Effects of Sulfur Assimilation in Pseudomonas fluorescens SS101 on Growth, Defense, and Metabolome of Different Brassicaceae

Genome-wide analysis of plant-growth-promoting Pseudomonas fluorescens strain SS101 (PfSS101) followed by site-directed mutagenesis previously suggested that sulfur assimilation may play an important role in growth promotion and induced systemic resistance in &l...

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Autores principales:	Je-Seung Jeon, Desalegn W. Etalo, Natalia Carreno-Quintero, Ric C. H. de Vos, Jos M. Raaijmakers
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	flavonoids glucosinolates (GLSs) induced systemic resistance <i>Pseudomonas fluorescens</i> plant growth promotion plant metabolomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/48fe326b4e604b4984cc3468bb7cab30
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spelling	oai:doaj.org-article:48fe326b4e604b4984cc3468bb7cab302021-11-25T16:53:59ZEffects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>10.3390/biom111117042218-273Xhttps://doaj.org/article/48fe326b4e604b4984cc3468bb7cab302021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1704https://doaj.org/toc/2218-273XGenome-wide analysis of plant-growth-promoting <i>Pseudomonas fluorescens</i> strain SS101 (<i>Pf</i>SS101) followed by site-directed mutagenesis previously suggested that sulfur assimilation may play an important role in growth promotion and induced systemic resistance in <i>Arabidopsis</i>. Here, we investigated the effects of sulfur metabolism in <i>Pf</i>SS101 on growth, defense, and shoot metabolomes of <i>Arabidopsis</i> and the Brassica crop, Broccoli. Root tips of seedlings of <i>Arabidopsis</i> and two Broccoli cultivars were treated with <i>Pf</i>SS101 or with a mutant disrupted in the adenylsulfate reductase <i>cysH</i>, a key gene in cysteine and methionine biosynthesis. Phenotyping of plants treated with wild-type <i>Pf</i>SS101 or its <i>cysH</i> mutant revealed that sulfur assimilation in <i>Pf</i>SS101 was associated with enhanced growth of <i>Arabidopsis</i> but with a reduction in shoot biomass of two Broccoli cultivars. Untargeted metabolomics revealed that <i>cysH</i>-mediated sulfur assimilation in <i>Pf</i>SS101 had significant effects on shoot chemistry of <i>Arabidopsis</i>, in particular on chain elongation of aliphatic glucosinolates (GLSs) and on indole metabolites, including camalexin and the growth hormone indole-3-acetic acid. In Broccoli, <i>Pf</i>SS101 sulfur assimilation significantly upregulated the relative abundance of several shoot metabolites, in particular, indolic GLSs and phenylpropanoids. These metabolome changes in Broccoli plants coincided with <i>Pf</i>SS101-mediated suppression of leaf infections by <i>Xanthomonas campestris</i>. Our study showed the metabolic interconnectedness of plants and their root-associated microbiota.Je-Seung JeonDesalegn W. EtaloNatalia Carreno-QuinteroRic C. H. de VosJos M. RaaijmakersMDPI AGarticleflavonoidsglucosinolates (GLSs)induced systemic resistance<i>Pseudomonas fluorescens</i>plant growth promotionplant metabolomicsMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1704, p 1704 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flavonoids glucosinolates (GLSs) induced systemic resistance <i>Pseudomonas fluorescens</i> plant growth promotion plant metabolomics Microbiology QR1-502
spellingShingle	flavonoids glucosinolates (GLSs) induced systemic resistance <i>Pseudomonas fluorescens</i> plant growth promotion plant metabolomics Microbiology QR1-502 Je-Seung Jeon Desalegn W. Etalo Natalia Carreno-Quintero Ric C. H. de Vos Jos M. Raaijmakers Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>
description	Genome-wide analysis of plant-growth-promoting <i>Pseudomonas fluorescens</i> strain SS101 (<i>Pf</i>SS101) followed by site-directed mutagenesis previously suggested that sulfur assimilation may play an important role in growth promotion and induced systemic resistance in <i>Arabidopsis</i>. Here, we investigated the effects of sulfur metabolism in <i>Pf</i>SS101 on growth, defense, and shoot metabolomes of <i>Arabidopsis</i> and the Brassica crop, Broccoli. Root tips of seedlings of <i>Arabidopsis</i> and two Broccoli cultivars were treated with <i>Pf</i>SS101 or with a mutant disrupted in the adenylsulfate reductase <i>cysH</i>, a key gene in cysteine and methionine biosynthesis. Phenotyping of plants treated with wild-type <i>Pf</i>SS101 or its <i>cysH</i> mutant revealed that sulfur assimilation in <i>Pf</i>SS101 was associated with enhanced growth of <i>Arabidopsis</i> but with a reduction in shoot biomass of two Broccoli cultivars. Untargeted metabolomics revealed that <i>cysH</i>-mediated sulfur assimilation in <i>Pf</i>SS101 had significant effects on shoot chemistry of <i>Arabidopsis</i>, in particular on chain elongation of aliphatic glucosinolates (GLSs) and on indole metabolites, including camalexin and the growth hormone indole-3-acetic acid. In Broccoli, <i>Pf</i>SS101 sulfur assimilation significantly upregulated the relative abundance of several shoot metabolites, in particular, indolic GLSs and phenylpropanoids. These metabolome changes in Broccoli plants coincided with <i>Pf</i>SS101-mediated suppression of leaf infections by <i>Xanthomonas campestris</i>. Our study showed the metabolic interconnectedness of plants and their root-associated microbiota.
format	article
author	Je-Seung Jeon Desalegn W. Etalo Natalia Carreno-Quintero Ric C. H. de Vos Jos M. Raaijmakers
author_facet	Je-Seung Jeon Desalegn W. Etalo Natalia Carreno-Quintero Ric C. H. de Vos Jos M. Raaijmakers
author_sort	Je-Seung Jeon
title	Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>
title_short	Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>
title_full	Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>
title_fullStr	Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>
title_full_unstemmed	Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>
title_sort	effects of sulfur assimilation in <i>pseudomonas fluorescens</i> ss101 on growth, defense, and metabolome of different <i>brassicaceae</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/48fe326b4e604b4984cc3468bb7cab30
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Effects of Sulfur Assimilation in <i>Pseudomonas fluorescens</i> SS101 on Growth, Defense, and Metabolome of Different <i>Brassicaceae</i>

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