Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix

Research on the bioactive components of herbal medicines have been conducted mainly on the secondary metabolites of herbal plants. Accordingly, limited information is available on primary metabolites (carbohydrates, amino acids, lipids, and nucleic acids) and their biological effects. Here, we focus...

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Autores principales:	Akiko Inujima, Keiichi Koizumi, Naotoshi Shibahara
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Glycyrrhizae radix Nuclear Factor-κB (NF-κB) RNA RNA-Sequencing Tumor Necrosis Factor-α (TNF-α) Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/49afb2e189524770a44dc16e93c342df
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spelling	oai:doaj.org-article:49afb2e189524770a44dc16e93c342df2021-12-04T04:33:05ZImmunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix0753-332210.1016/j.biopha.2021.112457https://doaj.org/article/49afb2e189524770a44dc16e93c342df2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012439https://doaj.org/toc/0753-3322Research on the bioactive components of herbal medicines have been conducted mainly on the secondary metabolites of herbal plants. Accordingly, limited information is available on primary metabolites (carbohydrates, amino acids, lipids, and nucleic acids) and their biological effects. Here, we focused on the heat-resistant RNA of a decoction of Glycyrrhizae Radix and showed its immunostimulatory effects. The RNA activated NF-κB/AP-1 and induced TNF-α production in murine macrophages. Further analysis revealed that the RNA was around 90 nucleotides long. RNA sequencing (RNA-Seq) by next generation sequencing (NGS) showed that approximately 30% of the NGS reads were mapped to the genome of Glycyrrhiza uralensis, which is plant material of Glycyrrhizae Radix. Further analysis of the other 70% of reads indicated that the RNA contained RNA sequences that could be mapped to various microorganisms. Together, these results propose nucleic acids as a new research field in the bioactive components of herbal medicines.Akiko InujimaKeiichi KoizumiNaotoshi ShibaharaElsevierarticleGlycyrrhizae radixNuclear Factor-κB (NF-κB)RNARNA-SequencingTumor Necrosis Factor-α (TNF-α)Therapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112457- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Glycyrrhizae radix Nuclear Factor-κB (NF-κB) RNA RNA-Sequencing Tumor Necrosis Factor-α (TNF-α) Therapeutics. Pharmacology RM1-950
spellingShingle	Glycyrrhizae radix Nuclear Factor-κB (NF-κB) RNA RNA-Sequencing Tumor Necrosis Factor-α (TNF-α) Therapeutics. Pharmacology RM1-950 Akiko Inujima Keiichi Koizumi Naotoshi Shibahara Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix
description	Research on the bioactive components of herbal medicines have been conducted mainly on the secondary metabolites of herbal plants. Accordingly, limited information is available on primary metabolites (carbohydrates, amino acids, lipids, and nucleic acids) and their biological effects. Here, we focused on the heat-resistant RNA of a decoction of Glycyrrhizae Radix and showed its immunostimulatory effects. The RNA activated NF-κB/AP-1 and induced TNF-α production in murine macrophages. Further analysis revealed that the RNA was around 90 nucleotides long. RNA sequencing (RNA-Seq) by next generation sequencing (NGS) showed that approximately 30% of the NGS reads were mapped to the genome of Glycyrrhiza uralensis, which is plant material of Glycyrrhizae Radix. Further analysis of the other 70% of reads indicated that the RNA contained RNA sequences that could be mapped to various microorganisms. Together, these results propose nucleic acids as a new research field in the bioactive components of herbal medicines.
format	article
author	Akiko Inujima Keiichi Koizumi Naotoshi Shibahara
author_facet	Akiko Inujima Keiichi Koizumi Naotoshi Shibahara
author_sort	Akiko Inujima
title	Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix
title_short	Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix
title_full	Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix
title_fullStr	Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix
title_full_unstemmed	Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix
title_sort	immunostimulatory properties of heat-resistant rna in a decoction of glycyrrhizae radix
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/49afb2e189524770a44dc16e93c342df
work_keys_str_mv	AT akikoinujima immunostimulatorypropertiesofheatresistantrnainadecoctionofglycyrrhizaeradix AT keiichikoizumi immunostimulatorypropertiesofheatresistantrnainadecoctionofglycyrrhizaeradix AT naotoshishibahara immunostimulatorypropertiesofheatresistantrnainadecoctionofglycyrrhizaeradix
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Immunostimulatory properties of heat-resistant RNA in a decoction of Glycyrrhizae Radix

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