Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)

During the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide...

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Autores principales:	Ketut Ratnayani, I Ketut Suter, Nyoman Semadi Antara, I Nengah Kencana Putra
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2019
Materias:	pigeon pea peptide fraction ACE inhibitory germination Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/49ae70dc05a94430a6e69095701b7d0e
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spelling	oai:doaj.org-article:49ae70dc05a94430a6e69095701b7d0e2021-12-02T10:51:30ZAngiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)1411-94202460-157810.22146/ijc.37513https://doaj.org/article/49ae70dc05a94430a6e69095701b7d0e2019-08-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/37513https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578During the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide extract at various germination times and to carry out the fractionation to the extract to get the most active peptide fraction. The results showed that the highest activity of peptide extract was found on the 4th-day germination of pigeon pea with an IC50 value of 63.46 μg/mL. The peptide extract was further fractionated by centrifugal ultrafiltration method and it was found that the peptide fraction < 3 kDa had the highest ACE inhibitory activity with an IC50 value of 57.79 μg/mL. The result of identification with the LCMS method to the fraction was able to detect 4 types of the peptide with a molecular weight of 230.304, 294.303, 441.436, and 570.591 Da. These results suggested that the peptide fraction of germinated pigeon pea has the potency as an ACE inhibitory nutraceutical.Ketut RatnayaniI Ketut SuterNyoman Semadi AntaraI Nengah Kencana PutraDepartment of Chemistry, Universitas Gadjah Madaarticlepigeon peapeptide fractionACE inhibitorygerminationChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 19, Iss 4, Pp 900-906 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	pigeon pea peptide fraction ACE inhibitory germination Chemistry QD1-999
spellingShingle	pigeon pea peptide fraction ACE inhibitory germination Chemistry QD1-999 Ketut Ratnayani I Ketut Suter Nyoman Semadi Antara I Nengah Kencana Putra Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
description	During the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide extract at various germination times and to carry out the fractionation to the extract to get the most active peptide fraction. The results showed that the highest activity of peptide extract was found on the 4th-day germination of pigeon pea with an IC50 value of 63.46 μg/mL. The peptide extract was further fractionated by centrifugal ultrafiltration method and it was found that the peptide fraction < 3 kDa had the highest ACE inhibitory activity with an IC50 value of 57.79 μg/mL. The result of identification with the LCMS method to the fraction was able to detect 4 types of the peptide with a molecular weight of 230.304, 294.303, 441.436, and 570.591 Da. These results suggested that the peptide fraction of germinated pigeon pea has the potency as an ACE inhibitory nutraceutical.
format	article
author	Ketut Ratnayani I Ketut Suter Nyoman Semadi Antara I Nengah Kencana Putra
author_facet	Ketut Ratnayani I Ketut Suter Nyoman Semadi Antara I Nengah Kencana Putra
author_sort	Ketut Ratnayani
title	Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
title_short	Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
title_full	Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
title_fullStr	Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
title_full_unstemmed	Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
title_sort	angiotensin converting enzyme (ace) inhibitory activity of peptide fraction of germinated pigeon pea (<i>cajanus cajan</i> (l.) millsp.)
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2019
url	https://doaj.org/article/49ae70dc05a94430a6e69095701b7d0e
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Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)

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