Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium

ABSTRACT Since its emergence in 2019, circulating populations of the new coronavirus (CoV) continuously acquired genetic diversity. At the end of 2020, a variant named 20I/501Y.V1 (lineage B.1.1.7) emerged and replaced other circulating strains in several regions. This phenomenon has been poorly ass...

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Autores principales:	Franck Touret, Léa Luciani, Cécile Baronti, Maxime Cochin, Jean-Sélim Driouich, Magali Gilles, Laurence Thirion, Antoine Nougairède, Xavier de Lamballerie
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	20I/501Y.V1 B.1.1.7 SARS-CoV-2 ex vivo in vitro replicative fitness Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/49609bfe41d84d1ebf5651a0b0d86f6a
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spelling	oai:doaj.org-article:49609bfe41d84d1ebf5651a0b0d86f6a2021-11-10T18:37:52ZReplicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium10.1128/mBio.00850-212150-7511https://doaj.org/article/49609bfe41d84d1ebf5651a0b0d86f6a2021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00850-21https://doaj.org/toc/2150-7511ABSTRACT Since its emergence in 2019, circulating populations of the new coronavirus (CoV) continuously acquired genetic diversity. At the end of 2020, a variant named 20I/501Y.V1 (lineage B.1.1.7) emerged and replaced other circulating strains in several regions. This phenomenon has been poorly associated with biological evidence that this variant and the original strain exhibit different phenotypic characteristics. Here, we analyze the replication ability of this new variant in different cellular models using for comparison an ancestral D614G European strain (lineage B1). Results from comparative replication kinetics experiments in vitro and in a human reconstituted bronchial epithelium showed no difference. However, when both viruses were put in competition in human reconstituted bronchial epithelium, the 20I/501Y.V1 variant outcompeted the ancestral strain. All together, these findings demonstrate that this new variant replicates more efficiently and may contribute to a better understanding of the progressive replacement of circulating strains by the severe acute respiratory CoV-2 (SARS-CoV-2) 20I/501Y.V1 variant. IMPORTANCE The emergence of several SARS-CoV-2 variants raised numerous questions concerning the future course of the pandemic. We are currently observing a replacement of the circulating viruses by the variant from the United Kingdom known as 20I/501Y.V1, from the B.1.1.7 lineage, but there is little biological evidence that this new variant exhibits a different phenotype. In the present study, we used different cellular models to assess the replication ability of the 20I/501Y.V1 variant. Our results showed that this variant replicates more efficiently in human reconstituted bronchial epithelium, which may explain why it spreads so rapidly in human populations.Franck TouretLéa LucianiCécile BarontiMaxime CochinJean-Sélim DriouichMagali GillesLaurence ThirionAntoine NougairèdeXavier de LamballerieAmerican Society for Microbiologyarticle20I/501Y.V1B.1.1.7SARS-CoV-2ex vivoin vitroreplicative fitnessMicrobiologyQR1-502ENmBio, Vol 12, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	20I/501Y.V1 B.1.1.7 SARS-CoV-2 ex vivo in vitro replicative fitness Microbiology QR1-502
spellingShingle	20I/501Y.V1 B.1.1.7 SARS-CoV-2 ex vivo in vitro replicative fitness Microbiology QR1-502 Franck Touret Léa Luciani Cécile Baronti Maxime Cochin Jean-Sélim Driouich Magali Gilles Laurence Thirion Antoine Nougairède Xavier de Lamballerie Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium
description	ABSTRACT Since its emergence in 2019, circulating populations of the new coronavirus (CoV) continuously acquired genetic diversity. At the end of 2020, a variant named 20I/501Y.V1 (lineage B.1.1.7) emerged and replaced other circulating strains in several regions. This phenomenon has been poorly associated with biological evidence that this variant and the original strain exhibit different phenotypic characteristics. Here, we analyze the replication ability of this new variant in different cellular models using for comparison an ancestral D614G European strain (lineage B1). Results from comparative replication kinetics experiments in vitro and in a human reconstituted bronchial epithelium showed no difference. However, when both viruses were put in competition in human reconstituted bronchial epithelium, the 20I/501Y.V1 variant outcompeted the ancestral strain. All together, these findings demonstrate that this new variant replicates more efficiently and may contribute to a better understanding of the progressive replacement of circulating strains by the severe acute respiratory CoV-2 (SARS-CoV-2) 20I/501Y.V1 variant. IMPORTANCE The emergence of several SARS-CoV-2 variants raised numerous questions concerning the future course of the pandemic. We are currently observing a replacement of the circulating viruses by the variant from the United Kingdom known as 20I/501Y.V1, from the B.1.1.7 lineage, but there is little biological evidence that this new variant exhibits a different phenotype. In the present study, we used different cellular models to assess the replication ability of the 20I/501Y.V1 variant. Our results showed that this variant replicates more efficiently in human reconstituted bronchial epithelium, which may explain why it spreads so rapidly in human populations.
format	article
author	Franck Touret Léa Luciani Cécile Baronti Maxime Cochin Jean-Sélim Driouich Magali Gilles Laurence Thirion Antoine Nougairède Xavier de Lamballerie
author_facet	Franck Touret Léa Luciani Cécile Baronti Maxime Cochin Jean-Sélim Driouich Magali Gilles Laurence Thirion Antoine Nougairède Xavier de Lamballerie
author_sort	Franck Touret
title	Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium
title_short	Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium
title_full	Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium
title_fullStr	Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium
title_full_unstemmed	Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium
title_sort	replicative fitness of a sars-cov-2 20i/501y.v1 variant from lineage b.1.1.7 in human reconstituted bronchial epithelium
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/49609bfe41d84d1ebf5651a0b0d86f6a
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Replicative Fitness of a SARS-CoV-2 20I/501Y.V1 Variant from Lineage B.1.1.7 in Human Reconstituted Bronchial Epithelium

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