Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development

The use of assisted reproductive technologies (ART) still requires strategies through which to maximize individual fertility chances. In vitro folliculogenesis (<i>iv</i>F) may represent a valid option to convey the large source of immature oocytes in ART. Several efforts have been made...

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Autores principales:	Chiara Di Berardino, Alessia Peserico, Giulia Capacchietti, Martina Crociati, Maurizio Monaci, Umberto Tosi, Annunziata Mauro, Valentina Russo, Nicola Bernabò, Barbara Barboni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	in vitro follicle culture follicle growth eCG FSH steroidogenesis oocyte meiotic competence Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/496f36f1093b4b57b84cb918ed12d7a3
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spelling	oai:doaj.org-article:496f36f1093b4b57b84cb918ed12d7a32021-11-25T17:56:30ZEquine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development10.3390/ijms2222124221422-00671661-6596https://doaj.org/article/496f36f1093b4b57b84cb918ed12d7a32021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12422https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The use of assisted reproductive technologies (ART) still requires strategies through which to maximize individual fertility chances. In vitro folliculogenesis (<i>iv</i>F) may represent a valid option to convey the large source of immature oocytes in ART. Several efforts have been made to set up <i>iv</i>F cultural protocols in medium-sized mammals, starting with the identification of the most suitable gonadotropic stimulus. In this study, Equine Chorionic Gonadotropin (eCG) is proposed as an alternative to Follicle Stimulating Hormone (FSH) based on its long superovulation use, trans-species validation, long half-life, and low costs. The use of 3D <i>iv</i>F on single-ovine preantral (PA) follicles allowed us to compare the hormonal effects and to validate their influence under two different cultural conditions. The use of eCG helped to stimulate the in vitro growth of ovine PA follicles by maximizing its influence under FBS-free medium. Higher performance of follicular growth, antrum formation, steroidogenic activity and gap junction marker expression were recorded. In addition, eCG, promoted a positive effect on the germinal compartment, leading to a higher incidence of meiotic competent oocytes. These findings should help to widen the use of eCG to <i>iv</i>F as a valid and largely available hormonal support enabling a synchronized in vitro follicle and oocyte development.Chiara Di BerardinoAlessia PesericoGiulia CapacchiettiMartina CrociatiMaurizio MonaciUmberto TosiAnnunziata MauroValentina RussoNicola BernabòBarbara BarboniMDPI AGarticlein vitro follicle culturefollicle growtheCGFSHsteroidogenesisoocyte meiotic competenceBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12422, p 12422 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vitro follicle culture follicle growth eCG FSH steroidogenesis oocyte meiotic competence Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	in vitro follicle culture follicle growth eCG FSH steroidogenesis oocyte meiotic competence Biology (General) QH301-705.5 Chemistry QD1-999 Chiara Di Berardino Alessia Peserico Giulia Capacchietti Martina Crociati Maurizio Monaci Umberto Tosi Annunziata Mauro Valentina Russo Nicola Bernabò Barbara Barboni Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development
description	The use of assisted reproductive technologies (ART) still requires strategies through which to maximize individual fertility chances. In vitro folliculogenesis (<i>iv</i>F) may represent a valid option to convey the large source of immature oocytes in ART. Several efforts have been made to set up <i>iv</i>F cultural protocols in medium-sized mammals, starting with the identification of the most suitable gonadotropic stimulus. In this study, Equine Chorionic Gonadotropin (eCG) is proposed as an alternative to Follicle Stimulating Hormone (FSH) based on its long superovulation use, trans-species validation, long half-life, and low costs. The use of 3D <i>iv</i>F on single-ovine preantral (PA) follicles allowed us to compare the hormonal effects and to validate their influence under two different cultural conditions. The use of eCG helped to stimulate the in vitro growth of ovine PA follicles by maximizing its influence under FBS-free medium. Higher performance of follicular growth, antrum formation, steroidogenic activity and gap junction marker expression were recorded. In addition, eCG, promoted a positive effect on the germinal compartment, leading to a higher incidence of meiotic competent oocytes. These findings should help to widen the use of eCG to <i>iv</i>F as a valid and largely available hormonal support enabling a synchronized in vitro follicle and oocyte development.
format	article
author	Chiara Di Berardino Alessia Peserico Giulia Capacchietti Martina Crociati Maurizio Monaci Umberto Tosi Annunziata Mauro Valentina Russo Nicola Bernabò Barbara Barboni
author_facet	Chiara Di Berardino Alessia Peserico Giulia Capacchietti Martina Crociati Maurizio Monaci Umberto Tosi Annunziata Mauro Valentina Russo Nicola Bernabò Barbara Barboni
author_sort	Chiara Di Berardino
title	Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development
title_short	Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development
title_full	Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development
title_fullStr	Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development
title_full_unstemmed	Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development
title_sort	equine chorionic gonadotropin as an effective fsh replacement for in vitro ovine follicle and oocyte development
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/496f36f1093b4b57b84cb918ed12d7a3
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Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development

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