this cancer. Finally, most methylation sites of TLR1 were strongly correlated with immune infiltration and immune checkpoint. Then, reducing TLR1 expression substantially slowed the cell cycle and decreased LGG cell proliferation, emigration, and infiltration in vitro.Conclusions: Exercise-related gene TLR1 has the potential to be a useful prognostic biomarker, and it is thought to be involved in immune cell infiltration and immunotherapy in LGG.
format	article
author	Lichun Lu Yifang Hu Chen Wang Feng Jiang Chuyan Wu
author_facet	Lichun Lu Yifang Hu Chen Wang Feng Jiang Chuyan Wu
author_sort	Lichun Lu
title	Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome
title_short	Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome
title_full	Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome
title_fullStr	Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome
title_full_unstemmed	Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome
title_sort	methylation and expression of the exercise-related tlr1 gene is associated with low grade glioma prognosis and outcome
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/48dad8a4319644b7a1a0859ae6fc2b8b
work_keys_str_mv	AT lichunlu methylationandexpressionoftheexerciserelatedtlr1geneisassociatedwithlowgradegliomaprognosisandoutcome AT yifanghu methylationandexpressionoftheexerciserelatedtlr1geneisassociatedwithlowgradegliomaprognosisandoutcome AT chenwang methylationandexpressionoftheexerciserelatedtlr1geneisassociatedwithlowgradegliomaprognosisandoutcome AT fengjiang methylationandexpressionoftheexerciserelatedtlr1geneisassociatedwithlowgradegliomaprognosisandoutcome AT chuyanwu methylationandexpressionoftheexerciserelatedtlr1geneisassociatedwithlowgradegliomaprognosisandoutcome
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Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome

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