Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models

Abstract Liver extracellular matrix (ECM)-based hydrogels have gained considerable interest as biomimetic 3D cell culture environments to investigate the mechanisms of liver pathology, metabolism, and toxicity. The preparation of current liver ECM hydrogels, however, is based on time-consuming therm...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Akhilandeshwari Ravichandran, Berline Murekatete, Denise Moedder, Christoph Meinert, Laura J. Bray
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/491fa025fe4b4c02beea3ed0e7195ee5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:491fa025fe4b4c02beea3ed0e7195ee5
record_format	dspace
spelling	oai:doaj.org-article:491fa025fe4b4c02beea3ed0e7195ee52021-12-02T16:31:51ZPhotocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models10.1038/s41598-021-94990-z2045-2322https://doaj.org/article/491fa025fe4b4c02beea3ed0e7195ee52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94990-zhttps://doaj.org/toc/2045-2322Abstract Liver extracellular matrix (ECM)-based hydrogels have gained considerable interest as biomimetic 3D cell culture environments to investigate the mechanisms of liver pathology, metabolism, and toxicity. The preparation of current liver ECM hydrogels, however, is based on time-consuming thermal gelation and limits the control of mechanical properties. In this study, we used detergent-based protocols to produce decellularized porcine liver ECM, which in turn were solubilized and functionalized with methacrylic anhydride to generate photocrosslinkable methacrylated liver ECM (LivMA) hydrogels. Firstly, we explored the efficacy of two protocols to decellularize porcine liver tissue using varying combinations of commonly used chemical agents such as Triton X-100, Sodium Dodecyl Sulphate (SDS) and Ammonium hydroxide. Then, we demonstrated successful formation of stable, reproducible LivMA hydrogels from both the protocols by photocrosslinking. The LivMA hydrogels obtained from the two decellularization protocols showed distinct mechanical properties. The compressive modulus of the hydrogels was directly dependent on the hydrogel concentration, thereby demonstrating the tuneability of mechanical properties of these hydrogels. Immortalized Human Hepatocytes cells were encapsulated in the LivMA hydrogels and cytocompatibility of the hydrogels was demonstrated after one week of culture. In summary, the LivMA hydrogel system provides a simple, photocrosslinkable platform, which can potentially be used to simulate healthy versus damaged liver for liver disease research, drug studies and cancer metastasis modelling.Akhilandeshwari RavichandranBerline MurekateteDenise MoedderChristoph MeinertLaura J. BrayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akhilandeshwari Ravichandran Berline Murekatete Denise Moedder Christoph Meinert Laura J. Bray Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models
description	Abstract Liver extracellular matrix (ECM)-based hydrogels have gained considerable interest as biomimetic 3D cell culture environments to investigate the mechanisms of liver pathology, metabolism, and toxicity. The preparation of current liver ECM hydrogels, however, is based on time-consuming thermal gelation and limits the control of mechanical properties. In this study, we used detergent-based protocols to produce decellularized porcine liver ECM, which in turn were solubilized and functionalized with methacrylic anhydride to generate photocrosslinkable methacrylated liver ECM (LivMA) hydrogels. Firstly, we explored the efficacy of two protocols to decellularize porcine liver tissue using varying combinations of commonly used chemical agents such as Triton X-100, Sodium Dodecyl Sulphate (SDS) and Ammonium hydroxide. Then, we demonstrated successful formation of stable, reproducible LivMA hydrogels from both the protocols by photocrosslinking. The LivMA hydrogels obtained from the two decellularization protocols showed distinct mechanical properties. The compressive modulus of the hydrogels was directly dependent on the hydrogel concentration, thereby demonstrating the tuneability of mechanical properties of these hydrogels. Immortalized Human Hepatocytes cells were encapsulated in the LivMA hydrogels and cytocompatibility of the hydrogels was demonstrated after one week of culture. In summary, the LivMA hydrogel system provides a simple, photocrosslinkable platform, which can potentially be used to simulate healthy versus damaged liver for liver disease research, drug studies and cancer metastasis modelling.
format	article
author	Akhilandeshwari Ravichandran Berline Murekatete Denise Moedder Christoph Meinert Laura J. Bray
author_facet	Akhilandeshwari Ravichandran Berline Murekatete Denise Moedder Christoph Meinert Laura J. Bray
author_sort	Akhilandeshwari Ravichandran
title	Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models
title_short	Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models
title_full	Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models
title_fullStr	Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models
title_full_unstemmed	Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models
title_sort	photocrosslinkable liver extracellular matrix hydrogels for the generation of 3d liver microenvironment models
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/491fa025fe4b4c02beea3ed0e7195ee5
work_keys_str_mv	AT akhilandeshwariravichandran photocrosslinkableliverextracellularmatrixhydrogelsforthegenerationof3dlivermicroenvironmentmodels AT berlinemurekatete photocrosslinkableliverextracellularmatrixhydrogelsforthegenerationof3dlivermicroenvironmentmodels AT denisemoedder photocrosslinkableliverextracellularmatrixhydrogelsforthegenerationof3dlivermicroenvironmentmodels AT christophmeinert photocrosslinkableliverextracellularmatrixhydrogelsforthegenerationof3dlivermicroenvironmentmodels AT laurajbray photocrosslinkableliverextracellularmatrixhydrogelsforthegenerationof3dlivermicroenvironmentmodels
_version_	1718383807451627520

Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models

Ejemplares similares