High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells

Riboswitches can mediate ligand-dependent RNA cleavage and splicing to control gene expression. Here the authors present a method to functionally screen large libraries and identify functional variants.

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Autores principales:	Benjamin Strobel, Maike Spöring, Holger Klein, Dragica Blazevic, Werner Rust, Sergi Sayols, Jörg S. Hartig, Sebastian Kreuz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/490841ff4d214574955fa93d388d9d36
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spelling	oai:doaj.org-article:490841ff4d214574955fa93d388d9d362021-12-02T17:32:12ZHigh-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells10.1038/s41467-020-14491-x2041-1723https://doaj.org/article/490841ff4d214574955fa93d388d9d362020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14491-xhttps://doaj.org/toc/2041-1723Riboswitches can mediate ligand-dependent RNA cleavage and splicing to control gene expression. Here the authors present a method to functionally screen large libraries and identify functional variants.Benjamin StrobelMaike SpöringHolger KleinDragica BlazevicWerner RustSergi SayolsJörg S. HartigSebastian KreuzNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Benjamin Strobel Maike Spöring Holger Klein Dragica Blazevic Werner Rust Sergi Sayols Jörg S. Hartig Sebastian Kreuz High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
description	Riboswitches can mediate ligand-dependent RNA cleavage and splicing to control gene expression. Here the authors present a method to functionally screen large libraries and identify functional variants.
format	article
author	Benjamin Strobel Maike Spöring Holger Klein Dragica Blazevic Werner Rust Sergi Sayols Jörg S. Hartig Sebastian Kreuz
author_facet	Benjamin Strobel Maike Spöring Holger Klein Dragica Blazevic Werner Rust Sergi Sayols Jörg S. Hartig Sebastian Kreuz
author_sort	Benjamin Strobel
title	High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
title_short	High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
title_full	High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
title_fullStr	High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
title_full_unstemmed	High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
title_sort	high-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/490841ff4d214574955fa93d388d9d36
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High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells

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