PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS

The brucellosis remains to one of the most urgent dangerous infections in regions with developed livestock production. An exclusive polymorphism of symptoms, variety of forms of a disease, small informational content of results of routine laboratory all-clinical inspection, quite often leads to diag...

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Autores principales: M. V. Kostyuchenko, D. G. Ponomarenko, E. L. Rakitina, O. V. Logvinenko, I. V. Sannikova, D. A. Dejneka, O. G. Golub
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Publicado: Sankt-Peterburg : NIIÈM imeni Pastera 2017
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spelling oai:doaj.org-article:4968f47c550c49c8b7bb7ee95b0a4bbf2021-11-22T07:09:48ZPERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS2220-76192313-739810.15789/2220-7619-2017-1-91-96https://doaj.org/article/4968f47c550c49c8b7bb7ee95b0a4bbf2017-03-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/484https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The brucellosis remains to one of the most urgent dangerous infections in regions with developed livestock production. An exclusive polymorphism of symptoms, variety of forms of a disease, small informational content of results of routine laboratory all-clinical inspection, quite often leads to diagnostic mistakes at a pre-hospital stage. Improvement of a complex of laboratory diagnosis of a brucellous infection demands development of the modern padding methods of verification based on cell-like factors of immunity as leaders in an immunogenesis and a pathogenesis of a brucellosis. Considering the leading role of cell-like immunity in formation of protection against the majority of bacteriemic especially dangerous infections, studying of cell-like reaction in response to antigenic stimulation, it is necessary to consider the most informative (marker) and objective at assessment of immunologic reorganization of an organism at a disease or vaccination. The following markers (receptors) of activation of lymphocytes can act as perspective indexes of a specific cell-like antigenreactivity: CD25 — a high-affine receptor of interleukin 2 (IL-2Ra), a marker of early activation of Tlymphocytes; HLA-DR — an antigen of the main complex of a histocompatibility of a class II, an expression of a marker is associated not only with late, but also long-lived activation of lymphocytes; CD95 (Fas, APO-1) — a receptor of an induction of an apoptosis (“cell death”), a marker of “late” activation (CD4+ lymphocytes is presented mainly) and Fas L (CD178) — a receptor of an induction of an apoptosis, expresses generally on CD8+ cages. The work purpose — to estimate an opportunity and prospects of use of technology of a flowing cytofluorometry and the in vitro cell tests for diagnosis of a acute brucellosis. 35 people with the diagnosis “Acute brucellosis” and 12 people — not the patients who did not have a brucellosis, are not vaccinated against a brucellosis (control group) participated in a research. Blue blood served as material of a research. Defined quantity of the lymphocytes expressing receptors of CD25, HLA-DR, CD95, CD95L (CD178) at activation by a specific antigen. The received results processed statistically with use of Microsoft Excel 2010. During the research it is established that intensity an antigen-stimulated activation of lymphocytes of in vitro, it is possible to use as a marker of a acute brucellous infection at the person. The most perspective indexes of activation of lymphocytes of in vitro, it is possible to consider receptors to IL-2 (CD25) and apoptosis markers — CD95, CD95L (CD178). Thus, the conducted researches indicate an opportunity and the actual prospect of use of technology of a flowing cytofluorometry and cell-like in vitro tests for diagnostics of a acute brucellosis.M. V. KostyuchenkoD. G. PonomarenkoE. L. RakitinaO. V. LogvinenkoI. V. SannikovaD. A. DejnekaO. G. GolubSankt-Peterburg : NIIÈM imeni Pasteraarticlebrucellosisflow cytometryantigennegative lymphocytesdiagnostics of acute brucellosisthe test antigen stimulation of cellsmarkers of lymphocyte activationInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 1, Pp 91-96 (2017)
institution DOAJ
collection DOAJ
language RU
topic brucellosis
flow cytometry
antigennegative lymphocytes
diagnostics of acute brucellosis
the test antigen stimulation of cells
markers of lymphocyte activation
Infectious and parasitic diseases
RC109-216
spellingShingle brucellosis
flow cytometry
antigennegative lymphocytes
diagnostics of acute brucellosis
the test antigen stimulation of cells
markers of lymphocyte activation
Infectious and parasitic diseases
RC109-216
M. V. Kostyuchenko
D. G. Ponomarenko
E. L. Rakitina
O. V. Logvinenko
I. V. Sannikova
D. A. Dejneka
O. G. Golub
PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
description The brucellosis remains to one of the most urgent dangerous infections in regions with developed livestock production. An exclusive polymorphism of symptoms, variety of forms of a disease, small informational content of results of routine laboratory all-clinical inspection, quite often leads to diagnostic mistakes at a pre-hospital stage. Improvement of a complex of laboratory diagnosis of a brucellous infection demands development of the modern padding methods of verification based on cell-like factors of immunity as leaders in an immunogenesis and a pathogenesis of a brucellosis. Considering the leading role of cell-like immunity in formation of protection against the majority of bacteriemic especially dangerous infections, studying of cell-like reaction in response to antigenic stimulation, it is necessary to consider the most informative (marker) and objective at assessment of immunologic reorganization of an organism at a disease or vaccination. The following markers (receptors) of activation of lymphocytes can act as perspective indexes of a specific cell-like antigenreactivity: CD25 — a high-affine receptor of interleukin 2 (IL-2Ra), a marker of early activation of Tlymphocytes; HLA-DR — an antigen of the main complex of a histocompatibility of a class II, an expression of a marker is associated not only with late, but also long-lived activation of lymphocytes; CD95 (Fas, APO-1) — a receptor of an induction of an apoptosis (“cell death”), a marker of “late” activation (CD4+ lymphocytes is presented mainly) and Fas L (CD178) — a receptor of an induction of an apoptosis, expresses generally on CD8+ cages. The work purpose — to estimate an opportunity and prospects of use of technology of a flowing cytofluorometry and the in vitro cell tests for diagnosis of a acute brucellosis. 35 people with the diagnosis “Acute brucellosis” and 12 people — not the patients who did not have a brucellosis, are not vaccinated against a brucellosis (control group) participated in a research. Blue blood served as material of a research. Defined quantity of the lymphocytes expressing receptors of CD25, HLA-DR, CD95, CD95L (CD178) at activation by a specific antigen. The received results processed statistically with use of Microsoft Excel 2010. During the research it is established that intensity an antigen-stimulated activation of lymphocytes of in vitro, it is possible to use as a marker of a acute brucellous infection at the person. The most perspective indexes of activation of lymphocytes of in vitro, it is possible to consider receptors to IL-2 (CD25) and apoptosis markers — CD95, CD95L (CD178). Thus, the conducted researches indicate an opportunity and the actual prospect of use of technology of a flowing cytofluorometry and cell-like in vitro tests for diagnostics of a acute brucellosis.
format article
author M. V. Kostyuchenko
D. G. Ponomarenko
E. L. Rakitina
O. V. Logvinenko
I. V. Sannikova
D. A. Dejneka
O. G. Golub
author_facet M. V. Kostyuchenko
D. G. Ponomarenko
E. L. Rakitina
O. V. Logvinenko
I. V. Sannikova
D. A. Dejneka
O. G. Golub
author_sort M. V. Kostyuchenko
title PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
title_short PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
title_full PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
title_fullStr PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
title_full_unstemmed PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
title_sort perspective of in vitro lymphocytes antigenicity evaluation for the diagnostics of acute brucellosis
publisher Sankt-Peterburg : NIIÈM imeni Pastera
publishDate 2017
url https://doaj.org/article/4968f47c550c49c8b7bb7ee95b0a4bbf
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