Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection

Monocytes, one of the main target cells for dengue virus (DENV) infection, contribute to the resolution of viremia and to pathogenesis. We performed a longitudinal study by a detailed phenotypic comparison of classical (CD14++CD16−, non-classical (CD14+CD16++) and intermediate (CD14++CD16+) monocyte...

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Autores principales:	Sakaorat Lertjuthaporn, Rassamon Keawvichit, Korakot Polsrila, Kasama Sukapirom, Ampaiwan Chuansumrit, Kulkanya Chokephaibulkit, Aftab A. Ansari, Ladawan Khowawisetsut, Kovit Pattanapanyasat
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dengue infection monocyte flow cytometry co-stimulation Medicine R
Acceso en línea:	https://doaj.org/article/497cad51658c43059e01617982a88820
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spelling	oai:doaj.org-article:497cad51658c43059e01617982a888202021-11-25T18:38:24ZKinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection10.3390/pathogens101114582076-0817https://doaj.org/article/497cad51658c43059e01617982a888202021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1458https://doaj.org/toc/2076-0817Monocytes, one of the main target cells for dengue virus (DENV) infection, contribute to the resolution of viremia and to pathogenesis. We performed a longitudinal study by a detailed phenotypic comparison of classical (CD14++CD16−, non-classical (CD14+CD16++) and intermediate (CD14++CD16+) monocyte subsets in blood samples from dengue fever (DF) to the severe dengue hemorrhagic fever (DHF) and healthy individuals. Various costimulatory molecules of CD40, CD80, CD86 and inducible costimulatory ligand (ICOSL) expressed on these three monocyte subsets were also analyzed. DENV-infected patients showed an increase in the frequency of intermediate monocytes and a decrease in the classical monocytes when compared to healthy individuals. Although these differences did not correlate with disease severity, changes during the early phase of infection gradually returned to normal in the defervescence phase. Moreover, decreased frequency of classical monocytes was associated with a significant up-regulation of co-stimulatory molecules CD40, CD86 and ICOSL. Kinetics of these co-stimulatory molecule-expressing classical monocytes showed different patterns throughout the sampling times of acute DENV infection. Different distribution of monocyte subsets and their co-stimulatory molecules in the peripheral blood during acute infection might exacerbate immune responses like cytokine storms and ADE, and future studies on intracellular molecular pathways utilized by these monocyte linages are warranted.Sakaorat LertjuthapornRassamon KeawvichitKorakot PolsrilaKasama SukapiromAmpaiwan ChuansumritKulkanya ChokephaibulkitAftab A. AnsariLadawan KhowawisetsutKovit PattanapanyasatMDPI AGarticledengue infectionmonocyteflow cytometryco-stimulationMedicineRENPathogens, Vol 10, Iss 1458, p 1458 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dengue infection monocyte flow cytometry co-stimulation Medicine R
spellingShingle	dengue infection monocyte flow cytometry co-stimulation Medicine R Sakaorat Lertjuthaporn Rassamon Keawvichit Korakot Polsrila Kasama Sukapirom Ampaiwan Chuansumrit Kulkanya Chokephaibulkit Aftab A. Ansari Ladawan Khowawisetsut Kovit Pattanapanyasat Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection
description	Monocytes, one of the main target cells for dengue virus (DENV) infection, contribute to the resolution of viremia and to pathogenesis. We performed a longitudinal study by a detailed phenotypic comparison of classical (CD14++CD16−, non-classical (CD14+CD16++) and intermediate (CD14++CD16+) monocyte subsets in blood samples from dengue fever (DF) to the severe dengue hemorrhagic fever (DHF) and healthy individuals. Various costimulatory molecules of CD40, CD80, CD86 and inducible costimulatory ligand (ICOSL) expressed on these three monocyte subsets were also analyzed. DENV-infected patients showed an increase in the frequency of intermediate monocytes and a decrease in the classical monocytes when compared to healthy individuals. Although these differences did not correlate with disease severity, changes during the early phase of infection gradually returned to normal in the defervescence phase. Moreover, decreased frequency of classical monocytes was associated with a significant up-regulation of co-stimulatory molecules CD40, CD86 and ICOSL. Kinetics of these co-stimulatory molecule-expressing classical monocytes showed different patterns throughout the sampling times of acute DENV infection. Different distribution of monocyte subsets and their co-stimulatory molecules in the peripheral blood during acute infection might exacerbate immune responses like cytokine storms and ADE, and future studies on intracellular molecular pathways utilized by these monocyte linages are warranted.
format	article
author	Sakaorat Lertjuthaporn Rassamon Keawvichit Korakot Polsrila Kasama Sukapirom Ampaiwan Chuansumrit Kulkanya Chokephaibulkit Aftab A. Ansari Ladawan Khowawisetsut Kovit Pattanapanyasat
author_facet	Sakaorat Lertjuthaporn Rassamon Keawvichit Korakot Polsrila Kasama Sukapirom Ampaiwan Chuansumrit Kulkanya Chokephaibulkit Aftab A. Ansari Ladawan Khowawisetsut Kovit Pattanapanyasat
author_sort	Sakaorat Lertjuthaporn
title	Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection
title_short	Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection
title_full	Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection
title_fullStr	Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection
title_full_unstemmed	Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection
title_sort	kinetic changes of peripheral blood monocyte subsets and expression of co-stimulatory molecules during acute dengue virus infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/497cad51658c43059e01617982a88820
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Kinetic Changes of Peripheral Blood Monocyte Subsets and Expression of Co-Stimulatory Molecules during Acute Dengue Virus Infection

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