Plasmonics Enhanced Smartphone Fluorescence Microscopy

Abstract Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to im...

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Autores principales:	Qingshan Wei, Guillermo Acuna, Seungkyeum Kim, Carolin Vietz, Derek Tseng, Jongjae Chae, Daniel Shir, Wei Luo, Philip Tinnefeld, Aydogan Ozcan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48eddf4a88604aae947adb2062ff5485
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spelling	oai:doaj.org-article:48eddf4a88604aae947adb2062ff54852021-12-02T12:32:43ZPlasmonics Enhanced Smartphone Fluorescence Microscopy10.1038/s41598-017-02395-82045-2322https://doaj.org/article/48eddf4a88604aae947adb2062ff54852017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02395-8https://doaj.org/toc/2045-2322Abstract Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.Qingshan WeiGuillermo AcunaSeungkyeum KimCarolin VietzDerek TsengJongjae ChaeDaniel ShirWei LuoPhilip TinnefeldAydogan OzcanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingshan Wei Guillermo Acuna Seungkyeum Kim Carolin Vietz Derek Tseng Jongjae Chae Daniel Shir Wei Luo Philip Tinnefeld Aydogan Ozcan Plasmonics Enhanced Smartphone Fluorescence Microscopy
description	Abstract Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.
format	article
author	Qingshan Wei Guillermo Acuna Seungkyeum Kim Carolin Vietz Derek Tseng Jongjae Chae Daniel Shir Wei Luo Philip Tinnefeld Aydogan Ozcan
author_facet	Qingshan Wei Guillermo Acuna Seungkyeum Kim Carolin Vietz Derek Tseng Jongjae Chae Daniel Shir Wei Luo Philip Tinnefeld Aydogan Ozcan
author_sort	Qingshan Wei
title	Plasmonics Enhanced Smartphone Fluorescence Microscopy
title_short	Plasmonics Enhanced Smartphone Fluorescence Microscopy
title_full	Plasmonics Enhanced Smartphone Fluorescence Microscopy
title_fullStr	Plasmonics Enhanced Smartphone Fluorescence Microscopy
title_full_unstemmed	Plasmonics Enhanced Smartphone Fluorescence Microscopy
title_sort	plasmonics enhanced smartphone fluorescence microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/48eddf4a88604aae947adb2062ff5485
work_keys_str_mv	AT qingshanwei plasmonicsenhancedsmartphonefluorescencemicroscopy AT guillermoacuna plasmonicsenhancedsmartphonefluorescencemicroscopy AT seungkyeumkim plasmonicsenhancedsmartphonefluorescencemicroscopy AT carolinvietz plasmonicsenhancedsmartphonefluorescencemicroscopy AT derektseng plasmonicsenhancedsmartphonefluorescencemicroscopy AT jongjaechae plasmonicsenhancedsmartphonefluorescencemicroscopy AT danielshir plasmonicsenhancedsmartphonefluorescencemicroscopy AT weiluo plasmonicsenhancedsmartphonefluorescencemicroscopy AT philiptinnefeld plasmonicsenhancedsmartphonefluorescencemicroscopy AT aydoganozcan plasmonicsenhancedsmartphonefluorescencemicroscopy
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Plasmonics Enhanced Smartphone Fluorescence Microscopy

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