Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

Abstract Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we inv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun-Kyu Kim, Young-Jin Youn, Yu-Bin Lee, Sun-Hwa Kim, Dong-Keun Song, Minsang Shin, Hee Kyung Jin, Jae-sung Bae, Sanjeeb Shrestha, Chang-Won Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49ad586bf1c545759c45dcf051fef806
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:49ad586bf1c545759c45dcf051fef806
record_format	dspace
spelling	oai:doaj.org-article:49ad586bf1c545759c45dcf051fef8062021-12-02T14:26:16ZExtracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics10.1038/s41598-021-87891-82045-2322https://doaj.org/article/49ad586bf1c545759c45dcf051fef8062021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87891-8https://doaj.org/toc/2045-2322Abstract Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.Jun-Kyu KimYoung-Jin YounYu-Bin LeeSun-Hwa KimDong-Keun SongMinsang ShinHee Kyung JinJae-sung BaeSanjeeb ShresthaChang-Won HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun-Kyu Kim Young-Jin Youn Yu-Bin Lee Sun-Hwa Kim Dong-Keun Song Minsang Shin Hee Kyung Jin Jae-sung Bae Sanjeeb Shrestha Chang-Won Hong Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics
description	Abstract Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.
format	article
author	Jun-Kyu Kim Young-Jin Youn Yu-Bin Lee Sun-Hwa Kim Dong-Keun Song Minsang Shin Hee Kyung Jin Jae-sung Bae Sanjeeb Shrestha Chang-Won Hong
author_facet	Jun-Kyu Kim Young-Jin Youn Yu-Bin Lee Sun-Hwa Kim Dong-Keun Song Minsang Shin Hee Kyung Jin Jae-sung Bae Sanjeeb Shrestha Chang-Won Hong
author_sort	Jun-Kyu Kim
title	Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics
title_short	Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics
title_full	Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics
title_fullStr	Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics
title_full_unstemmed	Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics
title_sort	extracellular vesicles from dhl-60 cells as delivery vehicles for diverse therapeutics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/49ad586bf1c545759c45dcf051fef806
work_keys_str_mv	AT junkyukim extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT youngjinyoun extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT yubinlee extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT sunhwakim extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT dongkeunsong extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT minsangshin extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT heekyungjin extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT jaesungbae extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT sanjeebshrestha extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics AT changwonhong extracellularvesiclesfromdhl60cellsasdeliveryvehiclesfordiversetherapeutics
_version_	1718391365509840896

Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

Ejemplares similares