QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides

Abstract Lens ervoides, a wild relative of lentil is an important source of allelic diversity for enhancing the genetic resistance of the cultivated species against economically important fungal diseases, such as anthracnose and Stemphylium blight caused by Colletotrichum lentis and Stemphylium botr...

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Autores principales:	Vijai Bhadauria, Larissa Ramsay, Kirstin E. Bett, Sabine Banniza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/494c8ac17e564d0ca2b55769b653c9dc
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spelling	oai:doaj.org-article:494c8ac17e564d0ca2b55769b653c9dc2021-12-02T15:05:37ZQTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides10.1038/s41598-017-03463-92045-2322https://doaj.org/article/494c8ac17e564d0ca2b55769b653c9dc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03463-9https://doaj.org/toc/2045-2322Abstract Lens ervoides, a wild relative of lentil is an important source of allelic diversity for enhancing the genetic resistance of the cultivated species against economically important fungal diseases, such as anthracnose and Stemphylium blight caused by Colletotrichum lentis and Stemphylium botryosum, respectively. To unravel the genetic control underlying resistance to these fungal diseases, a recombinant inbred line (RIL) population (n = 94, F9) originating from a cross between two L. ervoides accessions, L01-827A and IG 72815, was genotyped on the Illumina HiSeq 2500 platform. A total of 289.07 million 100 bp paired-end reads were generated, giving an average 7.53-fold genomic coverage to the RILs and identifying 2,180 high-quality SNPs that assembled in 543 unique haplotypes. Seven linkage groups were resolved among haplotypes, equal to the haploid chromosome number in L. ervoides. The genetic map spanned a cumulative distance of 740.94 cM. Composite interval mapping revealed five QTLs with a significant association with resistance to C. lentis race 0, six QTLs for C. lentis race 1 resistance, and three QTLs for S. botryosum resistance. Taken together, the data obtained in the study reveal that the expression of resistance to fungal diseases in L. ervoides is a result of rearrangement of resistant alleles contributed by both parental accessions.Vijai BhadauriaLarissa RamsayKirstin E. BettSabine BannizaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vijai Bhadauria Larissa Ramsay Kirstin E. Bett Sabine Banniza QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
description	Abstract Lens ervoides, a wild relative of lentil is an important source of allelic diversity for enhancing the genetic resistance of the cultivated species against economically important fungal diseases, such as anthracnose and Stemphylium blight caused by Colletotrichum lentis and Stemphylium botryosum, respectively. To unravel the genetic control underlying resistance to these fungal diseases, a recombinant inbred line (RIL) population (n = 94, F9) originating from a cross between two L. ervoides accessions, L01-827A and IG 72815, was genotyped on the Illumina HiSeq 2500 platform. A total of 289.07 million 100 bp paired-end reads were generated, giving an average 7.53-fold genomic coverage to the RILs and identifying 2,180 high-quality SNPs that assembled in 543 unique haplotypes. Seven linkage groups were resolved among haplotypes, equal to the haploid chromosome number in L. ervoides. The genetic map spanned a cumulative distance of 740.94 cM. Composite interval mapping revealed five QTLs with a significant association with resistance to C. lentis race 0, six QTLs for C. lentis race 1 resistance, and three QTLs for S. botryosum resistance. Taken together, the data obtained in the study reveal that the expression of resistance to fungal diseases in L. ervoides is a result of rearrangement of resistant alleles contributed by both parental accessions.
format	article
author	Vijai Bhadauria Larissa Ramsay Kirstin E. Bett Sabine Banniza
author_facet	Vijai Bhadauria Larissa Ramsay Kirstin E. Bett Sabine Banniza
author_sort	Vijai Bhadauria
title	QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
title_short	QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
title_full	QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
title_fullStr	QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
title_full_unstemmed	QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
title_sort	qtl mapping reveals genetic determinants of fungal disease resistance in the wild lentil species lens ervoides
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/494c8ac17e564d0ca2b55769b653c9dc
work_keys_str_mv	AT vijaibhadauria qtlmappingrevealsgeneticdeterminantsoffungaldiseaseresistanceinthewildlentilspecieslenservoides AT larissaramsay qtlmappingrevealsgeneticdeterminantsoffungaldiseaseresistanceinthewildlentilspecieslenservoides AT kirstinebett qtlmappingrevealsgeneticdeterminantsoffungaldiseaseresistanceinthewildlentilspecieslenservoides AT sabinebanniza qtlmappingrevealsgeneticdeterminantsoffungaldiseaseresistanceinthewildlentilspecieslenservoides
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QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides

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