Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence

Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of...

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Autores principales:	Anja M. Richter, Gerda Buchberger, David Stifter, Jiri Duchoslav, Andreas Hertwig, Jörn Bonse, Johannes Heitz, Karin Schwibbert
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	laser-induced periodic surface structures (LIPSS) laser processing polyethylene terephthalate biofilm formation cell appendages F pili Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4971443b5a62419986f6562699a6f423
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spelling	oai:doaj.org-article:4971443b5a62419986f6562699a6f4232021-11-25T18:31:33ZSpatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence10.3390/nano111130002079-4991https://doaj.org/article/4971443b5a62419986f6562699a6f4232021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3000https://doaj.org/toc/2079-4991Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns), laser-induced periodic surface structures (LIPSS) featuring different sub-micrometric periods ranging from ~210 to ~610 nm were processed on commercial poly(ethylene terephthalate) (PET) foils. Bacterial adhesion tests revealed that these nanorippled surfaces exhibit a repellence for <i>E. coli</i> that decisively depends on the spatial periods of the LIPSS with the strongest reduction (~91%) in cell adhesion observed for LIPSS periods of 214 nm. Although chemical and structural analyses indicated a moderate laser-induced surface oxidation, a significant influence on the bacterial adhesion was ruled out. Scanning electron microscopy and additional biofilm studies using a pili-deficient <i>E. coli</i> TG1 strain revealed the role of extracellular appendages in the bacterial repellence observed here.Anja M. RichterGerda BuchbergerDavid StifterJiri DuchoslavAndreas HertwigJörn BonseJohannes HeitzKarin SchwibbertMDPI AGarticlelaser-induced periodic surface structures (LIPSS)laser processingpolyethylene terephthalatebiofilm formationcell appendagesF piliChemistryQD1-999ENNanomaterials, Vol 11, Iss 3000, p 3000 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laser-induced periodic surface structures (LIPSS) laser processing polyethylene terephthalate biofilm formation cell appendages F pili Chemistry QD1-999
spellingShingle	laser-induced periodic surface structures (LIPSS) laser processing polyethylene terephthalate biofilm formation cell appendages F pili Chemistry QD1-999 Anja M. Richter Gerda Buchberger David Stifter Jiri Duchoslav Andreas Hertwig Jörn Bonse Johannes Heitz Karin Schwibbert Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence
description	Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns), laser-induced periodic surface structures (LIPSS) featuring different sub-micrometric periods ranging from ~210 to ~610 nm were processed on commercial poly(ethylene terephthalate) (PET) foils. Bacterial adhesion tests revealed that these nanorippled surfaces exhibit a repellence for <i>E. coli</i> that decisively depends on the spatial periods of the LIPSS with the strongest reduction (~91%) in cell adhesion observed for LIPSS periods of 214 nm. Although chemical and structural analyses indicated a moderate laser-induced surface oxidation, a significant influence on the bacterial adhesion was ruled out. Scanning electron microscopy and additional biofilm studies using a pili-deficient <i>E. coli</i> TG1 strain revealed the role of extracellular appendages in the bacterial repellence observed here.
format	article
author	Anja M. Richter Gerda Buchberger David Stifter Jiri Duchoslav Andreas Hertwig Jörn Bonse Johannes Heitz Karin Schwibbert
author_facet	Anja M. Richter Gerda Buchberger David Stifter Jiri Duchoslav Andreas Hertwig Jörn Bonse Johannes Heitz Karin Schwibbert
author_sort	Anja M. Richter
title	Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence
title_short	Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence
title_full	Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence
title_fullStr	Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence
title_full_unstemmed	Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence
title_sort	spatial period of laser-induced surface nanoripples on pet determines <i>escherichia coli</i> repellence
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4971443b5a62419986f6562699a6f423
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Spatial Period of Laser-Induced Surface Nanoripples on PET Determines <i>Escherichia coli</i> Repellence

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