Capability of tip-growing plant cells to penetrate into extremely narrow gaps

Abstract Plant cells are covered with rigid cell walls, yet tip-growing cells can elongate by providing new cell wall material to their apical regions. Studies of the mechanical properties of tip-growing plant cells typically involve measurement of the turgor pressure and stiffness of the cells’ api...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Naoki Yanagisawa, Nagisa Sugimoto, Hideyuki Arata, Tetsuya Higashiyama, Yoshikatsu Sato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4987615b7ac7429982e6343e490f5c31
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4987615b7ac7429982e6343e490f5c31
record_format	dspace
spelling	oai:doaj.org-article:4987615b7ac7429982e6343e490f5c312021-12-02T15:06:19ZCapability of tip-growing plant cells to penetrate into extremely narrow gaps10.1038/s41598-017-01610-w2045-2322https://doaj.org/article/4987615b7ac7429982e6343e490f5c312017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01610-whttps://doaj.org/toc/2045-2322Abstract Plant cells are covered with rigid cell walls, yet tip-growing cells can elongate by providing new cell wall material to their apical regions. Studies of the mechanical properties of tip-growing plant cells typically involve measurement of the turgor pressure and stiffness of the cells’ apical regions. These experiments, however, do not address how living tip-growing cells react when they encounter physical obstacles that are not substantially altered by turgor pressure. To investigate this issue, we constructed microfabricated platforms with a series of artificial gaps as small as 1 μm, and examined the capability of tip-growing plant cells, including pollen tubes, root hairs, and moss protonemata, to penetrate into these gaps. The cells were grown inside microfluidic chambers and guided towards the gaps using microdevices customized for each cell type. All types of tip-growing cells could grow through the microgaps with their organelles intact, even though the gaps were much smaller than the cylindrical cell diameter. Our findings reveal the dramatic physiological and developmental flexibility of tip-growing plant cells. The microfluidic platforms designed in this study provide novel tools for the elucidation of the mechanical properties of tip-growing plant cells in extremely small spaces.Naoki YanagisawaNagisa SugimotoHideyuki ArataTetsuya HigashiyamaYoshikatsu SatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Naoki Yanagisawa Nagisa Sugimoto Hideyuki Arata Tetsuya Higashiyama Yoshikatsu Sato Capability of tip-growing plant cells to penetrate into extremely narrow gaps
description	Abstract Plant cells are covered with rigid cell walls, yet tip-growing cells can elongate by providing new cell wall material to their apical regions. Studies of the mechanical properties of tip-growing plant cells typically involve measurement of the turgor pressure and stiffness of the cells’ apical regions. These experiments, however, do not address how living tip-growing cells react when they encounter physical obstacles that are not substantially altered by turgor pressure. To investigate this issue, we constructed microfabricated platforms with a series of artificial gaps as small as 1 μm, and examined the capability of tip-growing plant cells, including pollen tubes, root hairs, and moss protonemata, to penetrate into these gaps. The cells were grown inside microfluidic chambers and guided towards the gaps using microdevices customized for each cell type. All types of tip-growing cells could grow through the microgaps with their organelles intact, even though the gaps were much smaller than the cylindrical cell diameter. Our findings reveal the dramatic physiological and developmental flexibility of tip-growing plant cells. The microfluidic platforms designed in this study provide novel tools for the elucidation of the mechanical properties of tip-growing plant cells in extremely small spaces.
format	article
author	Naoki Yanagisawa Nagisa Sugimoto Hideyuki Arata Tetsuya Higashiyama Yoshikatsu Sato
author_facet	Naoki Yanagisawa Nagisa Sugimoto Hideyuki Arata Tetsuya Higashiyama Yoshikatsu Sato
author_sort	Naoki Yanagisawa
title	Capability of tip-growing plant cells to penetrate into extremely narrow gaps
title_short	Capability of tip-growing plant cells to penetrate into extremely narrow gaps
title_full	Capability of tip-growing plant cells to penetrate into extremely narrow gaps
title_fullStr	Capability of tip-growing plant cells to penetrate into extremely narrow gaps
title_full_unstemmed	Capability of tip-growing plant cells to penetrate into extremely narrow gaps
title_sort	capability of tip-growing plant cells to penetrate into extremely narrow gaps
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4987615b7ac7429982e6343e490f5c31
work_keys_str_mv	AT naokiyanagisawa capabilityoftipgrowingplantcellstopenetrateintoextremelynarrowgaps AT nagisasugimoto capabilityoftipgrowingplantcellstopenetrateintoextremelynarrowgaps AT hideyukiarata capabilityoftipgrowingplantcellstopenetrateintoextremelynarrowgaps AT tetsuyahigashiyama capabilityoftipgrowingplantcellstopenetrateintoextremelynarrowgaps AT yoshikatsusato capabilityoftipgrowingplantcellstopenetrateintoextremelynarrowgaps
_version_	1718388509154213888

Capability of tip-growing plant cells to penetrate into extremely narrow gaps

Ejemplares similares