Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel

Abstract A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in...

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Autores principales:	Amina Farooq, Nauman Z. Butt, Umer Hassan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48f1611ea250465d9ad43982527b3069
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spelling	oai:doaj.org-article:48f1611ea250465d9ad43982527b30692021-12-02T17:51:06ZBiochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel10.1038/s41598-021-91109-22045-2322https://doaj.org/article/48f1611ea250465d9ad43982527b30692021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91109-2https://doaj.org/toc/2045-2322Abstract A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top–bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top–bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow morphological analysis of various bioparticles in a microfluidic channel in the future.Amina FarooqNauman Z. ButtUmer HassanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amina Farooq Nauman Z. Butt Umer Hassan Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
description	Abstract A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top–bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top–bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow morphological analysis of various bioparticles in a microfluidic channel in the future.
format	article
author	Amina Farooq Nauman Z. Butt Umer Hassan
author_facet	Amina Farooq Nauman Z. Butt Umer Hassan
author_sort	Amina Farooq
title	Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
title_short	Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
title_full	Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
title_fullStr	Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
title_full_unstemmed	Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
title_sort	biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/48f1611ea250465d9ad43982527b3069
work_keys_str_mv	AT aminafarooq biochipwithmultiplanarelectrodesgeometryfordifferentiationofnonsphericalbioparticlesinamicrochannel AT naumanzbutt biochipwithmultiplanarelectrodesgeometryfordifferentiationofnonsphericalbioparticlesinamicrochannel AT umerhassan biochipwithmultiplanarelectrodesgeometryfordifferentiationofnonsphericalbioparticlesinamicrochannel
_version_	1718379283111477248

Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel

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