Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold

The aim of the research was to study of the therapeutic efficacy of combined transplantation of liver cells and mesenchymal stem cells of bone marrow (MSC) on TiNi-based alloy scaffolds of rats with chronic hepatitis. Materials and methods: Three groups of experiments were carried out on Wistar rats...

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Autores principales:	O. V. Kokorev, S. V. Gyunter, V. N. Khodorenko, G. T. Dambaev
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	tissue engineering liver failure cell transplantation cell-engineering design scaffold porous tini-based alloy Science Q
Acceso en línea:	https://doaj.org/article/491f49399f744a2dad550d37da19c71d
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spelling	oai:doaj.org-article:491f49399f744a2dad550d37da19c71d2021-11-23T06:14:40ZCombined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold2541-94202587-959610.29413/ABS.2018-3.3.30https://doaj.org/article/491f49399f744a2dad550d37da19c71d2018-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/607https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The aim of the research was to study of the therapeutic efficacy of combined transplantation of liver cells and mesenchymal stem cells of bone marrow (MSC) on TiNi-based alloy scaffolds of rats with chronic hepatitis. Materials and methods: Three groups of experiments were carried out on Wistar rats and intact rats (control) - I group; in the II group - chronic hepatitis; III group - cell-engineering designs with liver cells; in the IV group - cell-engineering designs with liver cells and BM MMSC. The activity of recovery processes was evaluated by using biochemical methods in dynamics on 10, 20, 30th days. Results. The optimal ratio of liver cells to MSC (5:1) was previously found in TiNi-based alloy scaffolds after transplantation and through co-cultivation of hepatocytes and MSC at different ratios. It was shown that recovery processes in the group with scaffolds transplanted into damaged livers were much more active if compared with those in the control group with chronic hepatitis. At the same time, in the group with combined transplantation on TiNi-based alloy scaffolds contributed to a more rapid normalization of liver enzyme indices compared with the group with transplantation of some hepatocytes on scaffolds. These data are confirmed by an increase in the life span of animals with combined hepatocytes and MSC transplantation on TiNi-based alloy scaffolds. Conclusions. A higher level and prolonged periods of liver regeneration during the transplantation of TiNi-based alloy scaffolds are due to the creation of biologically appropriate conditions for cells with certain ratio.O. V. KokorevS. V. GyunterV. N. KhodorenkoG. T. DambaevScientific Сentre for Family Health and Human Reproduction Problemsarticletissue engineeringliver failurecell transplantationcell-engineering designscaffoldporous tini-based alloyScienceQRUActa Biomedica Scientifica, Vol 3, Iss 3, Pp 195-203 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	tissue engineering liver failure cell transplantation cell-engineering design scaffold porous tini-based alloy Science Q
spellingShingle	tissue engineering liver failure cell transplantation cell-engineering design scaffold porous tini-based alloy Science Q O. V. Kokorev S. V. Gyunter V. N. Khodorenko G. T. Dambaev Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold
description	The aim of the research was to study of the therapeutic efficacy of combined transplantation of liver cells and mesenchymal stem cells of bone marrow (MSC) on TiNi-based alloy scaffolds of rats with chronic hepatitis. Materials and methods: Three groups of experiments were carried out on Wistar rats and intact rats (control) - I group; in the II group - chronic hepatitis; III group - cell-engineering designs with liver cells; in the IV group - cell-engineering designs with liver cells and BM MMSC. The activity of recovery processes was evaluated by using biochemical methods in dynamics on 10, 20, 30th days. Results. The optimal ratio of liver cells to MSC (5:1) was previously found in TiNi-based alloy scaffolds after transplantation and through co-cultivation of hepatocytes and MSC at different ratios. It was shown that recovery processes in the group with scaffolds transplanted into damaged livers were much more active if compared with those in the control group with chronic hepatitis. At the same time, in the group with combined transplantation on TiNi-based alloy scaffolds contributed to a more rapid normalization of liver enzyme indices compared with the group with transplantation of some hepatocytes on scaffolds. These data are confirmed by an increase in the life span of animals with combined hepatocytes and MSC transplantation on TiNi-based alloy scaffolds. Conclusions. A higher level and prolonged periods of liver regeneration during the transplantation of TiNi-based alloy scaffolds are due to the creation of biologically appropriate conditions for cells with certain ratio.
format	article
author	O. V. Kokorev S. V. Gyunter V. N. Khodorenko G. T. Dambaev
author_facet	O. V. Kokorev S. V. Gyunter V. N. Khodorenko G. T. Dambaev
author_sort	O. V. Kokorev
title	Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold
title_short	Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold
title_full	Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold
title_fullStr	Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold
title_full_unstemmed	Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold
title_sort	combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable tini-based alloy scaffold
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/491f49399f744a2dad550d37da19c71d
work_keys_str_mv	AT ovkokorev combinedtransplantationofhepatocyteswithmesenchimalstemcellsofbonemarrowonporouspermeabletinibasedalloyscaffold AT svgyunter combinedtransplantationofhepatocyteswithmesenchimalstemcellsofbonemarrowonporouspermeabletinibasedalloyscaffold AT vnkhodorenko combinedtransplantationofhepatocyteswithmesenchimalstemcellsofbonemarrowonporouspermeabletinibasedalloyscaffold AT gtdambaev combinedtransplantationofhepatocyteswithmesenchimalstemcellsofbonemarrowonporouspermeabletinibasedalloyscaffold
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Combined transplantation of hepatocytes with mesenchimal stem cells of bone marrow on porous-permeable TiNi-based alloy scaffold

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