Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present st...

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Autores principales:	Laura Preckel, Claudia Brünen-Nieweler, Grégoire Denay, Henning Petersen, Margit Cichna-Markl, Stefanie Dobrovolny, Rupert Hochegger
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	DNA metabarcoding 16S rDNA meat species identification authentication food pet food Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/4910bbc3efeb4cfd851dcd7d0881fa1f
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id	oai:doaj.org-article:4910bbc3efeb4cfd851dcd7d0881fa1f
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spelling	oai:doaj.org-article:4910bbc3efeb4cfd851dcd7d0881fa1f2021-11-25T17:36:48ZIdentification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding10.3390/foods101128752304-8158https://doaj.org/article/4910bbc3efeb4cfd851dcd7d0881fa1f2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2875https://doaj.org/toc/2304-8158The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species.Laura PreckelClaudia Brünen-NiewelerGrégoire DenayHenning PetersenMargit Cichna-MarklStefanie DobrovolnyRupert HocheggerMDPI AGarticleDNA metabarcoding16S rDNAmeat species identificationauthenticationfoodpet foodChemical technologyTP1-1185ENFoods, Vol 10, Iss 2875, p 2875 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA metabarcoding 16S rDNA meat species identification authentication food pet food Chemical technology TP1-1185
spellingShingle	DNA metabarcoding 16S rDNA meat species identification authentication food pet food Chemical technology TP1-1185 Laura Preckel Claudia Brünen-Nieweler Grégoire Denay Henning Petersen Margit Cichna-Markl Stefanie Dobrovolny Rupert Hochegger Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
description	The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species.
format	article
author	Laura Preckel Claudia Brünen-Nieweler Grégoire Denay Henning Petersen Margit Cichna-Markl Stefanie Dobrovolny Rupert Hochegger
author_facet	Laura Preckel Claudia Brünen-Nieweler Grégoire Denay Henning Petersen Margit Cichna-Markl Stefanie Dobrovolny Rupert Hochegger
author_sort	Laura Preckel
title	Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
title_short	Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
title_full	Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
title_fullStr	Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
title_full_unstemmed	Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding
title_sort	identification of mammalian and poultry species in food and pet food samples using 16s rdna metabarcoding
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4910bbc3efeb4cfd851dcd7d0881fa1f
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Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

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