A software based application for cell proliferation determination
We evaluated a software based application for cell proliferation determination. Cells were cultured in different set-ups with and without cell-inhabitant agent. A scratch wound assay was performed to stimulate cell proliferation. The scratch wounds were photographed light-microscopically for 66.5 h....
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/498ea15145244ae0bb1595ef74b6b6bb |
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| Sumario: | We evaluated a software based application for cell proliferation determination. Cells were cultured in different set-ups with and without cell-inhabitant agent. A scratch wound assay was performed to stimulate cell proliferation. The scratch wounds were photographed light-microscopically for 66.5 h. The images were software based evaluated in timely order by comparing each image to its predecessor using a Farneback algorithm. Compared to the manual evaluation, this application is timesaving, accurate, reproducible and of objective nature independent from the observer. |
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