Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.

Pathogenic spirochetes are bacteria that cause a number of emerging and re-emerging diseases worldwide, including syphilis, leptospirosis, relapsing fever, and Lyme borreliosis. They navigate efficiently through dense extracellular matrix and cross the blood-brain barrier by unknown mechanisms. Due...

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Autores principales:	Tara J Moriarty, M Ursula Norman, Pina Colarusso, Troy Bankhead, Paul Kubes, George Chaconas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4964ec69776245e59909e226ad196f9f
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spelling	oai:doaj.org-article:4964ec69776245e59909e226ad196f9f2021-12-02T19:59:49ZReal-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.1553-73661553-737410.1371/journal.ppat.1000090https://doaj.org/article/4964ec69776245e59909e226ad196f9f2008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18566656/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Pathogenic spirochetes are bacteria that cause a number of emerging and re-emerging diseases worldwide, including syphilis, leptospirosis, relapsing fever, and Lyme borreliosis. They navigate efficiently through dense extracellular matrix and cross the blood-brain barrier by unknown mechanisms. Due to their slender morphology, spirochetes are difficult to visualize by standard light microscopy, impeding studies of their behavior in situ. We engineered a fluorescent infectious strain of Borrelia burgdorferi, the Lyme disease pathogen, which expressed green fluorescent protein (GFP). Real-time 3D and 4D quantitative analysis of fluorescent spirochete dissemination from the microvasculature of living mice at high resolution revealed that dissemination was a multi-stage process that included transient tethering-type associations, short-term dragging interactions, and stationary adhesion. Stationary adhesions and extravasating spirochetes were most commonly observed at endothelial junctions, and translational motility of spirochetes appeared to play an integral role in transendothelial migration. To our knowledge, this is the first report of high resolution 3D and 4D visualization of dissemination of a bacterial pathogen in a living mammalian host, and provides the first direct insight into spirochete dissemination in vivo.Tara J MoriartyM Ursula NormanPina ColarussoTroy BankheadPaul KubesGeorge ChaconasPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 6, p e1000090 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Tara J Moriarty M Ursula Norman Pina Colarusso Troy Bankhead Paul Kubes George Chaconas Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
description	Pathogenic spirochetes are bacteria that cause a number of emerging and re-emerging diseases worldwide, including syphilis, leptospirosis, relapsing fever, and Lyme borreliosis. They navigate efficiently through dense extracellular matrix and cross the blood-brain barrier by unknown mechanisms. Due to their slender morphology, spirochetes are difficult to visualize by standard light microscopy, impeding studies of their behavior in situ. We engineered a fluorescent infectious strain of Borrelia burgdorferi, the Lyme disease pathogen, which expressed green fluorescent protein (GFP). Real-time 3D and 4D quantitative analysis of fluorescent spirochete dissemination from the microvasculature of living mice at high resolution revealed that dissemination was a multi-stage process that included transient tethering-type associations, short-term dragging interactions, and stationary adhesion. Stationary adhesions and extravasating spirochetes were most commonly observed at endothelial junctions, and translational motility of spirochetes appeared to play an integral role in transendothelial migration. To our knowledge, this is the first report of high resolution 3D and 4D visualization of dissemination of a bacterial pathogen in a living mammalian host, and provides the first direct insight into spirochete dissemination in vivo.
format	article
author	Tara J Moriarty M Ursula Norman Pina Colarusso Troy Bankhead Paul Kubes George Chaconas
author_facet	Tara J Moriarty M Ursula Norman Pina Colarusso Troy Bankhead Paul Kubes George Chaconas
author_sort	Tara J Moriarty
title	Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
title_short	Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
title_full	Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
title_fullStr	Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
title_full_unstemmed	Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
title_sort	real-time high resolution 3d imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/4964ec69776245e59909e226ad196f9f
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Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.

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