Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes

Abstract The thioredoxin system plays a predominant role in the control of cellular redox status. Thioredoxin reductase fuels the system with reducing power in the form of NADPH. The TORC1 complex promotes growth and protein synthesis when nutrients, particularly amino acids, are abundant. It also r...

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Autores principales:	Cecilia Picazo, Emilia Matallana, Agustín Aranda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Chronological Life Span Grape Juice Fermentation TORC1 Pathway TORC1 Complex Thioredoxin System Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4902c81581aa48cfaeb4f234086bd61a
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spelling	oai:doaj.org-article:4902c81581aa48cfaeb4f234086bd61a2021-12-02T15:09:06ZYeast thioredoxin reductase Trr1p controls TORC1-regulated processes10.1038/s41598-018-34908-42045-2322https://doaj.org/article/4902c81581aa48cfaeb4f234086bd61a2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34908-4https://doaj.org/toc/2045-2322Abstract The thioredoxin system plays a predominant role in the control of cellular redox status. Thioredoxin reductase fuels the system with reducing power in the form of NADPH. The TORC1 complex promotes growth and protein synthesis when nutrients, particularly amino acids, are abundant. It also represses catabolic processes, like autophagy, which are activated during starvation. We analyzed the impact of yeast cytosolic thioredoxin reductase TRR1 deletion under different environmental conditions. It shortens chronological life span and reduces growth in grape juice fermentation. TRR1 deletion has a global impact on metabolism during fermentation. As expected, it reduces oxidative stress tolerance, but a compensatory response is triggered, with catalase and glutathione increasing. Unexpectedly, TRR1 deletion causes sensitivity to the inhibitors of the TORC1 pathway, such as rapamycin. This correlates with low Tor2p kinase levels and indicates a direct role of Trr1p in its stability. Markers of TORC1 activity, however, suggest increased TORC1 activity. The autophagy caused by nitrogen starvation is reduced in the trr1Δ mutant. Ribosomal protein Rsp6p is dephosphorylated in the presence of rapamycin. This dephosphorylation diminishes in the TRR1 deletion strain. These results show a complex network of interactions between thioredoxin reductase Trr1p and the processes controlled by TOR.Cecilia PicazoEmilia MatallanaAgustín ArandaNature PortfolioarticleChronological Life SpanGrape Juice FermentationTORC1 PathwayTORC1 ComplexThioredoxin SystemMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chronological Life Span Grape Juice Fermentation TORC1 Pathway TORC1 Complex Thioredoxin System Medicine R Science Q
spellingShingle	Chronological Life Span Grape Juice Fermentation TORC1 Pathway TORC1 Complex Thioredoxin System Medicine R Science Q Cecilia Picazo Emilia Matallana Agustín Aranda Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
description	Abstract The thioredoxin system plays a predominant role in the control of cellular redox status. Thioredoxin reductase fuels the system with reducing power in the form of NADPH. The TORC1 complex promotes growth and protein synthesis when nutrients, particularly amino acids, are abundant. It also represses catabolic processes, like autophagy, which are activated during starvation. We analyzed the impact of yeast cytosolic thioredoxin reductase TRR1 deletion under different environmental conditions. It shortens chronological life span and reduces growth in grape juice fermentation. TRR1 deletion has a global impact on metabolism during fermentation. As expected, it reduces oxidative stress tolerance, but a compensatory response is triggered, with catalase and glutathione increasing. Unexpectedly, TRR1 deletion causes sensitivity to the inhibitors of the TORC1 pathway, such as rapamycin. This correlates with low Tor2p kinase levels and indicates a direct role of Trr1p in its stability. Markers of TORC1 activity, however, suggest increased TORC1 activity. The autophagy caused by nitrogen starvation is reduced in the trr1Δ mutant. Ribosomal protein Rsp6p is dephosphorylated in the presence of rapamycin. This dephosphorylation diminishes in the TRR1 deletion strain. These results show a complex network of interactions between thioredoxin reductase Trr1p and the processes controlled by TOR.
format	article
author	Cecilia Picazo Emilia Matallana Agustín Aranda
author_facet	Cecilia Picazo Emilia Matallana Agustín Aranda
author_sort	Cecilia Picazo
title	Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
title_short	Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
title_full	Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
title_fullStr	Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
title_full_unstemmed	Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
title_sort	yeast thioredoxin reductase trr1p controls torc1-regulated processes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4902c81581aa48cfaeb4f234086bd61a
work_keys_str_mv	AT ceciliapicazo yeastthioredoxinreductasetrr1pcontrolstorc1regulatedprocesses AT emiliamatallana yeastthioredoxinreductasetrr1pcontrolstorc1regulatedprocesses AT agustinaranda yeastthioredoxinreductasetrr1pcontrolstorc1regulatedprocesses
_version_	1718387934902616064

Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes

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