The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis
Yi Zhou Department of Endocrinology, Xiamen Hospital, Beijing University of Traditional Chinese Medicine (Xiamen Hospital of Traditional Chinese Medicine), Xiamen, Fujian 361008, People’s Republic of ChinaCorrespondence: Yi ZhouDepartment of Endocrinology, Xiamen Hospital, Beijing Universi...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/49894577a42648b4a244793c2e6cf7fb |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:49894577a42648b4a244793c2e6cf7fb |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:49894577a42648b4a244793c2e6cf7fb2021-12-02T15:21:58ZThe Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis1178-7007https://doaj.org/article/49894577a42648b4a244793c2e6cf7fb2020-06-01T00:00:00Zhttps://www.dovepress.com/the-protective-effects-of-cryptochlorogenic-acid-on-beta-cells-functio-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Yi Zhou Department of Endocrinology, Xiamen Hospital, Beijing University of Traditional Chinese Medicine (Xiamen Hospital of Traditional Chinese Medicine), Xiamen, Fujian 361008, People’s Republic of ChinaCorrespondence: Yi ZhouDepartment of Endocrinology, Xiamen Hospital, Beijing University of Traditional Chinese Medicine (Xiamen Hospital of Traditional Chinese Medicine), 1739, Xianyue Road, Huli District, Xiamen City, Fujian Province, People’s Republic of ChinaEmail yizhoufj@163.comPurpose: Mulberry leaf extract has exerted better antidiabetic activities, while the effects of major active components in mulberry leaf extract are still unclear. Cryptochlorogenic acid (CCA) as the major active component in mulberry leaf extracts was investigated herein.Materials and Methods: Rats were treated with 50mg/kg streptozotocin for the establishment of diabetic model in vivo, and cells were treated with 33.3 mM glucose for the establishment of cell model in vitro. HE staining assay was performed for observation of pancreatic pathology and aldehyde fuchsin staining assay for examining islet cell numbers. The iron content was detected via Perls staining assay with iron assay kit (ab83366). The malondialdehyde (MDA), glutathione (GSH) and oxidized glutathione (GSSG) were detected by corresponding kits. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed for assessment of gene level and Western blot for measurement of protein expression level. The cell survival was detected via CCK-8 assay.Results: The blood glucose level, iron content, accumulation of lipid peroxides and islet injury in diabetic model were all improved by CCA via a concentration-dependent manner. CCA functions via inhibition of ferroptosis by activation of cystine/glutamate transporter system (XC−)/glutathione peroxidase 4(GPX4)/Nrf2 and inhibition of nuclear receptor coactivator 4 (NCOA4) in diabetes.Conclusion: CCA exerted excellent antidiabetic effects via inhibition of ferroptosis, so it may be a promising agent for diabetes therapy, providing a new avenue for diabetes treatment.Keywords: cryptochlorogenic acid, diabetes, XC-/GPX4, ferroptosis, Nrf2, NCOA4Zhou YDove Medical Pressarticlecryptochlorogenic acid，diabetes， xc-/gpx4 ，ferroptosis，nrf2，ncoa4Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 1921-1931 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
cryptochlorogenic acid，diabetes， xc-/gpx4 ，ferroptosis，nrf2，ncoa4 Specialties of internal medicine RC581-951 |
| spellingShingle |
cryptochlorogenic acid，diabetes， xc-/gpx4 ，ferroptosis，nrf2，ncoa4 Specialties of internal medicine RC581-951 Zhou Y The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis |
| description |
Yi Zhou Department of Endocrinology, Xiamen Hospital, Beijing University of Traditional Chinese Medicine (Xiamen Hospital of Traditional Chinese Medicine), Xiamen, Fujian 361008, People’s Republic of ChinaCorrespondence: Yi ZhouDepartment of Endocrinology, Xiamen Hospital, Beijing University of Traditional Chinese Medicine (Xiamen Hospital of Traditional Chinese Medicine), 1739, Xianyue Road, Huli District, Xiamen City, Fujian Province, People’s Republic of ChinaEmail yizhoufj@163.comPurpose: Mulberry leaf extract has exerted better antidiabetic activities, while the effects of major active components in mulberry leaf extract are still unclear. Cryptochlorogenic acid (CCA) as the major active component in mulberry leaf extracts was investigated herein.Materials and Methods: Rats were treated with 50mg/kg streptozotocin for the establishment of diabetic model in vivo, and cells were treated with 33.3 mM glucose for the establishment of cell model in vitro. HE staining assay was performed for observation of pancreatic pathology and aldehyde fuchsin staining assay for examining islet cell numbers. The iron content was detected via Perls staining assay with iron assay kit (ab83366). The malondialdehyde (MDA), glutathione (GSH) and oxidized glutathione (GSSG) were detected by corresponding kits. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed for assessment of gene level and Western blot for measurement of protein expression level. The cell survival was detected via CCK-8 assay.Results: The blood glucose level, iron content, accumulation of lipid peroxides and islet injury in diabetic model were all improved by CCA via a concentration-dependent manner. CCA functions via inhibition of ferroptosis by activation of cystine/glutamate transporter system (XC−)/glutathione peroxidase 4(GPX4)/Nrf2 and inhibition of nuclear receptor coactivator 4 (NCOA4) in diabetes.Conclusion: CCA exerted excellent antidiabetic effects via inhibition of ferroptosis, so it may be a promising agent for diabetes therapy, providing a new avenue for diabetes treatment.Keywords: cryptochlorogenic acid, diabetes, XC-/GPX4, ferroptosis, Nrf2, NCOA4 |
| format |
article |
| author |
Zhou Y |
| author_facet |
Zhou Y |
| author_sort |
Zhou Y |
| title |
The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis |
| title_short |
The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis |
| title_full |
The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis |
| title_fullStr |
The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis |
| title_full_unstemmed |
The Protective Effects of Cryptochlorogenic Acid on β-Cells Function in Diabetes in vivo and vitro via Inhibition of Ferroptosis |
| title_sort |
protective effects of cryptochlorogenic acid on β-cells function in diabetes in vivo and vitro via inhibition of ferroptosis |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/49894577a42648b4a244793c2e6cf7fb |
| work_keys_str_mv |
AT zhouy theprotectiveeffectsofcryptochlorogenicacidonbetacellsfunctionindiabetesinvivoandvitroviainhibitionofferroptosis AT zhouy protectiveeffectsofcryptochlorogenicacidonbetacellsfunctionindiabetesinvivoandvitroviainhibitionofferroptosis |
| _version_ |
1718387413519171584 |