DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA

The article presents a study on the distribution of gene polymorphisms in the histocompatibility antigens among the patients diagnosed with AML, and healthy donors in the Republic of Kazakhstan, as well as features of the HLA-A*, *B, Cw*, DRB1*, DQB1* distribution among the patients with acute myelo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	I. R. Ramilyeva, Zh. K. Burkitbaev, S. A. Abdrakhmanova, A. A. Turganbekova, D. K. Baimukasheva, E. B. Zhiburt
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	acute myeloblastic leukemia antigens hla-system molecular genetic method Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/49689018feb7499493663b7ea7dba24e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:49689018feb7499493663b7ea7dba24e
record_format	dspace
spelling	oai:doaj.org-article:49689018feb7499493663b7ea7dba24e2021-11-18T08:03:48ZDISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA1563-06252313-741X10.15789/1563-0625-2019-5-965-972https://doaj.org/article/49689018feb7499493663b7ea7dba24e2019-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1613https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe article presents a study on the distribution of gene polymorphisms in the histocompatibility antigens among the patients diagnosed with AML, and healthy donors in the Republic of Kazakhstan, as well as features of the HLA-A, B, Cw, DRB1, DQB1* distribution among the patients with acute myeloid leukemia (AML). HLA typing and data processing were performed at the Research and Production Center of Transfusiology, Nur-Sultan. A total of 3808 people were examined, including 3621 healthy blood donors and 187 patients diagnosed with AML. Genomic DNA for HLA typing was isolated from peripheral blood leukocytes by proteinase method using columns with silica membrane and using a set of reagents PROTRANS DNA BOX (Protrans, Germany). Typing of HLA-A, B, C, DRB1, DQB1 in the patients and blood donors was performed by polymerase chain reaction using commercial reagent kits from Protrans (PROTRANS HLA- A/B/DRB1* Cyclerplate System, PROTRANS HLA-C* Cyclerplate System, PROTRANS HLA-DQB1* Cyclerplate System).HLA-A31 (OR = 1.8; CI 1.16-2.79; p < 0.01) proved to be more common in the group of patients compared to the control group, which suggesting an association between AML and presence of this antigen. The control group showed an increased frequency of HLA-A02 antigen (OR = 0.55; CI 0.41-0.75; p < 0.01). This antigen may be, therefore, exert a protective effect in AML development.The studies of major histocompatibility complex which include HLA genes, did significantly expanded the understanding of HLA antigens which may have strong associative links with distinct diseases, and moderately or poorly expressed links in other disorders. Analysis of the literature data showed that myeloid leukemia is characterized by decreased frequency of HLA-B13, B14, B40 antigens, most often determined by antigens B16, Bw 22, B27. In this study, HLA-A31, B37 were associated with AML. Phenotypes with antigens HLA-A02, B27, C02, DRB101, 04, DQB106 have a probable protective effect on the development of this pathology.The study has determined some features of histocompatibility gene distribution in AML patients, detection of HLA-markers that determine the risk or resistance to the occurrence of this disease. We have established characteristic specific markers of HLA system among AML patients in Kazakhstan, which may be associated with higher risk of the disease.I. R. RamilyevaZh. K. BurkitbaevS. A. AbdrakhmanovaA. A. TurganbekovaD. K. BaimukashevaE. B. ZhiburtSPb RAACIarticleacute myeloblastic leukemiaantigenshla-systemmolecular genetic methodImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 5, Pp 965-972 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	acute myeloblastic leukemia antigens hla-system molecular genetic method Immunologic diseases. Allergy RC581-607
spellingShingle	acute myeloblastic leukemia antigens hla-system molecular genetic method Immunologic diseases. Allergy RC581-607 I. R. Ramilyeva Zh. K. Burkitbaev S. A. Abdrakhmanova A. A. Turganbekova D. K. Baimukasheva E. B. Zhiburt DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA
description	The article presents a study on the distribution of gene polymorphisms in the histocompatibility antigens among the patients diagnosed with AML, and healthy donors in the Republic of Kazakhstan, as well as features of the HLA-A, B, Cw, DRB1, DQB1* distribution among the patients with acute myeloid leukemia (AML). HLA typing and data processing were performed at the Research and Production Center of Transfusiology, Nur-Sultan. A total of 3808 people were examined, including 3621 healthy blood donors and 187 patients diagnosed with AML. Genomic DNA for HLA typing was isolated from peripheral blood leukocytes by proteinase method using columns with silica membrane and using a set of reagents PROTRANS DNA BOX (Protrans, Germany). Typing of HLA-A, B, C, DRB1, DQB1 in the patients and blood donors was performed by polymerase chain reaction using commercial reagent kits from Protrans (PROTRANS HLA- A/B/DRB1* Cyclerplate System, PROTRANS HLA-C* Cyclerplate System, PROTRANS HLA-DQB1* Cyclerplate System).HLA-A31 (OR = 1.8; CI 1.16-2.79; p < 0.01) proved to be more common in the group of patients compared to the control group, which suggesting an association between AML and presence of this antigen. The control group showed an increased frequency of HLA-A02 antigen (OR = 0.55; CI 0.41-0.75; p < 0.01). This antigen may be, therefore, exert a protective effect in AML development.The studies of major histocompatibility complex which include HLA genes, did significantly expanded the understanding of HLA antigens which may have strong associative links with distinct diseases, and moderately or poorly expressed links in other disorders. Analysis of the literature data showed that myeloid leukemia is characterized by decreased frequency of HLA-B13, B14, B40 antigens, most often determined by antigens B16, Bw 22, B27. In this study, HLA-A31, B37 were associated with AML. Phenotypes with antigens HLA-A02, B27, C02, DRB101, 04, DQB106 have a probable protective effect on the development of this pathology.The study has determined some features of histocompatibility gene distribution in AML patients, detection of HLA-markers that determine the risk or resistance to the occurrence of this disease. We have established characteristic specific markers of HLA system among AML patients in Kazakhstan, which may be associated with higher risk of the disease.
format	article
author	I. R. Ramilyeva Zh. K. Burkitbaev S. A. Abdrakhmanova A. A. Turganbekova D. K. Baimukasheva E. B. Zhiburt
author_facet	I. R. Ramilyeva Zh. K. Burkitbaev S. A. Abdrakhmanova A. A. Turganbekova D. K. Baimukasheva E. B. Zhiburt
author_sort	I. R. Ramilyeva
title	DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA
title_short	DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA
title_full	DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA
title_fullStr	DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA
title_full_unstemmed	DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA
title_sort	distribution pattern for hla specificities in the patients with acute myeloid leukemia
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/49689018feb7499493663b7ea7dba24e
work_keys_str_mv	AT irramilyeva distributionpatternforhlaspecificitiesinthepatientswithacutemyeloidleukemia AT zhkburkitbaev distributionpatternforhlaspecificitiesinthepatientswithacutemyeloidleukemia AT saabdrakhmanova distributionpatternforhlaspecificitiesinthepatientswithacutemyeloidleukemia AT aaturganbekova distributionpatternforhlaspecificitiesinthepatientswithacutemyeloidleukemia AT dkbaimukasheva distributionpatternforhlaspecificitiesinthepatientswithacutemyeloidleukemia AT ebzhiburt distributionpatternforhlaspecificitiesinthepatientswithacutemyeloidleukemia
_version_	1718422345755918336

DISTRIBUTION PATTERN FOR HLA SPECIFICITIES IN THE PATIENTS WITH ACUTE MYELOID LEUKEMIA

Ejemplares similares