Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times

The mulberry leaf is a classic herb commonly used in traditional Chinese medicine. It has also been used as animal feed for livestock and its fruits have been made into a variety of food products. Traditionally, mulberry (Morus alba L.) leaf harvesting after frost is thought to have better medicinal...

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Autores principales:	Ding-Qiao Xu, Shu-Yan Cheng, Jun-Qing Zhang, Han-Feng Lin, Yan-Yan Chen, Shi-Jun Yue, Meng Tian, Yu-Ping Tang, Yu-Cheng Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Morus alba L. transcriptome metabolomics harvest time flavonoid biosynthesis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/4904fe4a2dd540f8b7bdee5dd0f53858
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spelling	oai:doaj.org-article:4904fe4a2dd540f8b7bdee5dd0f538582021-11-16T15:40:55ZMorus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times1664-462X10.3389/fpls.2021.736332https://doaj.org/article/4904fe4a2dd540f8b7bdee5dd0f538582021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.736332/fullhttps://doaj.org/toc/1664-462XThe mulberry leaf is a classic herb commonly used in traditional Chinese medicine. It has also been used as animal feed for livestock and its fruits have been made into a variety of food products. Traditionally, mulberry (Morus alba L.) leaf harvesting after frost is thought to have better medicinal properties, but the underlying mechanism remains largely unsolved. To elucidate the biological basis of mulberry leaves after frost, we first explored the content changes of various compounds in mulberry leaves at different harvest times. Significant enrichment of flavonoids was observed with a total of 224 differential metabolites after frost. Subsequently, we analyzed the transcriptomic data of mulberry leaves collected at different harvest times and successfully annotated 22,939 unigenes containing 1,695 new genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed 26, 20, and 59 unigenes related to flavonoids synthesis in three different groups harvested at different times. We found that the expression levels of flavonoid biosynthesis-related unigenes also increased when harvested at a delayed time, which was consistent with the flavonoid accumulation discovered by the metabolomic analysis. The results indicated that low temperature may be a key trigger in flavonoid biosynthesis of mulberry leaves by increasing the expression of flavonoid biosynthesis-related genes. This study also provided a theoretical basis for the optimal harvest time of mulberry leaves.Ding-Qiao XuShu-Yan ChengJun-Qing ZhangHan-Feng LinYan-Yan ChenShi-Jun YueMeng TianYu-Ping TangYu-Cheng ZhaoFrontiers Media S.A.articleMorus alba L.transcriptomemetabolomicsharvest timeflavonoid biosynthesisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Morus alba L. transcriptome metabolomics harvest time flavonoid biosynthesis Plant culture SB1-1110
spellingShingle	Morus alba L. transcriptome metabolomics harvest time flavonoid biosynthesis Plant culture SB1-1110 Ding-Qiao Xu Shu-Yan Cheng Jun-Qing Zhang Han-Feng Lin Yan-Yan Chen Shi-Jun Yue Meng Tian Yu-Ping Tang Yu-Cheng Zhao Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times
description	The mulberry leaf is a classic herb commonly used in traditional Chinese medicine. It has also been used as animal feed for livestock and its fruits have been made into a variety of food products. Traditionally, mulberry (Morus alba L.) leaf harvesting after frost is thought to have better medicinal properties, but the underlying mechanism remains largely unsolved. To elucidate the biological basis of mulberry leaves after frost, we first explored the content changes of various compounds in mulberry leaves at different harvest times. Significant enrichment of flavonoids was observed with a total of 224 differential metabolites after frost. Subsequently, we analyzed the transcriptomic data of mulberry leaves collected at different harvest times and successfully annotated 22,939 unigenes containing 1,695 new genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed 26, 20, and 59 unigenes related to flavonoids synthesis in three different groups harvested at different times. We found that the expression levels of flavonoid biosynthesis-related unigenes also increased when harvested at a delayed time, which was consistent with the flavonoid accumulation discovered by the metabolomic analysis. The results indicated that low temperature may be a key trigger in flavonoid biosynthesis of mulberry leaves by increasing the expression of flavonoid biosynthesis-related genes. This study also provided a theoretical basis for the optimal harvest time of mulberry leaves.
format	article
author	Ding-Qiao Xu Shu-Yan Cheng Jun-Qing Zhang Han-Feng Lin Yan-Yan Chen Shi-Jun Yue Meng Tian Yu-Ping Tang Yu-Cheng Zhao
author_facet	Ding-Qiao Xu Shu-Yan Cheng Jun-Qing Zhang Han-Feng Lin Yan-Yan Chen Shi-Jun Yue Meng Tian Yu-Ping Tang Yu-Cheng Zhao
author_sort	Ding-Qiao Xu
title	Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times
title_short	Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times
title_full	Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times
title_fullStr	Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times
title_full_unstemmed	Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times
title_sort	morus alba l. leaves – integration of their transcriptome and metabolomics dataset: investigating potential genes involved in flavonoid biosynthesis at different harvest times
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4904fe4a2dd540f8b7bdee5dd0f53858
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Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times

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