Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line

Hexagonal zinc oxide nanorods (ZnO-NRs) were prepared via solution process using zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 0.25 M), hexamethylenetetramine (HMT) and sodium hydroxide at low concentration (4 × 10-3 M in 100 ml of distilled water) and refluxed at 100 °C for an hour. The HMT was used bec...

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Autores principales:	Javed Ahmad, Rizwan Wahab, Mohd Javed Akhtar, Maqusood Ahamed
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Zinc oxide nanorods X-ray diffraction pattern FTIR spectroscopy Human cell line ROS RT-PCR Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/490d4110a90049d19a093368a861be09
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spelling	oai:doaj.org-article:490d4110a90049d19a093368a861be092021-11-18T04:44:28ZCytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line1018-364710.1016/j.jksus.2021.101658https://doaj.org/article/490d4110a90049d19a093368a861be092021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003207https://doaj.org/toc/1018-3647Hexagonal zinc oxide nanorods (ZnO-NRs) were prepared via solution process using zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 0.25 M), hexamethylenetetramine (HMT) and sodium hydroxide at low concentration (4 × 10-3 M in 100 ml of distilled water) and refluxed at 100 °C for an hour. The HMT was used because its act as a template for the nucleation and growth of zinc oxide nanorods. The X-ray diffraction patterns (XRD) clearly reveal that the grown product is pure zinc oxide. The diameters and lengths of the synthesized nanorods lie between 200 and 250 nm and 2–3 μm respectively as observed from the field emission scanning electron microscopy (FESEM). The chemical functional properties was analyzed by Fourier Transform infra-red (FTIR) spectroscopy. This study was designed to show the possible effect of ZnO-NRs in human liver cancer (HepG2) cells. It induces cytotoxicity via reactive oxygen species (ROS), mitochondrial membrane potential. For gene expression analysis, it induces apoptotic gene marker P53, Bax, caspase3, Bcl2 genes. Based on analysis and observations, it concludes that the ZnO-NRs were utilized for toxicity at dose dependent via ROS generation against human hepato cellular liver carcinoma cell line.Javed AhmadRizwan WahabMohd Javed AkhtarMaqusood AhamedElsevierarticleZinc oxide nanorodsX-ray diffraction patternFTIR spectroscopyHuman cell lineROSRT-PCRScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101658- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zinc oxide nanorods X-ray diffraction pattern FTIR spectroscopy Human cell line ROS RT-PCR Science (General) Q1-390
spellingShingle	Zinc oxide nanorods X-ray diffraction pattern FTIR spectroscopy Human cell line ROS RT-PCR Science (General) Q1-390 Javed Ahmad Rizwan Wahab Mohd Javed Akhtar Maqusood Ahamed Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
description	Hexagonal zinc oxide nanorods (ZnO-NRs) were prepared via solution process using zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 0.25 M), hexamethylenetetramine (HMT) and sodium hydroxide at low concentration (4 × 10-3 M in 100 ml of distilled water) and refluxed at 100 °C for an hour. The HMT was used because its act as a template for the nucleation and growth of zinc oxide nanorods. The X-ray diffraction patterns (XRD) clearly reveal that the grown product is pure zinc oxide. The diameters and lengths of the synthesized nanorods lie between 200 and 250 nm and 2–3 μm respectively as observed from the field emission scanning electron microscopy (FESEM). The chemical functional properties was analyzed by Fourier Transform infra-red (FTIR) spectroscopy. This study was designed to show the possible effect of ZnO-NRs in human liver cancer (HepG2) cells. It induces cytotoxicity via reactive oxygen species (ROS), mitochondrial membrane potential. For gene expression analysis, it induces apoptotic gene marker P53, Bax, caspase3, Bcl2 genes. Based on analysis and observations, it concludes that the ZnO-NRs were utilized for toxicity at dose dependent via ROS generation against human hepato cellular liver carcinoma cell line.
format	article
author	Javed Ahmad Rizwan Wahab Mohd Javed Akhtar Maqusood Ahamed
author_facet	Javed Ahmad Rizwan Wahab Mohd Javed Akhtar Maqusood Ahamed
author_sort	Javed Ahmad
title	Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
title_short	Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
title_full	Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
title_fullStr	Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
title_full_unstemmed	Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
title_sort	cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/490d4110a90049d19a093368a861be09
work_keys_str_mv	AT javedahmad cytotoxicityandapoptosisresponseofhexagonalzincoxidenanorodsagainsthumanhepatocellularlivercarcinomacellline AT rizwanwahab cytotoxicityandapoptosisresponseofhexagonalzincoxidenanorodsagainsthumanhepatocellularlivercarcinomacellline AT mohdjavedakhtar cytotoxicityandapoptosisresponseofhexagonalzincoxidenanorodsagainsthumanhepatocellularlivercarcinomacellline AT maqusoodahamed cytotoxicityandapoptosisresponseofhexagonalzincoxidenanorodsagainsthumanhepatocellularlivercarcinomacellline
_version_	1718425097436397568

Cytotoxicity and apoptosis response of hexagonal zinc oxide nanorods against human hepatocellular liver carcinoma cell line

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