GATA3 regulates FLG and FLG2 expression in human primary keratinocytes

Abstract GATA3 is a transcription factor with an important role in atopic diseases because of its role in the differentiation of Th2 lymphocytes. Moreover, GATA3 is expressed in keratinocytes and has a role in keratinocyte differentiation and the establishment of the epidermal barrier. In this study...

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Autores principales:	Jana Zeitvogel, Neele Jokmin, Samira Rieker, Ilona Klug, Christina Brandenberger, Thomas Werfel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49c20770e63c4aab8d6d53bb6e0dd39c
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spelling	oai:doaj.org-article:49c20770e63c4aab8d6d53bb6e0dd39c2021-12-02T11:41:23ZGATA3 regulates FLG and FLG2 expression in human primary keratinocytes10.1038/s41598-017-10252-x2045-2322https://doaj.org/article/49c20770e63c4aab8d6d53bb6e0dd39c2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10252-xhttps://doaj.org/toc/2045-2322Abstract GATA3 is a transcription factor with an important role in atopic diseases because of its role in the differentiation of Th2 lymphocytes. Moreover, GATA3 is expressed in keratinocytes and has a role in keratinocyte differentiation and the establishment of the epidermal barrier. In this study, we investigated the role of GATA3 in keratinocytes in the context of epidermal barrier integrity under inflammatory skin conditions. When analysing skin samples from atopic dermatitis and psoriasis patients or healthy controls, we detected decreased expression of GATA3 in the stratum spinosum and stratum granulosum of atopic dermatitis and psoriasis patients when compared to healthy controls. Our cell cultures experiments revealed that a downregulation in GATA3 by shRNA leads to a significant reduction of filaggrin mRNA under atopic dermatitis-like conditions in keratinocytes. Overexpression of GATA3 in keratinocytes reversed this effect and significantly upregulated filaggrin and, furthermore, filaggrin-2 mRNA expression. Our results demonstrate that GATA3 is involved in the regulation of filaggrin and filaggrin-2 expression during inflammatory conditions in the skin. Thus, GATA3 may be of special importance for the establishment and maintenance of an intact epidermal barrier, especially in atopic dermatitis.Jana ZeitvogelNeele JokminSamira RiekerIlona KlugChristina BrandenbergerThomas WerfelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jana Zeitvogel Neele Jokmin Samira Rieker Ilona Klug Christina Brandenberger Thomas Werfel GATA3 regulates FLG and FLG2 expression in human primary keratinocytes
description	Abstract GATA3 is a transcription factor with an important role in atopic diseases because of its role in the differentiation of Th2 lymphocytes. Moreover, GATA3 is expressed in keratinocytes and has a role in keratinocyte differentiation and the establishment of the epidermal barrier. In this study, we investigated the role of GATA3 in keratinocytes in the context of epidermal barrier integrity under inflammatory skin conditions. When analysing skin samples from atopic dermatitis and psoriasis patients or healthy controls, we detected decreased expression of GATA3 in the stratum spinosum and stratum granulosum of atopic dermatitis and psoriasis patients when compared to healthy controls. Our cell cultures experiments revealed that a downregulation in GATA3 by shRNA leads to a significant reduction of filaggrin mRNA under atopic dermatitis-like conditions in keratinocytes. Overexpression of GATA3 in keratinocytes reversed this effect and significantly upregulated filaggrin and, furthermore, filaggrin-2 mRNA expression. Our results demonstrate that GATA3 is involved in the regulation of filaggrin and filaggrin-2 expression during inflammatory conditions in the skin. Thus, GATA3 may be of special importance for the establishment and maintenance of an intact epidermal barrier, especially in atopic dermatitis.
format	article
author	Jana Zeitvogel Neele Jokmin Samira Rieker Ilona Klug Christina Brandenberger Thomas Werfel
author_facet	Jana Zeitvogel Neele Jokmin Samira Rieker Ilona Klug Christina Brandenberger Thomas Werfel
author_sort	Jana Zeitvogel
title	GATA3 regulates FLG and FLG2 expression in human primary keratinocytes
title_short	GATA3 regulates FLG and FLG2 expression in human primary keratinocytes
title_full	GATA3 regulates FLG and FLG2 expression in human primary keratinocytes
title_fullStr	GATA3 regulates FLG and FLG2 expression in human primary keratinocytes
title_full_unstemmed	GATA3 regulates FLG and FLG2 expression in human primary keratinocytes
title_sort	gata3 regulates flg and flg2 expression in human primary keratinocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/49c20770e63c4aab8d6d53bb6e0dd39c
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_version_	1718395396003201024

GATA3 regulates FLG and FLG2 expression in human primary keratinocytes

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