Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro

Jamal Almowalad,1 Sukrut Somani,1 Partha Laskar,1 Jitkasem Meewan,1 Rothwelle J Tate,1 Margaret Mullin,2 Christine Dufès1 1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, UK; 2College of Medical, Veterinary and Life Sciences, University of Glasg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Almowalad J, Somani S, Laskar P, Meewan J, Tate RJ, Mullin M, Dufès C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	gold nanocages cancer targeting transfection efficacy lactoferrin polethylenimine polyethylene glycol Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/49bc30e1a58048e891609b3f209e2dac
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:49bc30e1a58048e891609b3f209e2dac
record_format	dspace
spelling	oai:doaj.org-article:49bc30e1a58048e891609b3f209e2dac2021-12-02T18:47:42ZLactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro1178-2013https://doaj.org/article/49bc30e1a58048e891609b3f209e2dac2021-06-01T00:00:00Zhttps://www.dovepress.com/lactoferrin-bearing-gold-nanocages-for-gene-delivery-in-prostate-cance-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Jamal Almowalad,1 Sukrut Somani,1 Partha Laskar,1 Jitkasem Meewan,1 Rothwelle J Tate,1 Margaret Mullin,2 Christine Dufès1 1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, UK; 2College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UKCorrespondence: Christine DufèsStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, UKTel +44 1415483796Fax +44 1415522562Email C.Dufes@strath.ac.ukBackground: Gold nanocages have been widely used as multifunctional platforms for drug and gene delivery, as well as photothermal agents for cancer therapy. However, their potential as gene delivery systems for cancer treatment has been reported in combination with chemotherapeutics and photothermal therapy, but not in isolation so far. The purpose of this work was to investigate whether the conjugation of gold nanocages with the cancer targeting ligand lactoferrin, polyethylene glycol and polyethylenimine could lead to enhanced transfection efficiency on prostate cancer cells in vitro, without assistance of external stimulation.Methods: Novel lactoferrin-bearing gold nanocages conjugated to polyethylenimine and polyethylene glycol have been synthesized and characterized. Their transfection efficacy and cytotoxicity were assessed on PC-3 prostate cancer cell line following complexation with a plasmid DNA.Results: Lactoferrin-bearing gold nanocages, alone or conjugated with polyethylenimine and polyethylene glycol, were able to condense DNA at conjugate:DNA weight ratios 5:1 and higher. Among all gold conjugates, the highest gene expression was obtained following treatment with gold complex conjugated with polyethylenimine and lactoferrin, at weight ratio 40:1, which was 1.71-fold higher than with polyethylenimine. This might be due to the increased DNA cellular uptake observed with this conjugate, by up to 8.65-fold in comparison with naked DNA.Conclusion: Lactoferrin-bearing gold nanocages conjugates are highly promising gene delivery systems to prostate cancer cells.Keywords: gold nanocages, cancer targeting, transfection efficacy, lactoferrin, polethylenimine, polyethylene glycolAlmowalad JSomani SLaskar PMeewan JTate RJMullin MDufès CDove Medical Pressarticlegold nanocagescancer targetingtransfection efficacylactoferrinpolethyleniminepolyethylene glycolMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 4391-4407 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanocages cancer targeting transfection efficacy lactoferrin polethylenimine polyethylene glycol Medicine (General) R5-920
spellingShingle	gold nanocages cancer targeting transfection efficacy lactoferrin polethylenimine polyethylene glycol Medicine (General) R5-920 Almowalad J Somani S Laskar P Meewan J Tate RJ Mullin M Dufès C Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro
description	Jamal Almowalad,1 Sukrut Somani,1 Partha Laskar,1 Jitkasem Meewan,1 Rothwelle J Tate,1 Margaret Mullin,2 Christine Dufès1 1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, UK; 2College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UKCorrespondence: Christine DufèsStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, UKTel +44 1415483796Fax +44 1415522562Email C.Dufes@strath.ac.ukBackground: Gold nanocages have been widely used as multifunctional platforms for drug and gene delivery, as well as photothermal agents for cancer therapy. However, their potential as gene delivery systems for cancer treatment has been reported in combination with chemotherapeutics and photothermal therapy, but not in isolation so far. The purpose of this work was to investigate whether the conjugation of gold nanocages with the cancer targeting ligand lactoferrin, polyethylene glycol and polyethylenimine could lead to enhanced transfection efficiency on prostate cancer cells in vitro, without assistance of external stimulation.Methods: Novel lactoferrin-bearing gold nanocages conjugated to polyethylenimine and polyethylene glycol have been synthesized and characterized. Their transfection efficacy and cytotoxicity were assessed on PC-3 prostate cancer cell line following complexation with a plasmid DNA.Results: Lactoferrin-bearing gold nanocages, alone or conjugated with polyethylenimine and polyethylene glycol, were able to condense DNA at conjugate:DNA weight ratios 5:1 and higher. Among all gold conjugates, the highest gene expression was obtained following treatment with gold complex conjugated with polyethylenimine and lactoferrin, at weight ratio 40:1, which was 1.71-fold higher than with polyethylenimine. This might be due to the increased DNA cellular uptake observed with this conjugate, by up to 8.65-fold in comparison with naked DNA.Conclusion: Lactoferrin-bearing gold nanocages conjugates are highly promising gene delivery systems to prostate cancer cells.Keywords: gold nanocages, cancer targeting, transfection efficacy, lactoferrin, polethylenimine, polyethylene glycol
format	article
author	Almowalad J Somani S Laskar P Meewan J Tate RJ Mullin M Dufès C
author_facet	Almowalad J Somani S Laskar P Meewan J Tate RJ Mullin M Dufès C
author_sort	Almowalad J
title	Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro
title_short	Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro
title_full	Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro
title_fullStr	Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro
title_full_unstemmed	Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro
title_sort	lactoferrin-bearing gold nanocages for gene delivery in prostate cancer cells in vitro
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/49bc30e1a58048e891609b3f209e2dac
work_keys_str_mv	AT almowaladj lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro AT somanis lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro AT laskarp lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro AT meewanj lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro AT taterj lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro AT mullinm lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro AT dufesc lactoferrinbearinggoldnanocagesforgenedeliveryinprostatecancercellsinvitro
_version_	1718377619491127296

Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro

Ejemplares similares