Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells

Xin-Yuan Sun, Jia-Yun Chen, Chen-Ying Rao, Jian-Ming Ouyang Department of Chemistry, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People’s Republic of ChinaCorrespondence: Jian-Ming OuyangDepartment of Chemistry Institute of Biomineralization a...

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Autores principales:	Sun XY, Chen JY, Rao CY, Ouyang JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cytotoxicity nanocrystal hydroxyapatite crystal size cell injury Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/48f19adab3bd497597e021ae90cf2d54
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spelling	oai:doaj.org-article:48f19adab3bd497597e021ae90cf2d542021-12-02T08:10:42ZSize-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells1178-2013https://doaj.org/article/48f19adab3bd497597e021ae90cf2d542020-07-01T00:00:00Zhttps://www.dovepress.com/size-dependent-cytotoxicity-of-hydroxyapatite-crystals-on-renal-epithe-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xin-Yuan Sun, Jia-Yun Chen, Chen-Ying Rao, Jian-Ming Ouyang Department of Chemistry, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People’s Republic of ChinaCorrespondence: Jian-Ming OuyangDepartment of Chemistry Institute of Biomineralization and Lithiasis Research,Jinan University, Guangzhou 510632, People’s Republic of ChinaTel +86 20-85223353Email toyjm@jnu.edu.cnBackground: Hydroxyapatite (HAP) is a common component of most idiopathic calcium oxalate (CaOx) stones and is often used as a nidus to induce the formation of CaOx kidney stones.Methods: This work comparatively studies the cytotoxicity of four kinds of HAP crystals with different sizes (40 nm to 2 μm), namely, HAP-40 nm, HAP-70 nm, HAP-1 μm, and HAP-2 μm, on human renal proximal tubular epithelial cells (HK-2).Results: HAP crystals reduce the viability and membrane integrity of HK-2 cells in a concentration-dependent manner and consequently cause cytoskeleton damage, cell swelling, increased intracellular reactive oxygen species level, decreased mitochondrial membrane potential, increased intracellular calcium concentration, blocked cell cycle and stagnation in G0/G1 phase, and increased cell necrosis rate. HAP toxicity to HK-2 cells increases with a decrease in crystal size.Conclusion: Cell damage caused by HAP crystals increases the risk of kidney stone formation.Keywords: cytotoxicity, nanocrystal, hydroxyapatite, crystal size, cell injurySun XYChen JYRao CYOuyang JMDove Medical Pressarticlecytotoxicitynanocrystalhydroxyapatitecrystal sizecell injuryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5043-5060 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytotoxicity nanocrystal hydroxyapatite crystal size cell injury Medicine (General) R5-920
spellingShingle	cytotoxicity nanocrystal hydroxyapatite crystal size cell injury Medicine (General) R5-920 Sun XY Chen JY Rao CY Ouyang JM Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells
description	Xin-Yuan Sun, Jia-Yun Chen, Chen-Ying Rao, Jian-Ming Ouyang Department of Chemistry, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People’s Republic of ChinaCorrespondence: Jian-Ming OuyangDepartment of Chemistry Institute of Biomineralization and Lithiasis Research,Jinan University, Guangzhou 510632, People’s Republic of ChinaTel +86 20-85223353Email toyjm@jnu.edu.cnBackground: Hydroxyapatite (HAP) is a common component of most idiopathic calcium oxalate (CaOx) stones and is often used as a nidus to induce the formation of CaOx kidney stones.Methods: This work comparatively studies the cytotoxicity of four kinds of HAP crystals with different sizes (40 nm to 2 μm), namely, HAP-40 nm, HAP-70 nm, HAP-1 μm, and HAP-2 μm, on human renal proximal tubular epithelial cells (HK-2).Results: HAP crystals reduce the viability and membrane integrity of HK-2 cells in a concentration-dependent manner and consequently cause cytoskeleton damage, cell swelling, increased intracellular reactive oxygen species level, decreased mitochondrial membrane potential, increased intracellular calcium concentration, blocked cell cycle and stagnation in G0/G1 phase, and increased cell necrosis rate. HAP toxicity to HK-2 cells increases with a decrease in crystal size.Conclusion: Cell damage caused by HAP crystals increases the risk of kidney stone formation.Keywords: cytotoxicity, nanocrystal, hydroxyapatite, crystal size, cell injury
format	article
author	Sun XY Chen JY Rao CY Ouyang JM
author_facet	Sun XY Chen JY Rao CY Ouyang JM
author_sort	Sun XY
title	Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells
title_short	Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells
title_full	Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells
title_fullStr	Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells
title_full_unstemmed	Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells
title_sort	size-dependent cytotoxicity of hydroxyapatite crystals on renal epithelial cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/48f19adab3bd497597e021ae90cf2d54
work_keys_str_mv	AT sunxy sizedependentcytotoxicityofhydroxyapatitecrystalsonrenalepithelialcells AT chenjy sizedependentcytotoxicityofhydroxyapatitecrystalsonrenalepithelialcells AT raocy sizedependentcytotoxicityofhydroxyapatitecrystalsonrenalepithelialcells AT ouyangjm sizedependentcytotoxicityofhydroxyapatitecrystalsonrenalepithelialcells
_version_	1718398598235815936

Size-Dependent Cytotoxicity of Hydroxyapatite Crystals on Renal Epithelial Cells

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