The effect of ZnO nanoparticles on liver function in rats

Hua-Qiao Tang, Min Xu, Qian Rong, Ru-Wen Jin, Qi-Ji Liu, Ying-Lun Li Department of Pharmacy, Sichuan Agricultural University, Sichuan, Chengdu, People’s Republic of China Abstract: Zinc oxide (ZnO) is widely incorporated as a food additive in animal diets. In order to optimize the benefi...

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Autores principales:	Tang HQ, Xu M, Rong Q, Jin RW, Liu QJ, Li YL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	ZnO nanoparticles liver injury CYP 450 enzymes Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/495cdb8ecafa40738ab138955adad11f
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spelling	oai:doaj.org-article:495cdb8ecafa40738ab138955adad11f2021-12-02T00:45:57ZThe effect of ZnO nanoparticles on liver function in rats1178-2013https://doaj.org/article/495cdb8ecafa40738ab138955adad11f2016-08-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-zno-nanoparticles-on-liver-function-in-rats-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hua-Qiao Tang, Min Xu, Qian Rong, Ru-Wen Jin, Qi-Ji Liu, Ying-Lun Li Department of Pharmacy, Sichuan Agricultural University, Sichuan, Chengdu, People’s Republic of China Abstract: Zinc oxide (ZnO) is widely incorporated as a food additive in animal diets. In order to optimize the beneficial effects of ZnO and minimize any resultant environmental pollution, ZnO nanoparticles are often used for delivery of the zinc. However, the possible toxic effects of ZnO nanoparticles, including effects on cytochrome P450 (CYP450) enzymes, have not been evaluated. In this study, we investigated the effect of ZnO nanoparticles, in doses used in animal feeds, on CYP450 enzymes, liver and intestinal enzymes, liver and kidney histopathology, and hematologic indices in rats. We found that liver and kidney injury occurred when the concentrations of ZnO nanoparticles in feed were 300–600 mg/kg. Also, liver mRNA expression for constitutive androstane receptor was suppressed and mRNA expression for pregnane X receptor was induced when feed containing ZnO nanoparticles was given at a concentration of 600 mg/kg. Although the expression of mRNA for CYP 2C11 and 3A2 enzymes was induced by ZnO nanoparticles, the activities of CYP 2C11 and 3A2 were suppressed. While liver CYP 1A2 mRNA expression was suppressed, CYP 1A2 activity remained unchanged at all ZnO nanoparticle doses. Therefore, it has been concluded that ZnO nanoparticles, in the doses customarily added to animal feed, changed the indices of hematology and blood chemistry, altered the expression and activity of hepatic CYP enzymes, and induced pathological changes in liver and kidney tissues of rats. These findings suggest that greater attention needs to be paid to the toxic effects of ZnO nanoparticles in animal feed, with the possibility that the doses of ZnO should be reduced. Keywords: ZnO nanoparticles, liver injury, CYP450 enzymesTang HQXu MRong QJin RWLiu QJLi YLDove Medical PressarticleZnO nanoparticlesliver injuryCYP 450 enzymesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4275-4285 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	ZnO nanoparticles liver injury CYP 450 enzymes Medicine (General) R5-920
spellingShingle	ZnO nanoparticles liver injury CYP 450 enzymes Medicine (General) R5-920 Tang HQ Xu M Rong Q Jin RW Liu QJ Li YL The effect of ZnO nanoparticles on liver function in rats
description	Hua-Qiao Tang, Min Xu, Qian Rong, Ru-Wen Jin, Qi-Ji Liu, Ying-Lun Li Department of Pharmacy, Sichuan Agricultural University, Sichuan, Chengdu, People’s Republic of China Abstract: Zinc oxide (ZnO) is widely incorporated as a food additive in animal diets. In order to optimize the beneficial effects of ZnO and minimize any resultant environmental pollution, ZnO nanoparticles are often used for delivery of the zinc. However, the possible toxic effects of ZnO nanoparticles, including effects on cytochrome P450 (CYP450) enzymes, have not been evaluated. In this study, we investigated the effect of ZnO nanoparticles, in doses used in animal feeds, on CYP450 enzymes, liver and intestinal enzymes, liver and kidney histopathology, and hematologic indices in rats. We found that liver and kidney injury occurred when the concentrations of ZnO nanoparticles in feed were 300–600 mg/kg. Also, liver mRNA expression for constitutive androstane receptor was suppressed and mRNA expression for pregnane X receptor was induced when feed containing ZnO nanoparticles was given at a concentration of 600 mg/kg. Although the expression of mRNA for CYP 2C11 and 3A2 enzymes was induced by ZnO nanoparticles, the activities of CYP 2C11 and 3A2 were suppressed. While liver CYP 1A2 mRNA expression was suppressed, CYP 1A2 activity remained unchanged at all ZnO nanoparticle doses. Therefore, it has been concluded that ZnO nanoparticles, in the doses customarily added to animal feed, changed the indices of hematology and blood chemistry, altered the expression and activity of hepatic CYP enzymes, and induced pathological changes in liver and kidney tissues of rats. These findings suggest that greater attention needs to be paid to the toxic effects of ZnO nanoparticles in animal feed, with the possibility that the doses of ZnO should be reduced. Keywords: ZnO nanoparticles, liver injury, CYP450 enzymes
format	article
author	Tang HQ Xu M Rong Q Jin RW Liu QJ Li YL
author_facet	Tang HQ Xu M Rong Q Jin RW Liu QJ Li YL
author_sort	Tang HQ
title	The effect of ZnO nanoparticles on liver function in rats
title_short	The effect of ZnO nanoparticles on liver function in rats
title_full	The effect of ZnO nanoparticles on liver function in rats
title_fullStr	The effect of ZnO nanoparticles on liver function in rats
title_full_unstemmed	The effect of ZnO nanoparticles on liver function in rats
title_sort	effect of zno nanoparticles on liver function in rats
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/495cdb8ecafa40738ab138955adad11f
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The effect of ZnO nanoparticles on liver function in rats

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