Transcriptome analysis of bread wheat leaves in response to salt stress.

Salinity is one of the main abiotic stresses limiting crop productivity. In the current study, the transcriptome of wheat leaves in an Iranian salt-tolerant cultivar (Arg) was investigated in response to salinity stress to identify salinity stress-responsive genes and mechanisms. More than 114 milli...

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Autores principales:	Nazanin Amirbakhtiar, Ahmad Ismaili, Mohammad-Reza Ghaffari, Raheleh Mirdar Mansuri, Sepideh Sanjari, Zahra-Sadat Shobbar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4985835462c04ff1bbe1002df260497a
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spelling	oai:doaj.org-article:4985835462c04ff1bbe1002df260497a2021-12-02T20:09:22ZTranscriptome analysis of bread wheat leaves in response to salt stress.1932-620310.1371/journal.pone.0254189https://doaj.org/article/4985835462c04ff1bbe1002df260497a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254189https://doaj.org/toc/1932-6203Salinity is one of the main abiotic stresses limiting crop productivity. In the current study, the transcriptome of wheat leaves in an Iranian salt-tolerant cultivar (Arg) was investigated in response to salinity stress to identify salinity stress-responsive genes and mechanisms. More than 114 million reads were generated from leaf tissues by the Illumina HiSeq 2500 platform. An amount of 81.9% to 85.7% of reads could be mapped to the wheat reference genome for different samples. The data analysis led to the identification of 98819 genes, including 26700 novel transcripts. A total of 4290 differentially expressed genes (DEGs) were recognized, comprising 2346 up-regulated genes and 1944 down-regulated genes. Clustering of the DEGs utilizing Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that transcripts associated with phenylpropanoid biosynthesis, transporters, transcription factors, hormone signal transduction, glycosyltransferases, exosome, and MAPK signaling might be involved in salt tolerance. The expression patterns of nine DEGs were investigated by quantitative real-time PCR in Arg and Moghan3 as the salt-tolerant and susceptible cultivars, respectively. The obtained results were consistent with changes in transcript abundance found by RNA-sequencing in the tolerant cultivar. The results presented here could be utilized for salt tolerance enhancement in wheat through genetic engineering or molecular breeding.Nazanin AmirbakhtiarAhmad IsmailiMohammad-Reza GhaffariRaheleh Mirdar MansuriSepideh SanjariZahra-Sadat ShobbarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254189 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nazanin Amirbakhtiar Ahmad Ismaili Mohammad-Reza Ghaffari Raheleh Mirdar Mansuri Sepideh Sanjari Zahra-Sadat Shobbar Transcriptome analysis of bread wheat leaves in response to salt stress.
description	Salinity is one of the main abiotic stresses limiting crop productivity. In the current study, the transcriptome of wheat leaves in an Iranian salt-tolerant cultivar (Arg) was investigated in response to salinity stress to identify salinity stress-responsive genes and mechanisms. More than 114 million reads were generated from leaf tissues by the Illumina HiSeq 2500 platform. An amount of 81.9% to 85.7% of reads could be mapped to the wheat reference genome for different samples. The data analysis led to the identification of 98819 genes, including 26700 novel transcripts. A total of 4290 differentially expressed genes (DEGs) were recognized, comprising 2346 up-regulated genes and 1944 down-regulated genes. Clustering of the DEGs utilizing Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that transcripts associated with phenylpropanoid biosynthesis, transporters, transcription factors, hormone signal transduction, glycosyltransferases, exosome, and MAPK signaling might be involved in salt tolerance. The expression patterns of nine DEGs were investigated by quantitative real-time PCR in Arg and Moghan3 as the salt-tolerant and susceptible cultivars, respectively. The obtained results were consistent with changes in transcript abundance found by RNA-sequencing in the tolerant cultivar. The results presented here could be utilized for salt tolerance enhancement in wheat through genetic engineering or molecular breeding.
format	article
author	Nazanin Amirbakhtiar Ahmad Ismaili Mohammad-Reza Ghaffari Raheleh Mirdar Mansuri Sepideh Sanjari Zahra-Sadat Shobbar
author_facet	Nazanin Amirbakhtiar Ahmad Ismaili Mohammad-Reza Ghaffari Raheleh Mirdar Mansuri Sepideh Sanjari Zahra-Sadat Shobbar
author_sort	Nazanin Amirbakhtiar
title	Transcriptome analysis of bread wheat leaves in response to salt stress.
title_short	Transcriptome analysis of bread wheat leaves in response to salt stress.
title_full	Transcriptome analysis of bread wheat leaves in response to salt stress.
title_fullStr	Transcriptome analysis of bread wheat leaves in response to salt stress.
title_full_unstemmed	Transcriptome analysis of bread wheat leaves in response to salt stress.
title_sort	transcriptome analysis of bread wheat leaves in response to salt stress.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4985835462c04ff1bbe1002df260497a
work_keys_str_mv	AT nazaninamirbakhtiar transcriptomeanalysisofbreadwheatleavesinresponsetosaltstress AT ahmadismaili transcriptomeanalysisofbreadwheatleavesinresponsetosaltstress AT mohammadrezaghaffari transcriptomeanalysisofbreadwheatleavesinresponsetosaltstress AT rahelehmirdarmansuri transcriptomeanalysisofbreadwheatleavesinresponsetosaltstress AT sepidehsanjari transcriptomeanalysisofbreadwheatleavesinresponsetosaltstress AT zahrasadatshobbar transcriptomeanalysisofbreadwheatleavesinresponsetosaltstress
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Transcriptome analysis of bread wheat leaves in response to salt stress.

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