Dysregulation of lncRNAs in autoimmune neuropathies

Abstract Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) and Guillain-Barré syndrome (GBS) are inflammatory neuropathies with different clinical courses but similar underlying mechanisms. Long non-coding RNAs (lncRNAs) might affect pathogenesis of these conditions. In the current pr...

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Autores principales:	Mahdi Gholipour, Mohammad Taheri, Jafar Mehvari Habibabadi, Naghme Nazer, Arezou Sayad, Soudeh Ghafouri-Fard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49cf2d6a751149ecaab4353a9259880a
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spelling	oai:doaj.org-article:49cf2d6a751149ecaab4353a9259880a2021-12-02T18:50:59ZDysregulation of lncRNAs in autoimmune neuropathies10.1038/s41598-021-95466-w2045-2322https://doaj.org/article/49cf2d6a751149ecaab4353a9259880a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95466-whttps://doaj.org/toc/2045-2322Abstract Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) and Guillain-Barré syndrome (GBS) are inflammatory neuropathies with different clinical courses but similar underlying mechanisms. Long non-coding RNAs (lncRNAs) might affect pathogenesis of these conditions. In the current project, we have selected HULC, PVT1, MEG3, SPRY4-IT1, LINC-ROR and DSCAM-AS1 lncRNAs to appraise their transcript levels in the circulation of CIDP and GBS cases versus controls. Expression of HULC was higher in CIDP patients compared with healthy persons (Ratio of mean expression (RME) = 7.62, SE = 0.72, P < 0.001). While expression of this lncRNA was not different between female CIDP cases and female controls, its expression was higher in male CIDP cases compared with male controls (RME = 13.50, SE = 0.98, P < 0.001). Similarly, expression of HULC was higher in total GBS cases compared with healthy persons (RME = 4.57, SE = 0.65, P < 0.001) and in male cases compared with male controls (RME = 5.48, SE = 0.82, P < 0.001). Similar pattern of expression was detected between total cases and total controls. PVT1 was up-regulated in CIDP cases compared with controls (RME = 3.04, SE = 0.51, P < 0.001) and in both male and female CIDP cases compared with sex-matched controls. Similarly, PVT1 was up-regulated in GBS cases compared with controls (RME = 2.99, SE = 0.55, P vale < 0.001) and in total patients compared with total controls (RME = 3.02, SE = 0.43, P < 0.001). Expression levels of DSCAM-AS1 and SPRY4-IT1 were higher in CIDP and GBS cases compared with healthy subjects and in both sexes compared with gender-matched healthy persons. Although LINC-ROR was up-regulated in total CIDP and total GBS cases compared with controls, in sex-based comparisons, it was only up-regulated in male CIDP cases compared with male controls (RME = 3.06, P = 0.03). Finally, expression of MEG3 was up-regulated in all subgroups of patients versus controls except for male GBS controls. SPRY4-IT could differentiate CIDP cases from controls with AUC = 0.84, sensitivity = 0.63 and specificity = 0.97. AUC values of DSCAM-AS1, MEG3, HULC, PVT1 and LINC-ROR were 0.80, 0.75, 0.74, 0.73 and 0.72, respectively. In differentiation between GBS cases and controls, SPRY4-IT and DSCAM-AS1 has the AUC value of 0.8. None of lncRNAs could appropriately differentiate between CIDP and GBS cases. Combination of all lncRNAs could not significantly enhance the diagnostic power. Taken together, these lncRNAs might be involved in the development of CIDP or GBS.Mahdi GholipourMohammad TaheriJafar Mehvari HabibabadiNaghme NazerArezou SayadSoudeh Ghafouri-FardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mahdi Gholipour Mohammad Taheri Jafar Mehvari Habibabadi Naghme Nazer Arezou Sayad Soudeh Ghafouri-Fard Dysregulation of lncRNAs in autoimmune neuropathies
description	Abstract Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) and Guillain-Barré syndrome (GBS) are inflammatory neuropathies with different clinical courses but similar underlying mechanisms. Long non-coding RNAs (lncRNAs) might affect pathogenesis of these conditions. In the current project, we have selected HULC, PVT1, MEG3, SPRY4-IT1, LINC-ROR and DSCAM-AS1 lncRNAs to appraise their transcript levels in the circulation of CIDP and GBS cases versus controls. Expression of HULC was higher in CIDP patients compared with healthy persons (Ratio of mean expression (RME) = 7.62, SE = 0.72, P < 0.001). While expression of this lncRNA was not different between female CIDP cases and female controls, its expression was higher in male CIDP cases compared with male controls (RME = 13.50, SE = 0.98, P < 0.001). Similarly, expression of HULC was higher in total GBS cases compared with healthy persons (RME = 4.57, SE = 0.65, P < 0.001) and in male cases compared with male controls (RME = 5.48, SE = 0.82, P < 0.001). Similar pattern of expression was detected between total cases and total controls. PVT1 was up-regulated in CIDP cases compared with controls (RME = 3.04, SE = 0.51, P < 0.001) and in both male and female CIDP cases compared with sex-matched controls. Similarly, PVT1 was up-regulated in GBS cases compared with controls (RME = 2.99, SE = 0.55, P vale < 0.001) and in total patients compared with total controls (RME = 3.02, SE = 0.43, P < 0.001). Expression levels of DSCAM-AS1 and SPRY4-IT1 were higher in CIDP and GBS cases compared with healthy subjects and in both sexes compared with gender-matched healthy persons. Although LINC-ROR was up-regulated in total CIDP and total GBS cases compared with controls, in sex-based comparisons, it was only up-regulated in male CIDP cases compared with male controls (RME = 3.06, P = 0.03). Finally, expression of MEG3 was up-regulated in all subgroups of patients versus controls except for male GBS controls. SPRY4-IT could differentiate CIDP cases from controls with AUC = 0.84, sensitivity = 0.63 and specificity = 0.97. AUC values of DSCAM-AS1, MEG3, HULC, PVT1 and LINC-ROR were 0.80, 0.75, 0.74, 0.73 and 0.72, respectively. In differentiation between GBS cases and controls, SPRY4-IT and DSCAM-AS1 has the AUC value of 0.8. None of lncRNAs could appropriately differentiate between CIDP and GBS cases. Combination of all lncRNAs could not significantly enhance the diagnostic power. Taken together, these lncRNAs might be involved in the development of CIDP or GBS.
format	article
author	Mahdi Gholipour Mohammad Taheri Jafar Mehvari Habibabadi Naghme Nazer Arezou Sayad Soudeh Ghafouri-Fard
author_facet	Mahdi Gholipour Mohammad Taheri Jafar Mehvari Habibabadi Naghme Nazer Arezou Sayad Soudeh Ghafouri-Fard
author_sort	Mahdi Gholipour
title	Dysregulation of lncRNAs in autoimmune neuropathies
title_short	Dysregulation of lncRNAs in autoimmune neuropathies
title_full	Dysregulation of lncRNAs in autoimmune neuropathies
title_fullStr	Dysregulation of lncRNAs in autoimmune neuropathies
title_full_unstemmed	Dysregulation of lncRNAs in autoimmune neuropathies
title_sort	dysregulation of lncrnas in autoimmune neuropathies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/49cf2d6a751149ecaab4353a9259880a
work_keys_str_mv	AT mahdigholipour dysregulationoflncrnasinautoimmuneneuropathies AT mohammadtaheri dysregulationoflncrnasinautoimmuneneuropathies AT jafarmehvarihabibabadi dysregulationoflncrnasinautoimmuneneuropathies AT naghmenazer dysregulationoflncrnasinautoimmuneneuropathies AT arezousayad dysregulationoflncrnasinautoimmuneneuropathies AT soudehghafourifard dysregulationoflncrnasinautoimmuneneuropathies
_version_	1718377444538318848

Dysregulation of lncRNAs in autoimmune neuropathies

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