Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I

Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)—the gold-standard biomarker of myocardial infarction (MI) (or “heart attack”)—becomes crucial in the early diagnosis a...

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Autores principales:	Remya Radha, Mohammad Hussein Al-Sayah
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	myocardial infarction cardiac troponin cTnI-detection calcein liposomes fluorescence signal quantification Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/499663c0eb4844afb9a147a6b59db920
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id	oai:doaj.org-article:499663c0eb4844afb9a147a6b59db920
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spelling	oai:doaj.org-article:499663c0eb4844afb9a147a6b59db9202021-11-25T18:28:57ZDevelopment of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I10.3390/molecules262269881420-3049https://doaj.org/article/499663c0eb4844afb9a147a6b59db9202021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6988https://doaj.org/toc/1420-3049Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)—the gold-standard biomarker of myocardial infarction (MI) (or “heart attack”)—becomes crucial in the early diagnosis and treatment of myocardial infarction (MI). This study investigates the development of an efficient fluorescent “sandwich” immunoassay using liposome-based fluorescent signal amplification and thereby enables the sensing and quantification of serum-cTnI at a concentration relevant to clinical settings. The calcein-loaded liposomes were utilized as fluorescent nano vehicles, and these have exhibited appropriate stability and efficient fluorescent properties. The standardized assay was sensitive and selective towards cTnI in both physiological buffer solutions and spiked human serum samples. The novel assay presented noble analytical results with sound dynamic linearity over a wide concentration range of 0 to 320 ng/mL and a detection limit of 6.5 ng/mL for cTnI in the spiked human serum.Remya RadhaMohammad Hussein Al-SayahMDPI AGarticlemyocardial infarctioncardiac troponincTnI-detectioncalcein liposomesfluorescencesignal quantificationOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6988, p 6988 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	myocardial infarction cardiac troponin cTnI-detection calcein liposomes fluorescence signal quantification Organic chemistry QD241-441
spellingShingle	myocardial infarction cardiac troponin cTnI-detection calcein liposomes fluorescence signal quantification Organic chemistry QD241-441 Remya Radha Mohammad Hussein Al-Sayah Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
description	Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)—the gold-standard biomarker of myocardial infarction (MI) (or “heart attack”)—becomes crucial in the early diagnosis and treatment of myocardial infarction (MI). This study investigates the development of an efficient fluorescent “sandwich” immunoassay using liposome-based fluorescent signal amplification and thereby enables the sensing and quantification of serum-cTnI at a concentration relevant to clinical settings. The calcein-loaded liposomes were utilized as fluorescent nano vehicles, and these have exhibited appropriate stability and efficient fluorescent properties. The standardized assay was sensitive and selective towards cTnI in both physiological buffer solutions and spiked human serum samples. The novel assay presented noble analytical results with sound dynamic linearity over a wide concentration range of 0 to 320 ng/mL and a detection limit of 6.5 ng/mL for cTnI in the spiked human serum.
format	article
author	Remya Radha Mohammad Hussein Al-Sayah
author_facet	Remya Radha Mohammad Hussein Al-Sayah
author_sort	Remya Radha
title	Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
title_short	Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
title_full	Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
title_fullStr	Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
title_full_unstemmed	Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I
title_sort	development of liposome-based immunoassay for the detection of cardiac troponin i
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/499663c0eb4844afb9a147a6b59db920
work_keys_str_mv	AT remyaradha developmentofliposomebasedimmunoassayforthedetectionofcardiactroponini AT mohammadhusseinalsayah developmentofliposomebasedimmunoassayforthedetectionofcardiactroponini
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Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I

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