Real-time monitoring of cisplatin-induced cell death.

Since the discovery of cisplatin more than 40 years ago and its clinical introduction in the 1970s an enormous amount of research has gone into elucidating the mechanism of action of cisplatin on tumor cells. With a novel cell biosensor chip system allowing continuous monitoring of respiration, glyc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hamed Alborzinia, Suzan Can, Pavlo Holenya, Catharina Scholl, Elke Lederer, Igor Kitanovic, Stefan Wölfl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/496faa8e06dc40e1b47cdef474b3182c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:496faa8e06dc40e1b47cdef474b3182c
record_format	dspace
spelling	oai:doaj.org-article:496faa8e06dc40e1b47cdef474b3182c2021-11-18T06:53:57ZReal-time monitoring of cisplatin-induced cell death.1932-620310.1371/journal.pone.0019714https://doaj.org/article/496faa8e06dc40e1b47cdef474b3182c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21603599/?tool=EBIhttps://doaj.org/toc/1932-6203Since the discovery of cisplatin more than 40 years ago and its clinical introduction in the 1970s an enormous amount of research has gone into elucidating the mechanism of action of cisplatin on tumor cells. With a novel cell biosensor chip system allowing continuous monitoring of respiration, glycolysis, and impedance we followed cisplatin treatment of different cancer cell lines in real-time. Our measurements reveal a first effect on respiration, in all cisplatin treated cell lines, followed with a significant delay by interference with glycolysis in HT-29, HCT-116, HepG2, and MCF-7 cells but not in the cisplatin-resistant cell line MDA-MB-231. Most strikingly, cell death started in all cisplatin-sensitive cell lines within 8 to 11 h of treatment, indicating a clear time frame from exposure, first response to cisplatin lesions, to cell fate decision. The time points of most significant changes were selected for more detailed analysis of cisplatin response in the breast cancer cell line MCF-7. Phosphorylation of selected signal transduction mediators connected with cellular proliferation, as well as changes in gene expression, were analyzed in samples obtained directly from sensor chips at the time points when changes in glycolysis and impedance occurred. Our online cell biosensor measurements reveal for the first time the time scale of metabolic response until onset of cell death under cisplatin treatment, which is in good agreement with models of p53-mediated cell fate decision.Hamed AlborziniaSuzan CanPavlo HolenyaCatharina SchollElke LedererIgor KitanovicStefan WölflPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19714 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamed Alborzinia Suzan Can Pavlo Holenya Catharina Scholl Elke Lederer Igor Kitanovic Stefan Wölfl Real-time monitoring of cisplatin-induced cell death.
description	Since the discovery of cisplatin more than 40 years ago and its clinical introduction in the 1970s an enormous amount of research has gone into elucidating the mechanism of action of cisplatin on tumor cells. With a novel cell biosensor chip system allowing continuous monitoring of respiration, glycolysis, and impedance we followed cisplatin treatment of different cancer cell lines in real-time. Our measurements reveal a first effect on respiration, in all cisplatin treated cell lines, followed with a significant delay by interference with glycolysis in HT-29, HCT-116, HepG2, and MCF-7 cells but not in the cisplatin-resistant cell line MDA-MB-231. Most strikingly, cell death started in all cisplatin-sensitive cell lines within 8 to 11 h of treatment, indicating a clear time frame from exposure, first response to cisplatin lesions, to cell fate decision. The time points of most significant changes were selected for more detailed analysis of cisplatin response in the breast cancer cell line MCF-7. Phosphorylation of selected signal transduction mediators connected with cellular proliferation, as well as changes in gene expression, were analyzed in samples obtained directly from sensor chips at the time points when changes in glycolysis and impedance occurred. Our online cell biosensor measurements reveal for the first time the time scale of metabolic response until onset of cell death under cisplatin treatment, which is in good agreement with models of p53-mediated cell fate decision.
format	article
author	Hamed Alborzinia Suzan Can Pavlo Holenya Catharina Scholl Elke Lederer Igor Kitanovic Stefan Wölfl
author_facet	Hamed Alborzinia Suzan Can Pavlo Holenya Catharina Scholl Elke Lederer Igor Kitanovic Stefan Wölfl
author_sort	Hamed Alborzinia
title	Real-time monitoring of cisplatin-induced cell death.
title_short	Real-time monitoring of cisplatin-induced cell death.
title_full	Real-time monitoring of cisplatin-induced cell death.
title_fullStr	Real-time monitoring of cisplatin-induced cell death.
title_full_unstemmed	Real-time monitoring of cisplatin-induced cell death.
title_sort	real-time monitoring of cisplatin-induced cell death.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/496faa8e06dc40e1b47cdef474b3182c
work_keys_str_mv	AT hamedalborzinia realtimemonitoringofcisplatininducedcelldeath AT suzancan realtimemonitoringofcisplatininducedcelldeath AT pavloholenya realtimemonitoringofcisplatininducedcelldeath AT catharinascholl realtimemonitoringofcisplatininducedcelldeath AT elkelederer realtimemonitoringofcisplatininducedcelldeath AT igorkitanovic realtimemonitoringofcisplatininducedcelldeath AT stefanwolfl realtimemonitoringofcisplatininducedcelldeath
_version_	1718424226676867072

Real-time monitoring of cisplatin-induced cell death.

Ejemplares similares