Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene

Worranan Rangsimawong, Praneet Opanasopit, Theerasak Rojanarata, Tanasait Ngawhirunpat Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand Abstract: The effect of low frequency sonophoresis (SN, 20 kHz) on the skin transport of sodium fluorescein (NaFI)-loaded liposomes was investig...

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Autores principales:	Rangsimawong W, Opanasopit P, Rojanarata T, Ngawhirunpat T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	sonophoresis PEGylated liposomes hydrophilic compound follicular pathway Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/499b9af089dc49beb0c758449004144c
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spelling	oai:doaj.org-article:499b9af089dc49beb0c758449004144c2021-12-02T02:28:14ZMechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene1178-2013https://doaj.org/article/499b9af089dc49beb0c758449004144c2015-12-01T00:00:00Zhttps://www.dovepress.com/mechanistic-study-of-decreased-skin-penetration-using-a-combination-of-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Worranan Rangsimawong, Praneet Opanasopit, Theerasak Rojanarata, Tanasait Ngawhirunpat Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand Abstract: The effect of low frequency sonophoresis (SN, 20 kHz) on the skin transport of sodium fluorescein (NaFI)-loaded liposomes was investigated. An in vitro skin penetration study in open and blocked hair follicles was performed, and confocal laser scanning microscopy and scanning electron microscopy were used to visualize the penetration pathways. The results showed that SN significantly increased the flux of NaFI solution, whereas it significantly decreased the flux of NaFI-loaded polyethylene glycol-coated (PEGylated) liposomes with D-limonene (PL-LI). SN did not significantly affect the flux of NaFI-loaded conventional liposomes and PEGylated liposomes. In the blocked follicles, the flux of NaFI-loaded PL-LI both with and without SN decreased, indicating that NaFI-loaded PL-LI penetrated the skin via the transfollicular pathway. A confocal laser scanning microscopy image showed that in the skin without SN, the fluorescence intensity of NaFI-loaded PL-LI was observed in the skin and along the length of hair inside the skin, whereas in the skin with applied SN, the fluorescence intensity was detected only on the top of hair outside the skin. From scanning electron microscopy images, SN dislocated the corneocytes and reduced the deposition of PL-LI around hair follicles. These results revealed that SN may partially plug hair follicle orifices and reduce percutaneous absorption through the follicular pathway. Keywords: sonophoresis, PEGylated liposomes, hydrophilic compound, follicular pathwayRangsimawong WOpanasopit PRojanarata TNgawhirunpat TDove Medical PressarticlesonophoresisPEGylated liposomeshydrophilic compoundfollicular pathwayMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7413-7423 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	sonophoresis PEGylated liposomes hydrophilic compound follicular pathway Medicine (General) R5-920
spellingShingle	sonophoresis PEGylated liposomes hydrophilic compound follicular pathway Medicine (General) R5-920 Rangsimawong W Opanasopit P Rojanarata T Ngawhirunpat T Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene
description	Worranan Rangsimawong, Praneet Opanasopit, Theerasak Rojanarata, Tanasait Ngawhirunpat Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand Abstract: The effect of low frequency sonophoresis (SN, 20 kHz) on the skin transport of sodium fluorescein (NaFI)-loaded liposomes was investigated. An in vitro skin penetration study in open and blocked hair follicles was performed, and confocal laser scanning microscopy and scanning electron microscopy were used to visualize the penetration pathways. The results showed that SN significantly increased the flux of NaFI solution, whereas it significantly decreased the flux of NaFI-loaded polyethylene glycol-coated (PEGylated) liposomes with D-limonene (PL-LI). SN did not significantly affect the flux of NaFI-loaded conventional liposomes and PEGylated liposomes. In the blocked follicles, the flux of NaFI-loaded PL-LI both with and without SN decreased, indicating that NaFI-loaded PL-LI penetrated the skin via the transfollicular pathway. A confocal laser scanning microscopy image showed that in the skin without SN, the fluorescence intensity of NaFI-loaded PL-LI was observed in the skin and along the length of hair inside the skin, whereas in the skin with applied SN, the fluorescence intensity was detected only on the top of hair outside the skin. From scanning electron microscopy images, SN dislocated the corneocytes and reduced the deposition of PL-LI around hair follicles. These results revealed that SN may partially plug hair follicle orifices and reduce percutaneous absorption through the follicular pathway. Keywords: sonophoresis, PEGylated liposomes, hydrophilic compound, follicular pathway
format	article
author	Rangsimawong W Opanasopit P Rojanarata T Ngawhirunpat T
author_facet	Rangsimawong W Opanasopit P Rojanarata T Ngawhirunpat T
author_sort	Rangsimawong W
title	Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene
title_short	Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene
title_full	Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene
title_fullStr	Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene
title_full_unstemmed	Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene
title_sort	mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded pegylated liposomes with d-limonene
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/499b9af089dc49beb0c758449004144c
work_keys_str_mv	AT rangsimawongw mechanisticstudyofdecreasedskinpenetrationusingacombinationofsonophoresiswithsodiumfluoresceinloadedpegylatedliposomeswithdlimonene AT opanasopitp mechanisticstudyofdecreasedskinpenetrationusingacombinationofsonophoresiswithsodiumfluoresceinloadedpegylatedliposomeswithdlimonene AT rojanaratat mechanisticstudyofdecreasedskinpenetrationusingacombinationofsonophoresiswithsodiumfluoresceinloadedpegylatedliposomeswithdlimonene AT ngawhirunpatt mechanisticstudyofdecreasedskinpenetrationusingacombinationofsonophoresiswithsodiumfluoresceinloadedpegylatedliposomeswithdlimonene
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Mechanistic study of decreased skin penetration using a combination of sonophoresis with sodium fluorescein-loaded PEGylated liposomes with D-limonene

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