Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae

Drug resistance is one of the problems affecting the world where drug-resistant Escherichia coli and Klebsiella pneumoniae have been shown to be ubiquitous, frequently isolated from foods and commonly associated with surgical site infection in hospitals worldwide. The aims of this work were to analy...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bashir Salim Faruk, Kumar Gaurav
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	plumbago indica drug-resistant escherichia coli klebsiella pneumoniae phytochemicals antibacterial Agriculture S Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/496b9314bc6449a794af05d53875f5e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:496b9314bc6449a794af05d53875f5e9
record_format	dspace
spelling	oai:doaj.org-article:496b9314bc6449a794af05d53875f5e92021-12-05T14:10:59ZPreliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae2391-953110.1515/opag-2021-0026https://doaj.org/article/496b9314bc6449a794af05d53875f5e92021-06-01T00:00:00Zhttps://doi.org/10.1515/opag-2021-0026https://doaj.org/toc/2391-9531Drug resistance is one of the problems affecting the world where drug-resistant Escherichia coli and Klebsiella pneumoniae have been shown to be ubiquitous, frequently isolated from foods and commonly associated with surgical site infection in hospitals worldwide. The aims of this work were to analyze the antibacterial activity of root extracts of the plant obtained by serial extraction (using petroleum ether, chloroform, methanol, and water) and by in vitro techniques and preliminary screen phytochemicals present in the extract by qualitative means. Fresh roots of Plumbago indica were collected, oven-dried, and extracted using Soxhlet apparatus; antibacterial activity, minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs) of the active extract were evaluated by standard methods against clinically isolated drug-resistant E. coli and K. pneumoniae; preliminary phytochemical screening was taken to detect the presence of alkaloids, saponins, flavonoids, steroids, tannins, reducing sugars, phenolics, protein, and oil and fat; and bioactive compounds were detected by GCMS analysis of the active extracts. Determination of antibacterial activity showed that the test organisms were susceptible to methanol and aqueous extracts only. MIC of methanolic extract was found to be 20 µg/mL on both E. coli and K. pneumoniae, while aqueous extract had MIC of 10 and 20 µg/mL on E. coli and K. pneumoniae, respectively. Preliminary phytochemical screening showed the presence of all the above-mentioned phytochemicals except oil and fat. The significance of this work is to find a lasting solution to the current problem of emerging drug-resistant bacteria (E. coli and K. pneumoniae) through the use of extracts obtained from P. indica which have long history of use as traditional medicine. The methanolic and aqueous extract can be recommended as an alternative and candidates for drug development against drug-resistant E. coli and K. pneumoniae.Bashir Salim FarukKumar GauravDe Gruyterarticleplumbago indicadrug-resistantescherichia coliklebsiella pneumoniaephytochemicalsantibacterialAgricultureSAgriculture (General)S1-972ENOpen Agriculture, Vol 6, Iss 1, Pp 435-444 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plumbago indica drug-resistant escherichia coli klebsiella pneumoniae phytochemicals antibacterial Agriculture S Agriculture (General) S1-972
spellingShingle	plumbago indica drug-resistant escherichia coli klebsiella pneumoniae phytochemicals antibacterial Agriculture S Agriculture (General) S1-972 Bashir Salim Faruk Kumar Gaurav Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae
description	Drug resistance is one of the problems affecting the world where drug-resistant Escherichia coli and Klebsiella pneumoniae have been shown to be ubiquitous, frequently isolated from foods and commonly associated with surgical site infection in hospitals worldwide. The aims of this work were to analyze the antibacterial activity of root extracts of the plant obtained by serial extraction (using petroleum ether, chloroform, methanol, and water) and by in vitro techniques and preliminary screen phytochemicals present in the extract by qualitative means. Fresh roots of Plumbago indica were collected, oven-dried, and extracted using Soxhlet apparatus; antibacterial activity, minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs) of the active extract were evaluated by standard methods against clinically isolated drug-resistant E. coli and K. pneumoniae; preliminary phytochemical screening was taken to detect the presence of alkaloids, saponins, flavonoids, steroids, tannins, reducing sugars, phenolics, protein, and oil and fat; and bioactive compounds were detected by GCMS analysis of the active extracts. Determination of antibacterial activity showed that the test organisms were susceptible to methanol and aqueous extracts only. MIC of methanolic extract was found to be 20 µg/mL on both E. coli and K. pneumoniae, while aqueous extract had MIC of 10 and 20 µg/mL on E. coli and K. pneumoniae, respectively. Preliminary phytochemical screening showed the presence of all the above-mentioned phytochemicals except oil and fat. The significance of this work is to find a lasting solution to the current problem of emerging drug-resistant bacteria (E. coli and K. pneumoniae) through the use of extracts obtained from P. indica which have long history of use as traditional medicine. The methanolic and aqueous extract can be recommended as an alternative and candidates for drug development against drug-resistant E. coli and K. pneumoniae.
format	article
author	Bashir Salim Faruk Kumar Gaurav
author_facet	Bashir Salim Faruk Kumar Gaurav
author_sort	Bashir Salim Faruk
title	Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae
title_short	Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae
title_full	Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae
title_fullStr	Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae
title_full_unstemmed	Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae
title_sort	preliminary phytochemical screening and in vitro antibacterial activity of plumbago indica (laal chitrak) root extracts against drug-resistant escherichia coli and klebsiella pneumoniae
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/496b9314bc6449a794af05d53875f5e9
work_keys_str_mv	AT bashirsalimfaruk preliminaryphytochemicalscreeningandinvitroantibacterialactivityofplumbagoindicalaalchitrakrootextractsagainstdrugresistantescherichiacoliandklebsiellapneumoniae AT kumargaurav preliminaryphytochemicalscreeningandinvitroantibacterialactivityofplumbagoindicalaalchitrakrootextractsagainstdrugresistantescherichiacoliandklebsiellapneumoniae
_version_	1718371532676268032

Preliminary phytochemical screening and in vitro antibacterial activity of Plumbago indica (Laal chitrak) root extracts against drug-resistant Escherichia coli and Klebsiella pneumoniae

Ejemplares similares