Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light

Qian Yang, Chuang Zhao, Jun Zhao, Yong Ye Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, People’s Republic of China Abstract: Cobra neurotoxin (CNT), a peptide isolated from snake venom of Naja naja...

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Autores principales:	Yang Q, Zhao C, Zhao J, Ye Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Cobra neurotoxin nanocapsules photo response central analgesia red light drug delivery Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/48fc3557d9754c5f83f00a42781976ce
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spelling	oai:doaj.org-article:48fc3557d9754c5f83f00a42781976ce2021-12-02T05:04:24ZPhotoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light1178-2013https://doaj.org/article/48fc3557d9754c5f83f00a42781976ce2017-05-01T00:00:00Zhttps://www.dovepress.com/photoresponsive-nanocapsulation-of-cobra-neurotoxin-and-enhancement-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qian Yang, Chuang Zhao, Jun Zhao, Yong Ye Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, People’s Republic of China Abstract: Cobra neurotoxin (CNT), a peptide isolated from snake venom of Naja naja atra, shows central analgesic effects in our previous research. In order to help CNT pass through blood–brain barrier (BBB) and improve its central analgesic effects, a new kind of CNT nanocapsules were prepared by double emulsification with soybean lecithin and cholesterol as the shell, and pheophorbide as the photosensitizer added to make it photoresponsive. The analgesic effects were evaluated by hot plate test and acetic acid-induced writhing in mice. The CNT nanocapsules had an average particle size of 229.55 nm, zeta potential of -53.00 mV, encapsulation efficiency of 84.81% and drug loading of 2.98%, when the pheophorbide content was 1% of lecithin weight. Pheophorbide was mainly distributed in outer layer of the CNT nanocapsules and increased the release of the CNT nanocapsules after 650 nm illumination. The central analgesic effects were improved after intraperitoneal injection of CNT at 25 and 50 µg·kg-1 under 650 nm irradiation for 30 min in the nasal cavity. Activation of pheophorbide by red light generated reactive oxygen species which opened the nanocapsules and BBB and helped the CNT enter the brain. This research provides a new drug delivery for treatment of central pain. Keywords: cobra neurotoxin, nanocapsules, photoresponsive, central analgesic effects, red light, drug delivery, photosensitizerYang QZhao CZhao JYe YDove Medical PressarticleCobra neurotoxinnanocapsulesphoto responsecentral analgesiared lightdrug deliveryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3463-3470 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cobra neurotoxin nanocapsules photo response central analgesia red light drug delivery Medicine (General) R5-920
spellingShingle	Cobra neurotoxin nanocapsules photo response central analgesia red light drug delivery Medicine (General) R5-920 Yang Q Zhao C Zhao J Ye Y Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
description	Qian Yang, Chuang Zhao, Jun Zhao, Yong Ye Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, People’s Republic of China Abstract: Cobra neurotoxin (CNT), a peptide isolated from snake venom of Naja naja atra, shows central analgesic effects in our previous research. In order to help CNT pass through blood–brain barrier (BBB) and improve its central analgesic effects, a new kind of CNT nanocapsules were prepared by double emulsification with soybean lecithin and cholesterol as the shell, and pheophorbide as the photosensitizer added to make it photoresponsive. The analgesic effects were evaluated by hot plate test and acetic acid-induced writhing in mice. The CNT nanocapsules had an average particle size of 229.55 nm, zeta potential of -53.00 mV, encapsulation efficiency of 84.81% and drug loading of 2.98%, when the pheophorbide content was 1% of lecithin weight. Pheophorbide was mainly distributed in outer layer of the CNT nanocapsules and increased the release of the CNT nanocapsules after 650 nm illumination. The central analgesic effects were improved after intraperitoneal injection of CNT at 25 and 50 µg·kg-1 under 650 nm irradiation for 30 min in the nasal cavity. Activation of pheophorbide by red light generated reactive oxygen species which opened the nanocapsules and BBB and helped the CNT enter the brain. This research provides a new drug delivery for treatment of central pain. Keywords: cobra neurotoxin, nanocapsules, photoresponsive, central analgesic effects, red light, drug delivery, photosensitizer
format	article
author	Yang Q Zhao C Zhao J Ye Y
author_facet	Yang Q Zhao C Zhao J Ye Y
author_sort	Yang Q
title	Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
title_short	Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
title_full	Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
title_fullStr	Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
title_full_unstemmed	Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
title_sort	photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/48fc3557d9754c5f83f00a42781976ce
work_keys_str_mv	AT yangq photoresponsivenanocapsulationofcobraneurotoxinandenhancementofitscentralanalgesiceffectsunderredlight AT zhaoc photoresponsivenanocapsulationofcobraneurotoxinandenhancementofitscentralanalgesiceffectsunderredlight AT zhaoj photoresponsivenanocapsulationofcobraneurotoxinandenhancementofitscentralanalgesiceffectsunderredlight AT yey photoresponsivenanocapsulationofcobraneurotoxinandenhancementofitscentralanalgesiceffectsunderredlight
_version_	1718400647206797312

Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light

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