Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent

Xingui Peng,1 Cong Li,2 Yingying Bai,1 Xinyi Wang,1 Yi Zhang,1 Yanli An,1 Gao-Jun Teng,1 Shenghong Ju1 1Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, 2Key Laboratory of Smart Drug Delivery, Min...

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Autores principales:	Peng XG, Li C, Bai Y, Wang X, Zhang Y, An Y, Teng GJ, Ju S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Endothelial progenitor cell ischemia regeneration cell tracking magnetic resonance imaging optical imaging Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4946f69258a74eb191a5950eb6b26bb2
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spelling	oai:doaj.org-article:4946f69258a74eb191a5950eb6b26bb22021-12-02T01:27:01ZNoninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent1178-2013https://doaj.org/article/4946f69258a74eb191a5950eb6b26bb22018-03-01T00:00:00Zhttps://www.dovepress.com/noninvasive-evaluation-of-the-migration-effect-of-transplanted-endothe-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xingui Peng,1 Cong Li,2 Yingying Bai,1 Xinyi Wang,1 Yi Zhang,1 Yanli An,1 Gao-Jun Teng,1 Shenghong Ju1 1Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, 2Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, School of Pharmacy, Fudan University, Shanghai, People’s Republic of China Background: Endothelial progenitor cells (EPCs) play an important role in repairing ischemia tissues. However, the survival, migration and therapeutic efficacy of EPCs after transplantation need to be better understood for further cell therapy. Purpose: This study investigated the migration effect of EPCs labeled with a multimodal imaging agent in a murine ischemic hindlimb model, using magnetic resonance imaging (MRI) and optical imaging after transplantation. Methods: EPCs derived from mouse bone marrow were labeled with a multimodal imaging agent and were administered through intracardiac delivery to mice with ischemic hindlimbs. The injected EPCs and their migration effect were observed via MRI and optical imaging in vivo, and then compared to a reference standard based on histological data. The quantification of gadolinium in tissue samples was done using inductively coupled plasma mass spectrometry (ICP-MS). Results: Using in vivo MRI and optical imaging, the labeled EPCs were observed to migrate to ischemic muscle on days 3–5 after injection, while ex vivo, the EPCs were observed in the capillary vessels of the injured tissue. There were significant linear correlations between the Gd contents measured using ICP-MS in samples from the ischemic hindlimbs and livers and T1 relaxation times calculated using MRI, as well as the average fluorescence signal intensities recorded in optical images (T1 relaxation time: r=0.491; average signal from optical imaging: r=0.704, P<0.01). EPC treatment upregulated the levels of C-X-C chemokine receptor 4 and vascular endothelial growth factor (VEGF) receptor 2 and enhanced the expression of stromal cell-derived factor-1 and VEGF. Conclusion: Transplanted EPCs can be monitored with noninvasive MRI and optical imaging in vivo and were found to enhance the paracrine secretion of angiogenic factors. Keywords: endothelial progenitor cell, ischemia, regeneration, cell tracking, magnetic resonance imaging, optical imagingPeng XGLi CBai YWang XZhang YAn YTeng GJJu SDove Medical PressarticleEndothelial progenitor cellischemiaregenerationcell trackingmagnetic resonance imagingoptical imagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1819-1829 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Endothelial progenitor cell ischemia regeneration cell tracking magnetic resonance imaging optical imaging Medicine (General) R5-920
spellingShingle	Endothelial progenitor cell ischemia regeneration cell tracking magnetic resonance imaging optical imaging Medicine (General) R5-920 Peng XG Li C Bai Y Wang X Zhang Y An Y Teng GJ Ju S Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
description	Xingui Peng,1 Cong Li,2 Yingying Bai,1 Xinyi Wang,1 Yi Zhang,1 Yanli An,1 Gao-Jun Teng,1 Shenghong Ju1 1Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, 2Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, School of Pharmacy, Fudan University, Shanghai, People’s Republic of China Background: Endothelial progenitor cells (EPCs) play an important role in repairing ischemia tissues. However, the survival, migration and therapeutic efficacy of EPCs after transplantation need to be better understood for further cell therapy. Purpose: This study investigated the migration effect of EPCs labeled with a multimodal imaging agent in a murine ischemic hindlimb model, using magnetic resonance imaging (MRI) and optical imaging after transplantation. Methods: EPCs derived from mouse bone marrow were labeled with a multimodal imaging agent and were administered through intracardiac delivery to mice with ischemic hindlimbs. The injected EPCs and their migration effect were observed via MRI and optical imaging in vivo, and then compared to a reference standard based on histological data. The quantification of gadolinium in tissue samples was done using inductively coupled plasma mass spectrometry (ICP-MS). Results: Using in vivo MRI and optical imaging, the labeled EPCs were observed to migrate to ischemic muscle on days 3–5 after injection, while ex vivo, the EPCs were observed in the capillary vessels of the injured tissue. There were significant linear correlations between the Gd contents measured using ICP-MS in samples from the ischemic hindlimbs and livers and T1 relaxation times calculated using MRI, as well as the average fluorescence signal intensities recorded in optical images (T1 relaxation time: r=0.491; average signal from optical imaging: r=0.704, P<0.01). EPC treatment upregulated the levels of C-X-C chemokine receptor 4 and vascular endothelial growth factor (VEGF) receptor 2 and enhanced the expression of stromal cell-derived factor-1 and VEGF. Conclusion: Transplanted EPCs can be monitored with noninvasive MRI and optical imaging in vivo and were found to enhance the paracrine secretion of angiogenic factors. Keywords: endothelial progenitor cell, ischemia, regeneration, cell tracking, magnetic resonance imaging, optical imaging
format	article
author	Peng XG Li C Bai Y Wang X Zhang Y An Y Teng GJ Ju S
author_facet	Peng XG Li C Bai Y Wang X Zhang Y An Y Teng GJ Ju S
author_sort	Peng XG
title	Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
title_short	Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
title_full	Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
title_fullStr	Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
title_full_unstemmed	Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
title_sort	noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/4946f69258a74eb191a5950eb6b26bb2
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Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent

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