Antibiotic resistance genes in layer farms and their correlation with environmental samples
ABSTRACT: Livestock farms are generally considered to be the important source of antibiotic resistance genes (ARGs). It is important to explore the spread of ARGs to reduce their harm. This study analyzed 13 resistance genes belonging to 7 types in 68 samples of layer manure including different stag...
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2021
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oai:doaj.org-article:496023adfb3446149c20a7af371fd11b2021-11-24T04:22:16ZAntibiotic resistance genes in layer farms and their correlation with environmental samples0032-579110.1016/j.psj.2021.101485https://doaj.org/article/496023adfb3446149c20a7af371fd11b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005071https://doaj.org/toc/0032-5791ABSTRACT: Livestock farms are generally considered to be the important source of antibiotic resistance genes (ARGs). It is important to explore the spread of ARGs to reduce their harm. This study analyzed 13 resistance genes belonging to 7 types in 68 samples of layer manure including different stages of layer breeding, layer manure fertilizer, and soil from 9 laying hen farms in Guangdong Province. The detection rate of antibiotic resistance genes was extremely high at the layer farm in manure (100%), layer manure fertilizer (100%), and soil (> 95%). The log counts of antibiotic resistance genes in layer manure (3.34–11.83 log copies/g) were significantly higher than those in layer manure fertilizer (3.45–9.80 log copies/g) and soil (0–7.69 log copies/g). In layer manure, ermB was the most abundant antibiotic resistance gene, with a concentration of 3.19 × 109– 6.82 × 1011 copies/g. The average abundances of 5 antibiotic resistance genes were above 1010 copies/g in the descending order ermB, sul2, tetA, sul1, and strB. The relative abundances of ARGs in layer manure samples from different breeding stages ranked as follows: brooding period (BP), late laying period (LL), growing period (GP), early laying period (EL), and peak laying period (PL). There was no significant correlation between the farm scale and the abundance of antibiotic resistance genes. Moreover, the farther away from the layer farm, the lower the abundance of antibiotic resistance genes in the soil. We also found that compost increases the correlation between antibiotic resistance genes, and the antibiotic resistance genes in soil may be directly derived from layer manure fertilizer instead of manure. Therefore, when applying layer manure fertilizer to cultivated land, the risk of antibiotic resistance genes pollution should be acknowledged, and in-depth research should be conducted on how to remove antibiotic resistance genes from layer manure fertilizer to control the spread of antibiotic resistance genes.Ting ZhuTao ChenZhen CaoShan ZhongXin WenJiandui MiBaohua MaYongde ZouNa ZhangXindi LiaoYan WangYinbao WuElsevierarticlelayer manuresoilcompostantibiotic resistance geneAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101485- (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
layer manure soil compost antibiotic resistance gene Animal culture SF1-1100 |
| spellingShingle |
layer manure soil compost antibiotic resistance gene Animal culture SF1-1100 Ting Zhu Tao Chen Zhen Cao Shan Zhong Xin Wen Jiandui Mi Baohua Ma Yongde Zou Na Zhang Xindi Liao Yan Wang Yinbao Wu Antibiotic resistance genes in layer farms and their correlation with environmental samples |
| description |
ABSTRACT: Livestock farms are generally considered to be the important source of antibiotic resistance genes (ARGs). It is important to explore the spread of ARGs to reduce their harm. This study analyzed 13 resistance genes belonging to 7 types in 68 samples of layer manure including different stages of layer breeding, layer manure fertilizer, and soil from 9 laying hen farms in Guangdong Province. The detection rate of antibiotic resistance genes was extremely high at the layer farm in manure (100%), layer manure fertilizer (100%), and soil (> 95%). The log counts of antibiotic resistance genes in layer manure (3.34–11.83 log copies/g) were significantly higher than those in layer manure fertilizer (3.45–9.80 log copies/g) and soil (0–7.69 log copies/g). In layer manure, ermB was the most abundant antibiotic resistance gene, with a concentration of 3.19 × 109– 6.82 × 1011 copies/g. The average abundances of 5 antibiotic resistance genes were above 1010 copies/g in the descending order ermB, sul2, tetA, sul1, and strB. The relative abundances of ARGs in layer manure samples from different breeding stages ranked as follows: brooding period (BP), late laying period (LL), growing period (GP), early laying period (EL), and peak laying period (PL). There was no significant correlation between the farm scale and the abundance of antibiotic resistance genes. Moreover, the farther away from the layer farm, the lower the abundance of antibiotic resistance genes in the soil. We also found that compost increases the correlation between antibiotic resistance genes, and the antibiotic resistance genes in soil may be directly derived from layer manure fertilizer instead of manure. Therefore, when applying layer manure fertilizer to cultivated land, the risk of antibiotic resistance genes pollution should be acknowledged, and in-depth research should be conducted on how to remove antibiotic resistance genes from layer manure fertilizer to control the spread of antibiotic resistance genes. |
| format |
article |
| author |
Ting Zhu Tao Chen Zhen Cao Shan Zhong Xin Wen Jiandui Mi Baohua Ma Yongde Zou Na Zhang Xindi Liao Yan Wang Yinbao Wu |
| author_facet |
Ting Zhu Tao Chen Zhen Cao Shan Zhong Xin Wen Jiandui Mi Baohua Ma Yongde Zou Na Zhang Xindi Liao Yan Wang Yinbao Wu |
| author_sort |
Ting Zhu |
| title |
Antibiotic resistance genes in layer farms and their correlation with environmental samples |
| title_short |
Antibiotic resistance genes in layer farms and their correlation with environmental samples |
| title_full |
Antibiotic resistance genes in layer farms and their correlation with environmental samples |
| title_fullStr |
Antibiotic resistance genes in layer farms and their correlation with environmental samples |
| title_full_unstemmed |
Antibiotic resistance genes in layer farms and their correlation with environmental samples |
| title_sort |
antibiotic resistance genes in layer farms and their correlation with environmental samples |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/496023adfb3446149c20a7af371fd11b |
| work_keys_str_mv |
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