Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy

Maomao Hu,1,2,* Han Wang,1– 3,* Shengnan Li,1,2,* Feng Yan,2 Changning Fu,1,2 Lei Li,1,2 Yalin Yu,1,2,4 Jie Xiong,1,2 Bo Dong1,2,4 1Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250012, People’s Republic of China; 2Key Laboratory...

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Autores principales:	Hu M, Wang H, Li S, Yan F, Fu C, Li L, Yu Y, Xiong J, Dong B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	diabetic cardiomyopathy yes-associated protein myocardial fibrosis Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/4992283811d749e99197a35cea47b5f5
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spelling	oai:doaj.org-article:4992283811d749e99197a35cea47b5f52021-12-02T15:19:13ZYes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy1178-7007https://doaj.org/article/4992283811d749e99197a35cea47b5f52021-05-01T00:00:00Zhttps://www.dovepress.com/yes-associated-protein-is-involved-in-myocardial-fibrosis-in-rats-with-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Maomao Hu,1,2,* Han Wang,1– 3,* Shengnan Li,1,2,* Feng Yan,2 Changning Fu,1,2 Lei Li,1,2 Yalin Yu,1,2,4 Jie Xiong,1,2 Bo Dong1,2,4 1Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250012, People’s Republic of China; 2Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan, People’s Republic of China; 3Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, People’s Republic of China; 4Department of Cardiology, Shandong Traditional Chinese Medicine University, Jinan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bo Dong; Jie XiongDepartment of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, 250012, People’s Republic of ChinaEmail bodong@sdu.edu.cn; xiongjiede163@163.comIntroduction: Recent studies have shown that YAP is closely related to the pathological process of cardiovascular diseases. But the role of YAP in cardiac injury of diabetic cardiomyopathy (DCM) is still unclear.Methods: Diabetic cardiomyopathy rat model was established and divided into control group, DCM group, LV-SC-shRNA group and LV-YAP-shRNA group. LV-SC-shRNA group and LV-YAP-shRNA group were injected with lentivirus expressing SC-shRNA and YAP-shRNA via tail vein, respectively. Primary rat cardiac fibroblasts (CFs) were stimulated with high concentration of glucose and treated with recombinant lentivirus expressing either SC-shRNA or YAP-shRNA to observe the expression of CTGF and fibronectin, so as to observe the effect of inhibiting YAP on the pathogenesis of DCM.Results: Compared with control group, high glucose markedly increased YAP mRNA and protein expression in DCM and CFs. Inhibition of YAP decreased myocardial fibrosis and improved cardiac function in the DCM model and decreased the expression of CTGF and fibronectin in CFs. The result suggested that YAP plays a key role in the pathological progression of DCM, and the underlying mechanisms may be associated with TEAD and CTGF.Discussion: We found that the expression of YAP was increased both in vivo and in vitro, suggesting that YAP is closely related to DCM, and YAP knockdown can reduce myocardial fibrosis in rat model of DCM by reducing the expression of PAI-1, collagen I, collagen III, CTGF and profilin, as well as the expression of CTGF and fibronectin in CFs. This study revealed that YAP plays an important role in the pathological process of diabetic cardiomyopathy, and down-regulation of YAP expression may provide a new therapeutic target for DCM.Keywords: diabetic cardiomyopathy, yes-associated protein, myocardial fibrosisHu MWang HLi SYan FFu CLi LYu YXiong JDong BDove Medical Pressarticlediabetic cardiomyopathyyes-associated proteinmyocardial fibrosisSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 2133-2143 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic cardiomyopathy yes-associated protein myocardial fibrosis Specialties of internal medicine RC581-951
spellingShingle	diabetic cardiomyopathy yes-associated protein myocardial fibrosis Specialties of internal medicine RC581-951 Hu M Wang H Li S Yan F Fu C Li L Yu Y Xiong J Dong B Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy
description	Maomao Hu,1,2,* Han Wang,1– 3,* Shengnan Li,1,2,* Feng Yan,2 Changning Fu,1,2 Lei Li,1,2 Yalin Yu,1,2,4 Jie Xiong,1,2 Bo Dong1,2,4 1Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250012, People’s Republic of China; 2Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan, People’s Republic of China; 3Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, People’s Republic of China; 4Department of Cardiology, Shandong Traditional Chinese Medicine University, Jinan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bo Dong; Jie XiongDepartment of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, 250012, People’s Republic of ChinaEmail bodong@sdu.edu.cn; xiongjiede163@163.comIntroduction: Recent studies have shown that YAP is closely related to the pathological process of cardiovascular diseases. But the role of YAP in cardiac injury of diabetic cardiomyopathy (DCM) is still unclear.Methods: Diabetic cardiomyopathy rat model was established and divided into control group, DCM group, LV-SC-shRNA group and LV-YAP-shRNA group. LV-SC-shRNA group and LV-YAP-shRNA group were injected with lentivirus expressing SC-shRNA and YAP-shRNA via tail vein, respectively. Primary rat cardiac fibroblasts (CFs) were stimulated with high concentration of glucose and treated with recombinant lentivirus expressing either SC-shRNA or YAP-shRNA to observe the expression of CTGF and fibronectin, so as to observe the effect of inhibiting YAP on the pathogenesis of DCM.Results: Compared with control group, high glucose markedly increased YAP mRNA and protein expression in DCM and CFs. Inhibition of YAP decreased myocardial fibrosis and improved cardiac function in the DCM model and decreased the expression of CTGF and fibronectin in CFs. The result suggested that YAP plays a key role in the pathological progression of DCM, and the underlying mechanisms may be associated with TEAD and CTGF.Discussion: We found that the expression of YAP was increased both in vivo and in vitro, suggesting that YAP is closely related to DCM, and YAP knockdown can reduce myocardial fibrosis in rat model of DCM by reducing the expression of PAI-1, collagen I, collagen III, CTGF and profilin, as well as the expression of CTGF and fibronectin in CFs. This study revealed that YAP plays an important role in the pathological process of diabetic cardiomyopathy, and down-regulation of YAP expression may provide a new therapeutic target for DCM.Keywords: diabetic cardiomyopathy, yes-associated protein, myocardial fibrosis
format	article
author	Hu M Wang H Li S Yan F Fu C Li L Yu Y Xiong J Dong B
author_facet	Hu M Wang H Li S Yan F Fu C Li L Yu Y Xiong J Dong B
author_sort	Hu M
title	Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy
title_short	Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy
title_full	Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy
title_fullStr	Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy
title_full_unstemmed	Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy
title_sort	yes-associated protein is involved in myocardial fibrosis in rats with diabetic cardiomyopathy
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/4992283811d749e99197a35cea47b5f5
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Yes-Associated Protein is Involved in Myocardial Fibrosis in Rats with Diabetic Cardiomyopathy

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