Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato

Abstract. Effendy J, Labonte DR, Efendi D. 2019. Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato. Biodiversitas 20: 3033-3041. Three cDNAs (IbH23, IbH41, and IbH74) encoding sporamin of sweet potato, were isolated from a skinning injury cDNA librar...

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Autores principales:	Jollanda Effendy, Don R. LaBonte, Darda Efendi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	cysteine residues, ipomoea batatas, miraculin, storage root, wounding Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/491302d873bd44078ec3a41a3e889b38
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spelling	oai:doaj.org-article:491302d873bd44078ec3a41a3e889b382021-11-21T21:54:57ZIsolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato1412-033X2085-472210.13057/biodiv/d201036https://doaj.org/article/491302d873bd44078ec3a41a3e889b382019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4303https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Effendy J, Labonte DR, Efendi D. 2019. Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato. Biodiversitas 20: 3033-3041. Three cDNAs (IbH23, IbH41, and IbH74) encoding sporamin of sweet potato, were isolated from a skinning injury cDNA library. The open reading frame consisted of 288 nucleotides and the deduced polypeptide sequences consist of 96 amino acids with missing 127 amino acid residues at 5?. The sequences shared identity [42-62% (IbH23 and IbH41) and 40-61% (IbH74)] to sporamins A and B previously identified in other sweet potato species. The IbSpors contained three of four signature cysteine residues of sporamin family (Cys135, Cys198, Cys209). In addition to similarity to sporamins, these cDNA clones also showed similarities to miraculin (29-35%); Kunitz trypsin inhibitor (29-36%); factor Xa inhibitor (32-38%); subtilisin inhibitor (26-37%); seed protein (32%); tumor-related protein (36% in IbH23 and IbH41); and [wound-responsive protein (30%), sexual organ responsive protein (29%), and proteinase inhibitor (26%) can only be found in IbH74]. The three partial mRNA transcripts showed induced and transient expression in response to skinning injury. The phylogenetic analysis revealed that they belonged to a new member of sporamin gene subfamily B and may play key roles in plant defense and wound repair in response to skinning injury in storage roots of sweet potato.Jollanda EffendyDon R. LaBonteDarda EfendiMBI & UNS Soloarticlecysteine residues, ipomoea batatas, miraculin, storage root, woundingBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cysteine residues, ipomoea batatas, miraculin, storage root, wounding Biology (General) QH301-705.5
spellingShingle	cysteine residues, ipomoea batatas, miraculin, storage root, wounding Biology (General) QH301-705.5 Jollanda Effendy Don R. LaBonte Darda Efendi Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato
description	Abstract. Effendy J, Labonte DR, Efendi D. 2019. Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato. Biodiversitas 20: 3033-3041. Three cDNAs (IbH23, IbH41, and IbH74) encoding sporamin of sweet potato, were isolated from a skinning injury cDNA library. The open reading frame consisted of 288 nucleotides and the deduced polypeptide sequences consist of 96 amino acids with missing 127 amino acid residues at 5?. The sequences shared identity [42-62% (IbH23 and IbH41) and 40-61% (IbH74)] to sporamins A and B previously identified in other sweet potato species. The IbSpors contained three of four signature cysteine residues of sporamin family (Cys135, Cys198, Cys209). In addition to similarity to sporamins, these cDNA clones also showed similarities to miraculin (29-35%); Kunitz trypsin inhibitor (29-36%); factor Xa inhibitor (32-38%); subtilisin inhibitor (26-37%); seed protein (32%); tumor-related protein (36% in IbH23 and IbH41); and [wound-responsive protein (30%), sexual organ responsive protein (29%), and proteinase inhibitor (26%) can only be found in IbH74]. The three partial mRNA transcripts showed induced and transient expression in response to skinning injury. The phylogenetic analysis revealed that they belonged to a new member of sporamin gene subfamily B and may play key roles in plant defense and wound repair in response to skinning injury in storage roots of sweet potato.
format	article
author	Jollanda Effendy Don R. LaBonte Darda Efendi
author_facet	Jollanda Effendy Don R. LaBonte Darda Efendi
author_sort	Jollanda Effendy
title	Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato
title_short	Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato
title_full	Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato
title_fullStr	Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato
title_full_unstemmed	Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato
title_sort	isolation and characterization of cdna clones encoding a novel subfamily sporamin b in sweet potato
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/491302d873bd44078ec3a41a3e889b38
work_keys_str_mv	AT jollandaeffendy isolationandcharacterizationofcdnaclonesencodinganovelsubfamilysporaminbinsweetpotato AT donrlabonte isolationandcharacterizationofcdnaclonesencodinganovelsubfamilysporaminbinsweetpotato AT dardaefendi isolationandcharacterizationofcdnaclonesencodinganovelsubfamilysporaminbinsweetpotato
_version_	1718418638695825408

Isolation and characterization of cDNA clones encoding a novel subfamily sporamin B in sweet potato

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