Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability

Abstract E3 ligases play significant roles in plant stress tolerance by targeting specific substrate proteins for post-translational modification. In a previous study, we cloned TaPUB1 from Triticum aestivum L., which encodes a U-box E3 ligase. Real-time polymerase chain reaction revealed that the g...

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Autores principales:	Guangqiang Zhang, Meng Zhang, Zhongxian Zhao, Yuanqing Ren, Qinxue Li, Wei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4982d55301f34bdc9500df550637b128
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spelling	oai:doaj.org-article:4982d55301f34bdc9500df550637b1282021-12-02T16:06:11ZWheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability10.1038/s41598-017-08181-w2045-2322https://doaj.org/article/4982d55301f34bdc9500df550637b1282017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08181-whttps://doaj.org/toc/2045-2322Abstract E3 ligases play significant roles in plant stress tolerance by targeting specific substrate proteins for post-translational modification. In a previous study, we cloned TaPUB1 from Triticum aestivum L., which encodes a U-box E3 ligase. Real-time polymerase chain reaction revealed that the gene was up-regulated under drought stress. To investigate the function of TaPUB1 in the response of plants to drought, we generated transgenic Nicotiana benthamiana (N. benthamiana) plants constitutively expressing TaPUB1 under the CaMV35S promoter. Compared to wild type (WT), the transgenic plants had higher germination and seedling survival rates as well as higher photosynthetic rate and water retention, suggesting that the overexpression of TaPUB1 enhanced the drought tolerance of the TaPUB1 overexpressing (OE) plants. Moreover, less accumulation of reactive oxygen species (ROS) and stronger antioxidant capacity were detected in the OE plants than in the WT plants. To characterize the mechanisms involved, methyl viologen (MV) was used to induce oxidative stress conditions and we identified the functions of this gene in the plant tolerance to oxidative stress. Our results suggest that TaPUB1 positively modulates plant drought stress resistance potential by improving their antioxidant capacity.Guangqiang ZhangMeng ZhangZhongxian ZhaoYuanqing RenQinxue LiWei WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guangqiang Zhang Meng Zhang Zhongxian Zhao Yuanqing Ren Qinxue Li Wei Wang Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability
description	Abstract E3 ligases play significant roles in plant stress tolerance by targeting specific substrate proteins for post-translational modification. In a previous study, we cloned TaPUB1 from Triticum aestivum L., which encodes a U-box E3 ligase. Real-time polymerase chain reaction revealed that the gene was up-regulated under drought stress. To investigate the function of TaPUB1 in the response of plants to drought, we generated transgenic Nicotiana benthamiana (N. benthamiana) plants constitutively expressing TaPUB1 under the CaMV35S promoter. Compared to wild type (WT), the transgenic plants had higher germination and seedling survival rates as well as higher photosynthetic rate and water retention, suggesting that the overexpression of TaPUB1 enhanced the drought tolerance of the TaPUB1 overexpressing (OE) plants. Moreover, less accumulation of reactive oxygen species (ROS) and stronger antioxidant capacity were detected in the OE plants than in the WT plants. To characterize the mechanisms involved, methyl viologen (MV) was used to induce oxidative stress conditions and we identified the functions of this gene in the plant tolerance to oxidative stress. Our results suggest that TaPUB1 positively modulates plant drought stress resistance potential by improving their antioxidant capacity.
format	article
author	Guangqiang Zhang Meng Zhang Zhongxian Zhao Yuanqing Ren Qinxue Li Wei Wang
author_facet	Guangqiang Zhang Meng Zhang Zhongxian Zhao Yuanqing Ren Qinxue Li Wei Wang
author_sort	Guangqiang Zhang
title	Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability
title_short	Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability
title_full	Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability
title_fullStr	Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability
title_full_unstemmed	Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability
title_sort	wheat tapub1 modulates plant drought stress resistance by improving antioxidant capability
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4982d55301f34bdc9500df550637b128
work_keys_str_mv	AT guangqiangzhang wheattapub1modulatesplantdroughtstressresistancebyimprovingantioxidantcapability AT mengzhang wheattapub1modulatesplantdroughtstressresistancebyimprovingantioxidantcapability AT zhongxianzhao wheattapub1modulatesplantdroughtstressresistancebyimprovingantioxidantcapability AT yuanqingren wheattapub1modulatesplantdroughtstressresistancebyimprovingantioxidantcapability AT qinxueli wheattapub1modulatesplantdroughtstressresistancebyimprovingantioxidantcapability AT weiwang wheattapub1modulatesplantdroughtstressresistancebyimprovingantioxidantcapability
_version_	1718385085631168512

Wheat TaPUB1 modulates plant drought stress resistance by improving antioxidant capability

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