Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.

The heat shock response (HSR) is a highly conserved cellular process that promotes survival during stress. A hallmark of the HSR is the rapid induction of heat shock proteins (HSPs), such as HSP-70, by transcriptional activation. Once the stress is alleviated, HSPs return to near basal levels throug...

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Autores principales:	Delaney C Pagliuso, Devavrat M Bodas, Amy E Pasquinelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/4925b37160fc4debb8af21e5912cd790
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spelling	oai:doaj.org-article:4925b37160fc4debb8af21e5912cd7902021-12-02T20:02:53ZRecovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.1553-73901553-740410.1371/journal.pgen.1009734https://doaj.org/article/4925b37160fc4debb8af21e5912cd7902021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009734https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The heat shock response (HSR) is a highly conserved cellular process that promotes survival during stress. A hallmark of the HSR is the rapid induction of heat shock proteins (HSPs), such as HSP-70, by transcriptional activation. Once the stress is alleviated, HSPs return to near basal levels through incompletely understood mechanisms. Here, we show that the microRNA pathway acts during heat shock recovery in Caenorhabditis elegans. Depletion of the miRNA Argonaute, Argonaute Like Gene 1 (ALG-1), after an episode of heat shock resulted in decreased survival and perdurance of high hsp-70 levels. We present evidence that regulation of hsp-70 is dependent on miR-85 and sequences in the hsp-70 3'UTR that contain target sites for this miRNA. Regulation of hsp-70 by the miRNA pathway was found to be particularly important during recovery from HS, as animals that lacked miR-85 or its target sites in the hsp-70 3'UTR overexpressed HSP-70 and exhibited reduced viability. In summary, our findings show that down-regulation of hsp-70 by miR-85 after HS promotes survival, highlighting a previously unappreciated role for the miRNA pathway during recovery from stress.Delaney C PagliusoDevavrat M BodasAmy E PasquinelliPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009734 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Delaney C Pagliuso Devavrat M Bodas Amy E Pasquinelli Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.
description	The heat shock response (HSR) is a highly conserved cellular process that promotes survival during stress. A hallmark of the HSR is the rapid induction of heat shock proteins (HSPs), such as HSP-70, by transcriptional activation. Once the stress is alleviated, HSPs return to near basal levels through incompletely understood mechanisms. Here, we show that the microRNA pathway acts during heat shock recovery in Caenorhabditis elegans. Depletion of the miRNA Argonaute, Argonaute Like Gene 1 (ALG-1), after an episode of heat shock resulted in decreased survival and perdurance of high hsp-70 levels. We present evidence that regulation of hsp-70 is dependent on miR-85 and sequences in the hsp-70 3'UTR that contain target sites for this miRNA. Regulation of hsp-70 by the miRNA pathway was found to be particularly important during recovery from HS, as animals that lacked miR-85 or its target sites in the hsp-70 3'UTR overexpressed HSP-70 and exhibited reduced viability. In summary, our findings show that down-regulation of hsp-70 by miR-85 after HS promotes survival, highlighting a previously unappreciated role for the miRNA pathway during recovery from stress.
format	article
author	Delaney C Pagliuso Devavrat M Bodas Amy E Pasquinelli
author_facet	Delaney C Pagliuso Devavrat M Bodas Amy E Pasquinelli
author_sort	Delaney C Pagliuso
title	Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.
title_short	Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.
title_full	Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.
title_fullStr	Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.
title_full_unstemmed	Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.
title_sort	recovery from heat shock requires the microrna pathway in caenorhabditis elegans.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4925b37160fc4debb8af21e5912cd790
work_keys_str_mv	AT delaneycpagliuso recoveryfromheatshockrequiresthemicrornapathwayincaenorhabditiselegans AT devavratmbodas recoveryfromheatshockrequiresthemicrornapathwayincaenorhabditiselegans AT amyepasquinelli recoveryfromheatshockrequiresthemicrornapathwayincaenorhabditiselegans
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Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.

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