Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)

Abstract. Hastuti SD, Barton MD, Pyecroft SB, Costabile M. 2020. Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer). Biodiversitas 21: 3034-3040. Complement proteins are one component of innate immunity present in fish. The measurement of c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sri Dwi Hastuti, MARY D. BARTON, STEPHEN B. PYECROFT, MAURIZIO COSTABILE
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	alternate complement pathway, acp, asian seabass, assay optimization Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/49c9cb8d1a0b4bf19c41baa6b48f9653
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:49c9cb8d1a0b4bf19c41baa6b48f9653
record_format	dspace
spelling	oai:doaj.org-article:49c9cb8d1a0b4bf19c41baa6b48f96532021-11-22T00:40:52ZAssay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)1412-033X2085-472210.13057/biodiv/d210721https://doaj.org/article/49c9cb8d1a0b4bf19c41baa6b48f96532020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5458https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Hastuti SD, Barton MD, Pyecroft SB, Costabile M. 2020. Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer). Biodiversitas 21: 3034-3040. Complement proteins are one component of innate immunity present in fish. The measurement of complement activity in fish can be used to monitor the health status of fish. This is particularly important in Asian seabass (Lates calcarifer) aquaculture, where disease can impact on productivity. We have found an optimal condition assay for measuring the alternate complement pathway (ACP) activity of Asian seabass which includes Magnesium chloride (MgCl2) concentration, buffer pH, incubation temperature and incubation time. The assay was optimized using pooled serum of Asian seabass, diluted in Magnesium ethylenediamine tetraacetic acid gelatine veronal buffer (Mg-EDTA-GVB) and added with Rabbit red blood cells (RRBC) suspension. Subsequently, the suspension was incubated and centrifuged. The supernatant was removed and transferred to a well plate and the optical density (OD) was measured at 540 nm. The optimal condition obtained included a 7.5 mM MgCl2, pH optimum of 7.5, 25°C incubation temperature, and a 30 minutes incubation period. The presently developed assay was robust, rapid, and reliable to be used in monitoring the health status of Asian seabass in aquaculture farms. It can be used as guidance in further immunological studies on this fish.Sri Dwi HastutiMARY D. BARTONSTEPHEN B. PYECROFTMAURIZIO COSTABILEMBI & UNS Soloarticlealternate complement pathway, acp, asian seabass, assay optimizationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	alternate complement pathway, acp, asian seabass, assay optimization Biology (General) QH301-705.5
spellingShingle	alternate complement pathway, acp, asian seabass, assay optimization Biology (General) QH301-705.5 Sri Dwi Hastuti MARY D. BARTON STEPHEN B. PYECROFT MAURIZIO COSTABILE Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
description	Abstract. Hastuti SD, Barton MD, Pyecroft SB, Costabile M. 2020. Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer). Biodiversitas 21: 3034-3040. Complement proteins are one component of innate immunity present in fish. The measurement of complement activity in fish can be used to monitor the health status of fish. This is particularly important in Asian seabass (Lates calcarifer) aquaculture, where disease can impact on productivity. We have found an optimal condition assay for measuring the alternate complement pathway (ACP) activity of Asian seabass which includes Magnesium chloride (MgCl2) concentration, buffer pH, incubation temperature and incubation time. The assay was optimized using pooled serum of Asian seabass, diluted in Magnesium ethylenediamine tetraacetic acid gelatine veronal buffer (Mg-EDTA-GVB) and added with Rabbit red blood cells (RRBC) suspension. Subsequently, the suspension was incubated and centrifuged. The supernatant was removed and transferred to a well plate and the optical density (OD) was measured at 540 nm. The optimal condition obtained included a 7.5 mM MgCl2, pH optimum of 7.5, 25°C incubation temperature, and a 30 minutes incubation period. The presently developed assay was robust, rapid, and reliable to be used in monitoring the health status of Asian seabass in aquaculture farms. It can be used as guidance in further immunological studies on this fish.
format	article
author	Sri Dwi Hastuti MARY D. BARTON STEPHEN B. PYECROFT MAURIZIO COSTABILE
author_facet	Sri Dwi Hastuti MARY D. BARTON STEPHEN B. PYECROFT MAURIZIO COSTABILE
author_sort	Sri Dwi Hastuti
title	Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
title_short	Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
title_full	Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
title_fullStr	Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
title_full_unstemmed	Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
title_sort	assay optimization for measuring the alternate complement pathway activity in asian seabass (lates calcarifer)
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/49c9cb8d1a0b4bf19c41baa6b48f9653
work_keys_str_mv	AT sridwihastuti assayoptimizationformeasuringthealternatecomplementpathwayactivityinasianseabasslatescalcarifer AT marydbarton assayoptimizationformeasuringthealternatecomplementpathwayactivityinasianseabasslatescalcarifer AT stephenbpyecroft assayoptimizationformeasuringthealternatecomplementpathwayactivityinasianseabasslatescalcarifer AT mauriziocostabile assayoptimizationformeasuringthealternatecomplementpathwayactivityinasianseabasslatescalcarifer
_version_	1718418521008898048

Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)

Ejemplares similares