Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens

Abstract Extensive rodent literature suggests that the endocannabinoid (eCB) system present in the nucleus accumbens (NAc) modulates dopamine (DA) release in this area. However, expression patterns of the cannabinoid receptor type 1 (CB1R), the synthesizing enzyme N-acyl phosphatidylethanolamine pho...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ryan Kucera, Joseph Bouskila, Laurent Elkrief, Anders Fink-Jensen, Roberta Palmour, Jean-François Bouchard, Maurice Ptito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/490d0a9ff99643f9b53ba07f6b3acbf9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:490d0a9ff99643f9b53ba07f6b3acbf9
record_format	dspace
spelling	oai:doaj.org-article:490d0a9ff99643f9b53ba07f6b3acbf92021-12-02T15:07:47ZExpression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens10.1038/s41598-018-26826-22045-2322https://doaj.org/article/490d0a9ff99643f9b53ba07f6b3acbf92018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26826-2https://doaj.org/toc/2045-2322Abstract Extensive rodent literature suggests that the endocannabinoid (eCB) system present in the nucleus accumbens (NAc) modulates dopamine (DA) release in this area. However, expression patterns of the cannabinoid receptor type 1 (CB1R), the synthesizing enzyme N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD), and the degradation enzyme fatty acid amide hydrolase (FAAH) in the NAc have not yet been described in non-human primates. The goal of this study is therefore to characterize the expression and localization of the eCB system within the NAc of vervet monkeys (Chlorocebus sabaeus) using Western blots and immunohistochemistry. Results show that CB1R, NAPE-PLD, and FAAH are expressed across the NAc rostrocaudal axis, both in the core and shell. CB1R, NAPE-PLD, and FAAH are localized in medium spiny neurons (MSNs) and fast-spiking GABAergic interneurons (FSIs). Dopaminergic projections and astrocytes did not express CB1R, NAPE-PLD, or FAAH. These data show that the eCB system is present in the vervet monkey NAc and supports its role in the primate brain reward circuit.Ryan KuceraJoseph BouskilaLaurent ElkriefAnders Fink-JensenRoberta PalmourJean-François BouchardMaurice PtitoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan Kucera Joseph Bouskila Laurent Elkrief Anders Fink-Jensen Roberta Palmour Jean-François Bouchard Maurice Ptito Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
description	Abstract Extensive rodent literature suggests that the endocannabinoid (eCB) system present in the nucleus accumbens (NAc) modulates dopamine (DA) release in this area. However, expression patterns of the cannabinoid receptor type 1 (CB1R), the synthesizing enzyme N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD), and the degradation enzyme fatty acid amide hydrolase (FAAH) in the NAc have not yet been described in non-human primates. The goal of this study is therefore to characterize the expression and localization of the eCB system within the NAc of vervet monkeys (Chlorocebus sabaeus) using Western blots and immunohistochemistry. Results show that CB1R, NAPE-PLD, and FAAH are expressed across the NAc rostrocaudal axis, both in the core and shell. CB1R, NAPE-PLD, and FAAH are localized in medium spiny neurons (MSNs) and fast-spiking GABAergic interneurons (FSIs). Dopaminergic projections and astrocytes did not express CB1R, NAPE-PLD, or FAAH. These data show that the eCB system is present in the vervet monkey NAc and supports its role in the primate brain reward circuit.
format	article
author	Ryan Kucera Joseph Bouskila Laurent Elkrief Anders Fink-Jensen Roberta Palmour Jean-François Bouchard Maurice Ptito
author_facet	Ryan Kucera Joseph Bouskila Laurent Elkrief Anders Fink-Jensen Roberta Palmour Jean-François Bouchard Maurice Ptito
author_sort	Ryan Kucera
title	Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
title_short	Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
title_full	Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
title_fullStr	Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
title_full_unstemmed	Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
title_sort	expression and localization of cb1r, nape-pld, and faah in the vervet monkey nucleus accumbens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/490d0a9ff99643f9b53ba07f6b3acbf9
work_keys_str_mv	AT ryankucera expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens AT josephbouskila expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens AT laurentelkrief expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens AT andersfinkjensen expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens AT robertapalmour expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens AT jeanfrancoisbouchard expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens AT mauriceptito expressionandlocalizationofcb1rnapepldandfaahinthevervetmonkeynucleusaccumbens
_version_	1718388381393616896

Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens

Ejemplares similares