Optimization of lipase production using fungal isolates from oily residues

Abstract Lipases are triacylglycerol hydrolases that catalyze hydrolysis, esterification, interesterification, and transesterification reactions. These enzymes are targets of several industrial and biotech applications, such as catalysts, detergent production, food, biofuels, wastewater treatment, a...

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Autores principales:	Leticia Miranda Cesário, Giovanna Pinto Pires, Rafael Freitas Santos Pereira, Elisabete Fantuzzi, André da Silva Xavier, Servio Tulio Alves Cassini, Jairo Pinto de Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Fungal lipases Optimization Factorial design Oily waste Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/4960dbacabdf4e3db8b046182e0cb81f
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spelling	oai:doaj.org-article:4960dbacabdf4e3db8b046182e0cb81f2021-11-14T12:31:51ZOptimization of lipase production using fungal isolates from oily residues10.1186/s12896-021-00724-41472-6750https://doaj.org/article/4960dbacabdf4e3db8b046182e0cb81f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12896-021-00724-4https://doaj.org/toc/1472-6750Abstract Lipases are triacylglycerol hydrolases that catalyze hydrolysis, esterification, interesterification, and transesterification reactions. These enzymes are targets of several industrial and biotech applications, such as catalysts, detergent production, food, biofuels, wastewater treatment, and others. Microbial enzymes are preferable for large scale production due to ease of production and extraction. Several studies have reported that lipases from filamentous fungi are predominantly extracellular and highly active. However, there are many factors that interfere with enzyme production (pH, temperature, medium composition, agitation, aeration, inducer type, and concentration, etc.), making control difficult and burdening the process. This work aimed to optimize the lipase production of four fungal isolates from oily residues (Penicillium sp., Aspergillus niger, Aspergillus sp., and Aspergillus sp.). The lipase-producing fungi isolates were morphologically characterized by optical and scanning electron microscopy. The optimal lipase production time curve was previously determined, and the response variable used was the amount of total protein in the medium after cultivation by submerged fermentation. A complete factorial design 32 was performed, evaluating the temperatures (28 °C, 32 °C, and 36 °C) and soybean oil inducer concentration (2%, 6%, and 10%). Each lipase-producing isolate reacted differently to the conditions tested, the Aspergillus sp. F18 reached maximum lipase production, compared to others, under conditions of 32 °C and 2% of oil with a yield of 11,007 (µg mL−1). Penicillium sp. F04 achieved better results at 36 °C and 6% oil, although for Aspergillus niger F16 was at 36 °C and 10% oil and Aspergillus sp. F21 at 32 °C and 2% oil. These results show that microorganisms isolated from oily residues derived from environmental sanitation can be a promising alternative for the large-scale production of lipases. Graphical AbstractLeticia Miranda CesárioGiovanna Pinto PiresRafael Freitas Santos PereiraElisabete FantuzziAndré da Silva XavierServio Tulio Alves CassiniJairo Pinto de OliveiraBMCarticleFungal lipasesOptimizationFactorial designOily wasteBiotechnologyTP248.13-248.65ENBMC Biotechnology, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fungal lipases Optimization Factorial design Oily waste Biotechnology TP248.13-248.65
spellingShingle	Fungal lipases Optimization Factorial design Oily waste Biotechnology TP248.13-248.65 Leticia Miranda Cesário Giovanna Pinto Pires Rafael Freitas Santos Pereira Elisabete Fantuzzi André da Silva Xavier Servio Tulio Alves Cassini Jairo Pinto de Oliveira Optimization of lipase production using fungal isolates from oily residues
description	Abstract Lipases are triacylglycerol hydrolases that catalyze hydrolysis, esterification, interesterification, and transesterification reactions. These enzymes are targets of several industrial and biotech applications, such as catalysts, detergent production, food, biofuels, wastewater treatment, and others. Microbial enzymes are preferable for large scale production due to ease of production and extraction. Several studies have reported that lipases from filamentous fungi are predominantly extracellular and highly active. However, there are many factors that interfere with enzyme production (pH, temperature, medium composition, agitation, aeration, inducer type, and concentration, etc.), making control difficult and burdening the process. This work aimed to optimize the lipase production of four fungal isolates from oily residues (Penicillium sp., Aspergillus niger, Aspergillus sp., and Aspergillus sp.). The lipase-producing fungi isolates were morphologically characterized by optical and scanning electron microscopy. The optimal lipase production time curve was previously determined, and the response variable used was the amount of total protein in the medium after cultivation by submerged fermentation. A complete factorial design 32 was performed, evaluating the temperatures (28 °C, 32 °C, and 36 °C) and soybean oil inducer concentration (2%, 6%, and 10%). Each lipase-producing isolate reacted differently to the conditions tested, the Aspergillus sp. F18 reached maximum lipase production, compared to others, under conditions of 32 °C and 2% of oil with a yield of 11,007 (µg mL−1). Penicillium sp. F04 achieved better results at 36 °C and 6% oil, although for Aspergillus niger F16 was at 36 °C and 10% oil and Aspergillus sp. F21 at 32 °C and 2% oil. These results show that microorganisms isolated from oily residues derived from environmental sanitation can be a promising alternative for the large-scale production of lipases. Graphical Abstract
format	article
author	Leticia Miranda Cesário Giovanna Pinto Pires Rafael Freitas Santos Pereira Elisabete Fantuzzi André da Silva Xavier Servio Tulio Alves Cassini Jairo Pinto de Oliveira
author_facet	Leticia Miranda Cesário Giovanna Pinto Pires Rafael Freitas Santos Pereira Elisabete Fantuzzi André da Silva Xavier Servio Tulio Alves Cassini Jairo Pinto de Oliveira
author_sort	Leticia Miranda Cesário
title	Optimization of lipase production using fungal isolates from oily residues
title_short	Optimization of lipase production using fungal isolates from oily residues
title_full	Optimization of lipase production using fungal isolates from oily residues
title_fullStr	Optimization of lipase production using fungal isolates from oily residues
title_full_unstemmed	Optimization of lipase production using fungal isolates from oily residues
title_sort	optimization of lipase production using fungal isolates from oily residues
publisher	BMC
publishDate	2021
url	https://doaj.org/article/4960dbacabdf4e3db8b046182e0cb81f
work_keys_str_mv	AT leticiamirandacesario optimizationoflipaseproductionusingfungalisolatesfromoilyresidues AT giovannapintopires optimizationoflipaseproductionusingfungalisolatesfromoilyresidues AT rafaelfreitassantospereira optimizationoflipaseproductionusingfungalisolatesfromoilyresidues AT elisabetefantuzzi optimizationoflipaseproductionusingfungalisolatesfromoilyresidues AT andredasilvaxavier optimizationoflipaseproductionusingfungalisolatesfromoilyresidues AT serviotulioalvescassini optimizationoflipaseproductionusingfungalisolatesfromoilyresidues AT jairopintodeoliveira optimizationoflipaseproductionusingfungalisolatesfromoilyresidues
_version_	1718429164097241088

Optimization of lipase production using fungal isolates from oily residues

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