Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer

The small leucine-rich proteoglycan (SLRP) family is widely expressed in extracellular matrix and aggravates tumor progression. However, epiphycan (EPYC), as a member of the SLRPs family, its biological function in cancer has not been confirmed. Thus, we aimed to clarify the role of EPYC in progress...

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Autores principales:	Lu Deng, Dandan Wang, Shouzhen Chen, Weiguo Hu, Ru Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	epiphycan SLRPs ovarian cancer metastasis invasion Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/492ab741ed204abb8071ad37dc5804dc
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spelling	oai:doaj.org-article:492ab741ed204abb8071ad37dc5804dc2021-11-30T12:46:43ZEpiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer2234-943X10.3389/fonc.2021.653782https://doaj.org/article/492ab741ed204abb8071ad37dc5804dc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.653782/fullhttps://doaj.org/toc/2234-943XThe small leucine-rich proteoglycan (SLRP) family is widely expressed in extracellular matrix and aggravates tumor progression. However, epiphycan (EPYC), as a member of the SLRPs family, its biological function in cancer has not been confirmed. Thus, we aimed to clarify the role of EPYC in progression of ovarian cancer (OC), and further analyze the molecular mechanisms implicated in tumorigenesis. Here, we analyzed the differential expression genes of GSE38734, including 4 matched primary OC and metastatic tissues. We obtained OC RNAseqs data from the Cancer Genome Atlas (TCGA) and analyzed the correlation between EPYC expression and OC staging, pathological grading, etc. The expression of EPYC in OC and normal ovarian tissues was compared in Oncomine website. We used siRNAs to interfere the expression of EPYC in ovarian cancer cell line SKOV3. Scratch test, transwell-matrigel chamber, CCK8 assay were used to detect the changes of SKOV3 migration, invasion and proliferation ability after EPYC was interfered. We used R software to make GO and KEGG analysis of related genes of EPYC. We used the Hitpredict website to predict interacting proteins. The results showed that the expression of EPYC in metastatic ovarian cancer was higher than primary ovarian cancer, and that in primary cancer was higher than normal ovaries. After siRNA interferes with EPYC expression, the migration, invasion and proliferation of SKOV3 cells were weakened. EPYC mainly played a role in ECM organization, and involved in PI3K/Akt, focal adhesion signaling pathways. EPYC might interact with PLCG2 and CRK, and be involved in signal transduction.Lu DengLu DengDandan WangShouzhen ChenWeiguo HuRu ZhangFrontiers Media S.A.articleepiphycanSLRPsovarian cancermetastasisinvasionNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	epiphycan SLRPs ovarian cancer metastasis invasion Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	epiphycan SLRPs ovarian cancer metastasis invasion Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Lu Deng Lu Deng Dandan Wang Shouzhen Chen Weiguo Hu Ru Zhang Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer
description	The small leucine-rich proteoglycan (SLRP) family is widely expressed in extracellular matrix and aggravates tumor progression. However, epiphycan (EPYC), as a member of the SLRPs family, its biological function in cancer has not been confirmed. Thus, we aimed to clarify the role of EPYC in progression of ovarian cancer (OC), and further analyze the molecular mechanisms implicated in tumorigenesis. Here, we analyzed the differential expression genes of GSE38734, including 4 matched primary OC and metastatic tissues. We obtained OC RNAseqs data from the Cancer Genome Atlas (TCGA) and analyzed the correlation between EPYC expression and OC staging, pathological grading, etc. The expression of EPYC in OC and normal ovarian tissues was compared in Oncomine website. We used siRNAs to interfere the expression of EPYC in ovarian cancer cell line SKOV3. Scratch test, transwell-matrigel chamber, CCK8 assay were used to detect the changes of SKOV3 migration, invasion and proliferation ability after EPYC was interfered. We used R software to make GO and KEGG analysis of related genes of EPYC. We used the Hitpredict website to predict interacting proteins. The results showed that the expression of EPYC in metastatic ovarian cancer was higher than primary ovarian cancer, and that in primary cancer was higher than normal ovaries. After siRNA interferes with EPYC expression, the migration, invasion and proliferation of SKOV3 cells were weakened. EPYC mainly played a role in ECM organization, and involved in PI3K/Akt, focal adhesion signaling pathways. EPYC might interact with PLCG2 and CRK, and be involved in signal transduction.
format	article
author	Lu Deng Lu Deng Dandan Wang Shouzhen Chen Weiguo Hu Ru Zhang
author_facet	Lu Deng Lu Deng Dandan Wang Shouzhen Chen Weiguo Hu Ru Zhang
author_sort	Lu Deng
title	Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer
title_short	Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer
title_full	Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer
title_fullStr	Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer
title_full_unstemmed	Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer
title_sort	epiphycan predicts poor outcomes and promotes metastasis in ovarian cancer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/492ab741ed204abb8071ad37dc5804dc
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_version_	1718406558913658880

Epiphycan Predicts Poor Outcomes and Promotes Metastasis in Ovarian Cancer

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