Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect

Abstract We previously reported the development of an osteogenic bone filler scaffold consisting of degradable polyurethane, hydroxyapatite, and decellularized bovine bone particles. The current study was aimed at evaluating the use of this scaffold as a means of local antibiotic delivery to prevent...

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Autores principales:	Karen E. Beenken, Mara J. Campbell, Aura M. Ramirez, Karrar Alghazali, Christopher M. Walker, Bailey Jackson, Christopher Griffin, William King, Shawn E. Bourdo, Rebecca Rifkin, Silke Hecht, Daniel G. Meeker, David E. Anderson, Alexandru S. Biris, Mark S. Smeltzer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/499612545c514ba09349c6d9e0ce2907
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spelling	oai:doaj.org-article:499612545c514ba09349c6d9e0ce29072021-12-02T16:50:27ZEvaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect10.1038/s41598-021-89830-z2045-2322https://doaj.org/article/499612545c514ba09349c6d9e0ce29072021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89830-zhttps://doaj.org/toc/2045-2322Abstract We previously reported the development of an osteogenic bone filler scaffold consisting of degradable polyurethane, hydroxyapatite, and decellularized bovine bone particles. The current study was aimed at evaluating the use of this scaffold as a means of local antibiotic delivery to prevent infection in a bone defect contaminated with Staphylococcus aureus. We evaluated two scaffold formulations with the same component ratios but differing overall porosity and surface area. Studies with vancomycin, daptomycin, and gentamicin confirmed that antibiotic uptake was concentration dependent and that increased porosity correlated with increased uptake and prolonged antibiotic release. We also demonstrate that vancomycin can be passively loaded into either formulation in sufficient concentration to prevent infection in a rabbit model of a contaminated segmental bone defect. Moreover, even in those few cases in which complete eradication was not achieved, the number of viable bacteria in the bone was significantly reduced by treatment and there was no radiographic evidence of osteomyelitis. Radiographs and microcomputed tomography (µCT) analysis from the in vivo studies also suggested that the addition of vancomycin did not have any significant effect on the scaffold itself. These results demonstrate the potential utility of our bone regeneration scaffold for local antibiotic delivery to prevent infection in contaminated bone defects.Karen E. BeenkenMara J. CampbellAura M. RamirezKarrar AlghazaliChristopher M. WalkerBailey JacksonChristopher GriffinWilliam KingShawn E. BourdoRebecca RifkinSilke HechtDaniel G. MeekerDavid E. AndersonAlexandru S. BirisMark S. SmeltzerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karen E. Beenken Mara J. Campbell Aura M. Ramirez Karrar Alghazali Christopher M. Walker Bailey Jackson Christopher Griffin William King Shawn E. Bourdo Rebecca Rifkin Silke Hecht Daniel G. Meeker David E. Anderson Alexandru S. Biris Mark S. Smeltzer Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect
description	Abstract We previously reported the development of an osteogenic bone filler scaffold consisting of degradable polyurethane, hydroxyapatite, and decellularized bovine bone particles. The current study was aimed at evaluating the use of this scaffold as a means of local antibiotic delivery to prevent infection in a bone defect contaminated with Staphylococcus aureus. We evaluated two scaffold formulations with the same component ratios but differing overall porosity and surface area. Studies with vancomycin, daptomycin, and gentamicin confirmed that antibiotic uptake was concentration dependent and that increased porosity correlated with increased uptake and prolonged antibiotic release. We also demonstrate that vancomycin can be passively loaded into either formulation in sufficient concentration to prevent infection in a rabbit model of a contaminated segmental bone defect. Moreover, even in those few cases in which complete eradication was not achieved, the number of viable bacteria in the bone was significantly reduced by treatment and there was no radiographic evidence of osteomyelitis. Radiographs and microcomputed tomography (µCT) analysis from the in vivo studies also suggested that the addition of vancomycin did not have any significant effect on the scaffold itself. These results demonstrate the potential utility of our bone regeneration scaffold for local antibiotic delivery to prevent infection in contaminated bone defects.
format	article
author	Karen E. Beenken Mara J. Campbell Aura M. Ramirez Karrar Alghazali Christopher M. Walker Bailey Jackson Christopher Griffin William King Shawn E. Bourdo Rebecca Rifkin Silke Hecht Daniel G. Meeker David E. Anderson Alexandru S. Biris Mark S. Smeltzer
author_facet	Karen E. Beenken Mara J. Campbell Aura M. Ramirez Karrar Alghazali Christopher M. Walker Bailey Jackson Christopher Griffin William King Shawn E. Bourdo Rebecca Rifkin Silke Hecht Daniel G. Meeker David E. Anderson Alexandru S. Biris Mark S. Smeltzer
author_sort	Karen E. Beenken
title	Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect
title_short	Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect
title_full	Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect
title_fullStr	Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect
title_full_unstemmed	Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect
title_sort	evaluation of a bone filler scaffold for local antibiotic delivery to prevent staphylococcus aureus infection in a contaminated bone defect
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/499612545c514ba09349c6d9e0ce2907
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Evaluation of a bone filler scaffold for local antibiotic delivery to prevent Staphylococcus aureus infection in a contaminated bone defect

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