Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo

Lignin is the second most abundant natural polymer on Earth and is a major cell wall component in vascular plants. Lignin biosynthesis has three stages: biosynthesis, transport, and polymerization of its precursors. However, there is limited knowledge on lignin precursor transport, especially in mon...

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Autores principales:	Natsumi Shimada, Noriaki Munekata, Taku Tsuyama, Yasuyuki Matsushita, Kazuhiko Fukushima, Yoshio Kijidani, Keiji Takabe, Kazufumi Yazaki, Ichiro Kamei
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	membrane transport lignification <i>Phyllostachys pubescens</i> coniferin <i>p</i>-glucocoumaryl alcohol secondary active transport Botany QK1-989
Acceso en línea:	https://doaj.org/article/48f9b4e4c5c644cfa76ff4b8d3cfb1dd
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spelling	oai:doaj.org-article:48f9b4e4c5c644cfa76ff4b8d3cfb1dd2021-11-25T18:44:03ZActive Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo10.3390/plants101122372223-7747https://doaj.org/article/48f9b4e4c5c644cfa76ff4b8d3cfb1dd2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2237https://doaj.org/toc/2223-7747Lignin is the second most abundant natural polymer on Earth and is a major cell wall component in vascular plants. Lignin biosynthesis has three stages: biosynthesis, transport, and polymerization of its precursors. However, there is limited knowledge on lignin precursor transport, especially in monocots. In the present study, we aimed to elucidate the transport mode of lignin monomers in the lignifying tissues of bamboo (<i>Phyllostachys pubescens</i>). The growth manners and lignification processes of bamboo shoots were elucidated, which enabled us to obtain the lignifying tissues reproducibly. Microsomal membrane fractions were prepared from tissues undergoing vigorous lignification to analyze the transport activities of lignin precursors in order to show the ATP-dependent transport of coniferin and <i>p</i>-glucocoumaryl alcohol. The transport activities for both precursors depend on vacuolar type H<sup>+</sup>-ATPase and a H<sup>+</sup> gradient across the membrane, suggesting that the electrochemical potential is the driving force of the transport of both substrates. These findings are similar to the transport properties of these lignin precursors in the differentiating xylem of poplar and Japanese cypress. Our findings suggest that transport of coniferin and <i>p</i>-glucocoumaryl alcohol is mediated by secondary active transporters energized partly by the vacuolar type H<sup>+</sup>-ATPase, which is common in lignifying tissues. The loading of these lignin precursors into endomembrane compartments may contribute to lignification in vascular plants.Natsumi ShimadaNoriaki MunekataTaku TsuyamaYasuyuki MatsushitaKazuhiko FukushimaYoshio KijidaniKeiji TakabeKazufumi YazakiIchiro KameiMDPI AGarticlemembrane transportlignification<i>Phyllostachys pubescens</i>coniferin<i>p</i>-glucocoumaryl alcoholsecondary active transportBotanyQK1-989ENPlants, Vol 10, Iss 2237, p 2237 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	membrane transport lignification <i>Phyllostachys pubescens</i> coniferin <i>p</i>-glucocoumaryl alcohol secondary active transport Botany QK1-989
spellingShingle	membrane transport lignification <i>Phyllostachys pubescens</i> coniferin <i>p</i>-glucocoumaryl alcohol secondary active transport Botany QK1-989 Natsumi Shimada Noriaki Munekata Taku Tsuyama Yasuyuki Matsushita Kazuhiko Fukushima Yoshio Kijidani Keiji Takabe Kazufumi Yazaki Ichiro Kamei Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo
description	Lignin is the second most abundant natural polymer on Earth and is a major cell wall component in vascular plants. Lignin biosynthesis has three stages: biosynthesis, transport, and polymerization of its precursors. However, there is limited knowledge on lignin precursor transport, especially in monocots. In the present study, we aimed to elucidate the transport mode of lignin monomers in the lignifying tissues of bamboo (<i>Phyllostachys pubescens</i>). The growth manners and lignification processes of bamboo shoots were elucidated, which enabled us to obtain the lignifying tissues reproducibly. Microsomal membrane fractions were prepared from tissues undergoing vigorous lignification to analyze the transport activities of lignin precursors in order to show the ATP-dependent transport of coniferin and <i>p</i>-glucocoumaryl alcohol. The transport activities for both precursors depend on vacuolar type H<sup>+</sup>-ATPase and a H<sup>+</sup> gradient across the membrane, suggesting that the electrochemical potential is the driving force of the transport of both substrates. These findings are similar to the transport properties of these lignin precursors in the differentiating xylem of poplar and Japanese cypress. Our findings suggest that transport of coniferin and <i>p</i>-glucocoumaryl alcohol is mediated by secondary active transporters energized partly by the vacuolar type H<sup>+</sup>-ATPase, which is common in lignifying tissues. The loading of these lignin precursors into endomembrane compartments may contribute to lignification in vascular plants.
format	article
author	Natsumi Shimada Noriaki Munekata Taku Tsuyama Yasuyuki Matsushita Kazuhiko Fukushima Yoshio Kijidani Keiji Takabe Kazufumi Yazaki Ichiro Kamei
author_facet	Natsumi Shimada Noriaki Munekata Taku Tsuyama Yasuyuki Matsushita Kazuhiko Fukushima Yoshio Kijidani Keiji Takabe Kazufumi Yazaki Ichiro Kamei
author_sort	Natsumi Shimada
title	Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo
title_short	Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo
title_full	Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo
title_fullStr	Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo
title_full_unstemmed	Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo
title_sort	active transport of lignin precursors into membrane vesicles from lignifying tissues of bamboo
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/48f9b4e4c5c644cfa76ff4b8d3cfb1dd
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_version_	1718410768441933824

Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo

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